Erythropoietin excretion in normal man.

Levels of erythropoietin have been consistently demonstrated in urine collections from healthy adult males using a modification of the polycythemic mouse assay. This method is stable and reproducible, and it provides assay animals with low background erythropoiesis. Six hour collections of urine can be concentrated without apparent loss of activity and the entire daily excretion can be used in the assay. Quantitation has been achieved by comparing the response to a concentrate of normal urine directly to a linear dose/response curve of erythropoietin standard B. Based on 6 hour urine collections, erythropoietin excretion in the subjects tested averaged 4.0 units of erythropoietin standard B (range 1.2-9.5). In addition to wide unexplained variations in results seen from day to day, the data indicate that a diurnal pattern of excretion of the hormone may exist.

[1]  D. V. Van Dyke,et al.  Erythropoietin in the urine of normal and erythropoietically abnormal human beings. , 1966, Blood.

[2]  P. Finne.  Erythropoietin in Concentrates of Urine from Healthy Persons , 1965, British medical journal.

[3]  E. Mirand,et al.  Erythropoietic Activity of Untreated and Deproteinized Normal Human Plasma.∗ , 1965, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[4]  J. Schooley,et al.  SOME PROPERTIES OF SERUM OBTAINED FROM RABBITS IMMUNIZED WITH HUMAN URINARY ERYTHROPOIETIN. , 1965, Blood.

[5]  W. Noyes,et al.  INCORPORATION OF RADIOIRON INTO MARROW HEME. , 1964, The Journal of laboratory and clinical medicine.

[6]  A. S. Gordon,et al.  USE OF THE HYPOXIA-INDUCED POLYCYTHEMIC MOUSE IN THE ASSAY AND STANDARDIZATION OF ERYTHROPOIETIN. , 1963, The Journal of laboratory and clinical medicine.

[7]  J. Labardini,et al.  Hemolysis and erythropoiesis. I. Influence of intraperitoneal administration of whole hemolysates on the recovery of bled dogs, as measured by changes in the total erythrocytic volume. , 1963, Blood.

[8]  J. Cooper,et al.  Erythropoietic Effect of Red Blood Cell Components and Heme-Related Compounds , 1963, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[9]  R. Degowin,et al.  The mouse with hypoxia-induced erythremia, an erythropoietin bioassay animal. , 1962, The Journal of laboratory and clinical medicine.

[10]  D. Bangham,et al.  Bio-Assay of Erythropoietin in Mice Made PolycythÆmic by Exposure to Air at a Reduced Pressure , 1961, Nature.

[11]  C. Gurney,et al.  Studies on erythropoiesis. XVII. Some quantitative aspects of the erythropoietic response to erythropoietin. , 1961, Blood.

[12]  A. Steinberg,et al.  The hematocrit of capillary blood. , 1955, The New England journal of medicine.

[13]  D. V. Slyke,et al.  Quantitative clinical chemistry , 1931 .