The Effect of Inhibin Immunization in Seminiferous Epithelium of Yangzhou Goose Ganders: A Histological Study

Simple Summary Achieving optimum fertility is the ultimate goal of keeping gander flock in commercial geese farming. Being seasonal breeders, geese exhibit poor reproductive efficiency. Inhibin (INH) immunization has improved reproductive parameters in some mammalian species but remained obscure in birds, especially geese. The present study aimed to check the effect of INH immunization on testicular histoarchitecture of Yangzhou ganders. Results illustrated that INH immunization disrupted seminiferous epithelium, germ cells development, lowered efficiency of spermatogenesis, and caused apoptosis in seminiferous tubules of Yangzhou ganders. Abstract The current study investigated the effect of inhibin immunization on germ cell numbers (spermatogonia, spermatocytes, round, and elongated spermatids), seminiferous tubules (ST) diameter, Johnsen’s score, epithelial height (μm), luminal tubular diameter (μm), and number of ST per field (ST/field) of Yangzhou goose ganders. Histological evaluation showed apoptosis and regression of testes after inhibin (INH) immunization, with a concomitantly marked reduction in the round and elongated spermatids in the experiment (INH) group compared to the control group. The diameter of seminiferous tubules (ST) and epithelial height (EH) were positively correlated at 181, 200, and 227 days of age. In comparison, luminal tubular diameter (LD) was negatively correlated on day 227 to ST diameter and epithelial height. On day 227, many seminiferous tubules per field (ST/field) were negatively correlated to ST diameter, EH, and LD. INH immunization elevated ST diameter, EH, and LD, while Johnsen’s score and number of ST/field had reciprocal expression. In conclusion, the concomitant effect of INH immunization and seasonality in breeding regressed germ cells and damaged spermatogenesis in seminiferous epithelium Yangzhou ganders.

[1]  A. Sattar,et al.  Long term effects of immunization against inhibin on fresh and post-thawed semen quality and sperm kinematics during low and peak breeding seasons in Beetal bucks , 2021 .

[2]  I. Mohsin,et al.  Effects of immunization against inhibin α-subunit on ovarian structures, pregnancy rate, embryonic and fetal losses, and prolificacy rate in goats where estrus was induced during the non-breeding season. , 2020, Animal reproduction science.

[3]  F. Shi,et al.  Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders , 2020, Animals : an open access journal from MDPI.

[4]  Wan-Xi Yang,et al.  Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis , 2019, Cell Death & Disease.

[5]  Zuping He,et al.  The roles and mechanisms of Leydig cells and myoid cells in regulating spermatogenesis , 2019, Cellular and Molecular Life Sciences.

[6]  Q. Wei,et al.  The role of active immunization against inhibin α-subunit on testicular development, testosterone concentration and relevant genes expressions in testis, hypothalamus and pituitary glands in Yangzhou goose ganders. , 2019, Theriogenology.

[7]  C. Chaturvedi,et al.  Apoptotic mechanism behind the testicular atrophy in photorefractory and scotosensitive quail: Involvement of GnIH induced p-53 dependent Bax-Caspase-3 mediated pathway. , 2017, Journal of photochemistry and photobiology. B, Biology.

[8]  JianNing Yu,et al.  Reproductiveaxis gene regulation during photostimulation and photorefractoriness in Yangzhou goose ganders , 2017, Frontiers in Zoology.

[9]  D. Mao,et al.  Effect of inhibin gene immunization on antibody production and reproductive performance in Partridge Shank hens. , 2016, Theriogenology.

[10]  Xiangguo Wang,et al.  Establishment and evaluation of a stable steroidogenic goat Leydig cell line. , 2016, Animal science journal = Nihon chikusan Gakkaiho.

[11]  S. Sugita,et al.  Apoptosis-mediated seasonal testicular regression in the Japanese Jungle Crow (Corvus macrorhynchos). , 2012, Theriogenology.

[12]  B. Jamieson.  Reproductive Biology and Phylogeny of Birds, Part A : Phylogeny, Morphology, Hormones and Fertilization , 2011 .

[13]  G. Hua,et al.  Action Mechanism of Inhibin α-Subunit on the Development of Sertoli Cells and First Wave of Spermatogenesis in Mice , 2011, PloS one.

[14]  Y. Chaiseha,et al.  The effect of active immunization against vasoactive intestinal peptide (VIP) and inhibin on reproductive performance of aging White Leghorn roosters. , 2011, Poultry science.

[15]  Yuan Tian,et al.  Controlling reproductive seasonality in the geese: a review , 2008 .

[16]  J. E. Wheaton,et al.  Effects of immunization against two inhibin antigens on daily sperm production and hormone concentrations in ram lambs. , 2007, Journal of animal science.

[17]  K. Young,et al.  Increases in apoptosis and declines in Bcl-XL protein characterise testicular regression in American crows (Corvus brachyrhynchos). , 2007, Reproduction, fertility, and development.

[18]  E. Nieschlag,et al.  Inhibin B in male reproduction: pathophysiology and clinical relevance. , 2001, European journal of endocrinology.

[19]  Gregory F Ball,et al.  Photoperiod-Induced Testicular Apoptosis in European Starlings (Sturnus vulgaris)1 , 2001, Biology of reproduction.

[20]  D. G. Satterlee,et al.  Active immunization of Japanese quail hens with a recombinant chicken inhibin fusion protein enhances production performance. , 1998, Poultry science.

[21]  P. G. Knight.  Roles of Inhibins, Activins, and Follistatin in the Female Reproductive System , 1996, Frontiers in Neuroendocrinology.

[22]  A. Grootenhuis,et al.  Inhibin reduces spermatogonial numbers in testes of adult mice and Chinese hamsters. , 1989, Endocrinology.

[23]  S. Ying.  Inhibins, activins, and follistatins: gonadal proteins modulating the secretion of follicle-stimulating hormone. , 1988, Endocrine reviews.

[24]  A. Møller.  Testes size, ejaculate quality and sperm competition in birds , 1988 .

[25]  L. Russell,et al.  Hormonal control of pubertal spermatogenesis. , 1987, Endocrinology.

[26]  P. Péczely,et al.  Effect of low light intensity on testicular function in photorefractory domestic ganders. , 1985, General and comparative endocrinology.

[27]  J. Dittami,et al.  Seasonal changes in follicle-stimulating hormone in a breeding population of barheaded geese, Anser indicus. , 1985, General and comparative endocrinology.

[28]  施振旦.  Boosting the Economic Returns of Goose Breeding and Developing the Industry by Controlled Photoperiod for Out-of-SeasonReproduction , 2015 .

[29]  Y. Chaiseha,et al.  The effect of active immunization against vasoactive intestinal peptide and inhibin on reproductive performance of young White Leghorn roosters. , 2011, Poultry science.

[30]  B. Schanbacher.  Pituitary and testicular responses of beef bulls to active immunization against inhibin alpha. , 1991, Journal of animal science.

[31]  D. Kerrigan,et al.  A MODIFIED JOHNSEN’S COUNT FOR EVALUATION OF SPERMATOGENESIS IN THE RAT , 1985 .

[32]  M. D. de Reviers,et al.  [Autoradiography and tritiated thymidine labeling during spermatogenesis of the Barbary Duck (Cairina moschata L.)]. , 1977, Comptes rendus des seances de la Societe de biologie et de ses filiales.