Effects of poly(lactic-co-glycolic acid) (PLGA) degradability on the apatite-forming capacity of electrospun PLGA/SiO(2)-CaO nonwoven composite fabrics.

We investigated the effects of poly(lactic-co-glycolic acid) (PLGA) degradability on the apatite-forming ability of electrospun PLGA/SiO(2)-CaO gel composite fabric. Two PLGA copolymer compositions with low and high degradability were used in experiments. A nonwoven polymer/ceramic composite fabric composed of randomly mixed microsized biodegradable PLGA fibers and nanosized bioactive SiO(2)-CaO gel fibers was prepared using a simultaneous electrospinning method. A 17 wt.% PLGA solution was prepared using 1,1,3,3-hexafluoro-2-propanol as a solvent, while the SiO(2)-CaO gel solution was prepared via a condensation reaction following hydrolysis of tetraethyl orthosilicate under acidic conditions. PLGA and SiO(2)-CaO gel solutions were spun simultaneously with two separate nozzles under electric fields of 1 and 2 kV/cm using two syringe pumps with flow rates of 7.5 and 5 mL/h, respectively. As controls, low and high degradable PLGA and SiO(2)-CaO gel nonwoven fabrics were also made by the same methods. The five nonwoven fabrics that were produced were exposed to simulated body fluid (SBF) for 1 week. SBF exposure resulted in the deposition of a layer of apatite crystals on the surfaces of both the SiO(2)-CaO gel and the low degradable PLGA/SiO(2)-CaO gel composite fabrics, but not on the low and high degradable PLGA or the high degradable PLGA/SiO(2)-CaO gel composite fabrics. The results are explained in terms of the acidity of the PLGA degradation products, which could have a direct influence on apatite dissolution.

[1]  Young-Mi Kang,et al.  Bioactivity, pre-osteoblastic cell responses, and osteoconductivity evaluations of the electrospun non-woven SiO2-CaO gel fabrics. , 2009, Journal of biomedical materials research. Part B, Applied biomaterials.

[2]  Casey K Chan,et al.  The fabrication of nano-hydroxyapatite on PLGA and PLGA/collagen nanofibrous composite scaffolds and their effects in osteoblastic behavior for bone tissue engineering. , 2009, Bone.

[3]  R L Reis,et al.  Nucleation and growth of biomimetic apatite layers on 3D plotted biodegradable polymeric scaffolds: effect of static and dynamic coating conditions. , 2009, Acta biomaterialia.

[4]  W. Stark,et al.  In vivo and in vitro evaluation of flexible, cottonwool-like nanocomposites as bone substitute material for complex defects. , 2009, Acta Biomaterialia.

[5]  V. Denaro,et al.  Poly-l-Lactic Acid/Hydroxyapatite Electrospun Nanocomposites Induce Chondrogenic Differentiation of Human MSC , 2009, Annals of Biomedical Engineering.

[6]  S. Ramakrishna,et al.  Nanostructured biocomposite substrates by electrospinning and electrospraying for the mineralization of osteoblasts. , 2009, Biomaterials.

[7]  D. Guan,et al.  Attachment, proliferation and differentiation of BMSCs on gas-jet/electrospun nHAP/PHB fibrous scaffolds , 2008 .

[8]  Seeram Ramakrishna,et al.  Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering. , 2008, Biomaterials.

[9]  D. Kalyon,et al.  Functionally graded electrospun polycaprolactone and beta-tricalcium phosphate nanocomposites for tissue engineering applications. , 2008, Biomaterials.

[10]  A. Stanishevsky,et al.  Hydroxyapatite nanoparticle loaded collagen fiber composites: microarchitecture and nanoindentation study. , 2008, Journal of biomedical materials research. Part A.

[11]  S. Ramakrishna,et al.  Nanobioengineered electrospun composite nanofibers and osteoblasts for bone regeneration. , 2008, Artificial organs.

[12]  S. Ramakrishna,et al.  Mineralization of osteoblasts with electrospun collagen/hydroxyapatite nanofibers , 2008, Journal of materials science. Materials in medicine.

[13]  W. Stark,et al.  Cotton wool-like nanocomposite biomaterials prepared by electrospinning: in vitro bioactivity and osteogenic differentiation of human mesenchymal stem cells. , 2008, Journal of biomedical materials research. Part B, Applied biomaterials.

[14]  S. Rhee,et al.  Effect of acidic degradation products of poly(lactic-co-glycolic)acid on the apatite-forming ability of poly(lactic-co-glycolic)acid-siloxane nanohybrid material. , 2007, Journal of biomedical materials research. Part A.

[15]  Shaobing Zhou,et al.  In situ growth of hydroxyapatite within electrospun poly(DL-lactide) fibers. , 2007, Journal of biomedical materials research. Part A.

[16]  G. Sui,et al.  Poly-L-lactic acid/hydroxyapatite hybrid membrane for bone tissue regeneration. , 2007, Journal of biomedical materials research. Part A.

[17]  P. Supaphol,et al.  Osteoblastic phenotype expression of MC3T3-E1 cultured on electrospun polycaprolactone fiber mats filled with hydroxyapatite nanoparticles. , 2007, Biomacromolecules.

[18]  A. Stanishevsky,et al.  An electrospun triphasic nanofibrous scaffold for bone tissue engineering , 2007, Biomedical materials.

[19]  Seeram Ramakrishna,et al.  Biocomposite nanofibres and osteoblasts for bone tissue engineering , 2007 .

[20]  Derrick Dean,et al.  Nanostructured Biomaterials for Regenerative Medicine , 2006 .

[21]  Byung-Soo Kim,et al.  Poly(lactide-co-glycolide)/hydroxyapatite composite scaffolds for bone tissue engineering. , 2006, Biomaterials.

[22]  J. Goh,et al.  Characterization of a novel polymeric scaffold for potential application in tendon/ligament tissue engineering. , 2006, Tissue engineering.

[23]  Wei He,et al.  Grafting of gelatin on electrospun poly(caprolactone) nanofibers to improve endothelial cell spreading and proliferation and to control cell Orientation. , 2005, Tissue engineering.

[24]  Sheila MacNeil,et al.  Self-organization of skin cells in three-dimensional electrospun polystyrene scaffolds. , 2005, Tissue engineering.

[25]  Jun Yao,et al.  The effect of bioactive glass content on synthesis and bioactivity of composite poly (lactic-co-glycolic acid)/bioactive glass substrate for tissue engineering. , 2005, Biomaterials.

[26]  Seeram Ramakrishna,et al.  Surface engineering of electrospun polyethylene terephthalate (PET) nanofibers towards development of a new material for blood vessel engineering. , 2005, Biomaterials.

[27]  R. Tuan,et al.  A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells. , 2005, Biomaterials.

[28]  M. Kotaki,et al.  Aligned biodegradable nanofibrous structure: a potential scaffold for blood vessel engineering. , 2004, Biomaterials.

[29]  J. Vacanti,et al.  A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. , 2003, Biomaterials.

[30]  B. Hsiao,et al.  Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA-PEG block copolymers. , 2003, Journal of controlled release : official journal of the Controlled Release Society.

[31]  N. Miyata,et al.  Apatite-forming ability and mechanical properties of CaO-free poly(tetramethylene oxide) (PTMO)-TiO2 hybrids treated with hot water. , 2003, Biomaterials.

[32]  Je-Yong Choi,et al.  Preparation of a bioactive and degradable poly(ε-caprolactone)/silica hybrid through a sol–gel method , 2002 .

[33]  M. Tanihara,et al.  Development of bioactive organic–inorganic hybrid for bone substitutes , 2002 .

[34]  John Layman,et al.  Release of tetracycline hydrochloride from electrospun poly(ethylene-co-vinylacetate), poly(lactic acid), and a blend. , 2002, Journal of controlled release : official journal of the Controlled Release Society.

[35]  N. Miyata,et al.  Effect of heat treatment on bioactivity and mechanical properties of PDMS-modified CaO-SiO2-TiO2 hybrids via sol-gel process , 2001, Journal of materials science. Materials in medicine.

[36]  A. Seddon,et al.  Near- and mid-infrared spectroscopy of sol–gel derived ormosils: vinyl and phenyl silicates , 1997 .

[37]  Chikara Ohtsuki,et al.  Dependence of apatite formation on silica gel on its structure : effect of heat treatment , 1995 .

[38]  A. U. Daniels,et al.  Six bioabsorbable polymers: in vitro acute toxicity of accumulated degradation products. , 1994, Journal of applied biomaterials : an official journal of the Society for Biomaterials.

[39]  Chikara Ohtsuki,et al.  Chemical reaction of bioactive glass and glass-ceramics with a simulated body fluid , 1992 .

[40]  A. Pennings,et al.  Porous polymer implant for repair of meniscal lesions: a preliminary study in dogs. , 1991, Biomaterials.

[41]  T Kitsugi,et al.  Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W. , 1990, Journal of biomedical materials research.

[42]  J. Voegel,et al.  Biological Apatite Crystal Dissolution , 1979, Journal of dental research.

[43]  Young-Mi Kang,et al.  Evaluations of osteogenic and osteoconductive properties of a non-woven silica gel fabric made by the electrospinning method. , 2009, Acta biomaterialia.

[44]  Chikara Ohtsuki,et al.  Mechanism of apatite formation on CaOSiO2P2O5 glasses in a simulated body fluid , 1992 .

[45]  O. Böstman.  Absorbable implants for the fixation of fractures. , 1991, The Journal of bone and joint surgery. American volume.