Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene

The gene for cyclodextrin glucanotransferase from Bacillus macerans was cloned in an Escherichia coli bacteriophage, lambda D69, and was recloned in a Bacillus subtilis plasmid, pUB110. Starting from an ATG initiation codon, a unique reading frame was shown to extend for 2,142 base pairs (714 amino acids). The nucleotide sequence revealed that the enzyme is composed of two identical subunits.

[1]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[2]  H. Tabak,et al.  A method for the recovery of DNA from agarose gels. , 1978, Nucleic acids research.

[3]  L. L. Campbell,et al.  Purification and properties of the amylase of Bacillus macerans. , 1968, Biochemistry.

[4]  W. E. TREVELYAN,et al.  Detection of Sugars on Paper Chromatograms , 1950, Nature.

[5]  D. Ward,et al.  A bacteriophage lambda vector for cloning with BamHI and Sau3A. , 1982, Gene.

[6]  J. Szejtli.  Cyclodextrins in Food, Cosmetics and Toiletries , 1982 .

[7]  J. Chang.  Amino-terminal analysis of polypeptide using dimethylaminoazobenzene isothiocyanate. , 1980, Analytical biochemistry.

[8]  B. Alberts,et al.  Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. , 1970, Virology.

[9]  H. Birnboim,et al.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA. , 1979, Nucleic acids research.

[10]  K. Yamane,et al.  Alpha-amylase genes (amyR2 and amyE+) from an alpha-amylase-hyperproducing Bacillus subtilis strain: molecular cloning and nucleotide sequences , 1983, Journal of bacteriology.

[11]  S. Suzuki,et al.  Purification and some properties of Bacillus macerans cycloamylose (cyclodextrin) glucanotransferase. , 1978, Carbohydrate research.

[12]  F. Kawamura,et al.  CONSTRUCTION AND PHYSICAL MAP OF A BACILLUS SUBTILIS SPECIALIZED TRANSDUCING PHAGE ρ11 CONTAINING B. SUBTILIS LYS+ GENE , 1982 .