Structural and kinetic effects of mobile phone microwaves on acetylcholinesterase activity.

The present study provides evidence that "in vitro" simple exposure of an aqueous solution of electric eel acetylcholinesterase (EeAChE; EC 3.1.1.7.) to cellular phone emission alters its enzymatic activity. This paper demonstrates, by combining different experimental techniques, that radio frequency (RF) radiations irreversibly affect the structural and biochemical characteristics of an important CNS enzyme. These results were obtained by using a commercial cellular phone to reproduce the reality of the human exposition. This experimental procedure provided surprising effects collected practically without experimental errors because they were obtained comparing native and irradiated sample of the same enzyme solution. Although these results cannot be used to conclude whether exposure to RF during the use of cellular phone can lead to any hazardous health effect, they may be a significant first step towards further verification of these effects on other "ex vivo" or "in vivo" biological systems.

[1]  A. Guy,et al.  Low-level microwave irradiation and central cholinergic activity: a dose-response study. , 1989, Bioelectromagnetics.

[2]  P. Dimbylow,et al.  SAR calculations in an anatomically realistic model of the head for mobile communication transceivers at 900 MHz and 1.8 GHz. , 1994, Physics in medicine and biology.

[3]  R. Blinc,et al.  Proton NMR relaxation of adsorbed water in gelatin and collagen , 1995 .

[4]  Masao Kakudo,et al.  Small Angle Scattering of X-Rays , 1968 .

[5]  R L Blakeley,et al.  Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination. , 1979, Analytical biochemistry.

[6]  A. Guy,et al.  Low‐Level Microwave Irradiations Affect Central Cholinergic Activity in the Rat , 1987, Journal of neurochemistry.

[7]  R. D. Saunders.  Biological effects of exposure to non-ionizing electromagnetic fields and radiation, III. Radiofrequency and microwave radiation , 1991 .

[8]  J. Poorter,et al.  Calculation of the electromagnetic fields induced in the head of an operator of a cordless telephone , 1995 .

[9]  W. Joines,et al.  The effect of microwave radiation (1.0 GHz) on the blood‐brain barrier in dogs , 1982 .

[10]  J. Bonner,et al.  Differentiation , 1968, Nature.

[11]  Dariusz Leszczynski,et al.  Non-thermal activation of the hsp27/p38MAPK stress pathway by mobile phone radiation in human endothelial cells: molecular mechanism for cancer- and blood-brain barrier-related effects. , 2002, Differentiation; research in biological diversity.

[12]  John B. Shoven,et al.  I , Edinburgh Medical and Surgical Journal.

[13]  R. Blinc,et al.  Proton NMR study of the state of water in fibrin gels, plasma, and blood clots , 1990, Magnetic resonance in medicine.

[14]  M. L. Jones,et al.  PDBsum: a Web-based database of summaries and analyses of all PDB structures. , 1997, Trends in biochemical sciences.

[15]  J L Sussman,et al.  Specific chemical and structural damage to proteins produced by synchrotron radiation. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[16]  J. Warwicker,et al.  Calculation of the electric potential in the active site cleft due to alpha-helix dipoles. , 1982, Journal of molecular biology.

[17]  J. Griffiths.  Nuclear Magnetic Resonance and its Applications to Living Systems , 1982 .

[18]  F. Young.  Biochemistry , 1955, The Indian Medical Gazette.

[19]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[20]  K. Sharp,et al.  Calculating the electrostatic potential of molecules in solution: Method and error assessment , 1988 .

[21]  A. Guy,et al.  Acute low-level microwave exposure and central cholinergic activity: studies on irradiation parameters. , 1988, Bioelectromagnetics.

[22]  宁北芳,et al.  疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A , 2005 .

[23]  I. Wilson,et al.  A PARTIAL CHARACTERIZATION OF ACETYLCHOLINESTERASE. , 1964, Biochemistry.

[24]  S. Spragg.  Biophysical chemistry , 1979, Nature.

[25]  S. Lifson,et al.  External and internal electrostatic potentials of cholinesterase models. , 1997, Journal of molecular graphics & modelling.

[26]  F. Gianturco,et al.  Rotational energy transfer in ion-molecule scattering. I : The proton-water example , 1991 .