Further Notes on the Overwintering of the Eggs of Anopheles walkeri Theobald with a Description of the Eggs.

The overwintering eggs were obtained September 5-23 from four wild specimens collected at Ithaca, New York. The mosquitoes were confined individually, for oviposition, in small jars in the laboratory. The eggs of each batch were removed from the jar within 24 hours and placed out-of-doors on the roof of a low north wing, and thus were protected from direct sunlight while exposed to out-of-door temperature. They were left there until spring. Both small enameled cups and Syracuse watch glasses were used as containers, each one being covered with a glass cover to retard evaporation. They were all placed under a small glass aquarium as protection against wind, snow and rain. The eggs were placed on water in the containers and confined within waxed paper rings which floated on the surface. The temperature data given are air temperatures which have been secured from the records of the U. S. Weather Bureau Station at Ithaca, which is about 100 yards distant.