Figure 2 Measurement and simulation. (A) Schematic representation of the reactions included in the kinetic model. For clarity, the oligomers a, b and Inh have been omitted when not bound to a template. (B) Fluorescence signal (top panel, dashed line) and discrete concentration measurements of total individual oligomers a, b and Inh taken between t1⁄4235 and 520min (Supplementary information S3). The plots show the mean of three replicates±s.d. The solid curves are the best simultaneous agreement (‘optimized model’) for fluorescence signal and oligomer concentrations, respectively (Supplementary information S5). Molecular Systems Biology 7; Article number 476; doi:10.1038/msb.2011.12 Citation: Molecular Systems Biology 7:476 & 2011 EMBO and Macmillan Publishers Limited All rights reserved 1744-4292/11 www.molecularsystemsbiology.com