Antibacterial nitric oxide-releasing polyester for the coating of blood-contacting artificial materials.

The emergence of multidrug-resistant bacteria associated with blood-contacting artificial materials is a growing health problem, which demands new approaches in the field of biomaterials research. In this study, a poly(sulfhydrylated polyester) (PSPE) was synthesized by the polyesterification reaction of mercaptosuccinic acid with 3-mercapto-1,2-propanediol and blended with poly(methyl methacrylate) (PMMA) from solution, leading to solid PSPE/PMMA films, with three different PSPE : PMMMA mass ratios. These films were subsequently S-nitrosated through the immersion in acidified nitrite solution, yielding poly(nitrosated)polyester/PMMA (PNPE/PMMA) films. A polyurethane intravascular catheter coated with PNPE/PMMA was shown to release nitric oxide (NO) in phosphate buffered saline solution (pH 7.4) at 37 degrees C at rates of 4.6 nmol/cm(2)/h in the first 6 h and 0.8 nmol/cm(2)/h in the next 12 h. When used to coat the bottom of culture plates, NO released from these films exerted a potent dose- and time-dependent antimicrobial activity against Staphylococcus aureus and a multidrug-resistant Pseudomonas aeruginosa strains. This antibacterial effect of PSPE/PMMA films opens a new perspective for the coating of blood-contacting artificial materials, for avoiding their colonization with highly resistant bacteria.

[1]  E. Lazar,et al.  Antifungal effect of gaseous nitric oxide on mycelium growth, sporulation and spore germination of the postharvest horticulture pathogens, Aspergillus niger, Monilinia fructicola and Penicillium italicum , 2008, Letters in applied microbiology.

[2]  A. Seabra,et al.  Antithrombogenic polynitrosated polyester/poly(methyl methacrylate) blend for the coating of blood-contacting surfaces. , 2008, Artificial organs.

[3]  C. Bifulco,et al.  Role for Nitric Oxide in Hookworm-Associated Immune Suppression , 2008, Infection and Immunity.

[4]  M. Schoenfisch,et al.  Bactericidal efficacy of nitric oxide-releasing silica nanoparticles. , 2008, ACS nano.

[5]  Cristina L. M. Silva,et al.  Ethylene oxide sterilization of medical devices: a review. , 2007, American journal of infection control.

[6]  J. Essigmann,et al.  Chemical-biological fingerprinting: probing the properties of DNA lesions formed by peroxynitrite. , 2007, Chemical research in toxicology.

[7]  Siddaramaiah,et al.  Thermal and mechanical properties of poly(methyl methacrylate) and ethylene vinyl acetate copolymer blends , 2007 .

[8]  Benjamin A Lipsky,et al.  Skin, Soft Tissue, Bone, and Joint Infections in Hospitalized Patients: Epidemiology and Microbiological, Clinical, and Economic Outcomes , 2007, Infection Control & Hospital Epidemiology.

[9]  R. Duval,et al.  In vitro activity of para-guanidinoethylcalix[4]arene against susceptible and antibiotic-resistant Gram-negative and Gram-positive bacteria. , 2007, The Journal of antimicrobial chemotherapy.

[10]  B. Markova,et al.  Problematic clinical isolates of Pseudomonas aeruginosa from the university hospitals in Sofia, Bulgaria: current status of antimicrobial resistance and prevailing resistance mechanisms. , 2007, Journal of medical microbiology.

[11]  W. Jarvis.  The Lowbury Lecture. The United States approach to strategies in the battle against healthcare-associated infections, 2006: transitioning from benchmarking to zero tolerance and clinician accountability. , 2007, The Journal of hospital infection.

[12]  M. Schoenfisch,et al.  Antibacterial nitric oxide-releasing xerogels: cell viability and parallel plate flow cell adhesion studies. , 2007, Biomaterials.

[13]  D. C. Miguel,et al.  Leishmanicidal activity of primary S-nitrosothiols against Leishmania major and Leishmania amazonensis: implications for the treatment of cutaneous leishmaniasis. , 2006, Nitric oxide : biology and chemistry.

[14]  B. Allegranzi,et al.  'Clean Care is Safer Care': the Global Patient Safety Challenge 2005-2006. , 2006, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases.

[15]  D. Hassett,et al.  Involvement of Nitric Oxide in Biofilm Dispersal of Pseudomonas aeruginosa , 2006, Journal of bacteriology.

[16]  J. Boyce,et al.  Emergence and resurgence of meticillin-resistant Staphylococcus aureus as a public-health threat , 2006, The Lancet.

[17]  M. Schoenfisch,et al.  Poly(vinylpyrrolidone)-doped nitric oxide-releasing xerogels as glucose biosensor membranes. , 2006, Biosensors & bioelectronics.

[18]  Q. Qi,et al.  Delivery of Nitric Oxide Released from β-Gal-NONOate Activation by β-Galactosidase and Its Activity against Escherichia coli , 2006 .

[19]  M. Trujillo,et al.  Mechanistic studies of peroxynitrite-mediated tyrosine nitration in membranes using the hydrophobic probe N-t-BOC-L-tyrosine tert-butyl ester. , 2006, Biochemistry.

[20]  A. Ghahary,et al.  Potential application of gaseous nitric oxide as a topical antimicrobial agent. , 2006, Nitric oxide : biology and chemistry.

[21]  M. Schoenfisch,et al.  Inhibition of implant-associated infections via nitric oxide release. , 2005, Biomaterials.

[22]  A. Seabra,et al.  Polynitrosated polyesters: preparation, characterization, and potential use for topical nitric oxide release. , 2005, Biomacromolecules.

[23]  M. Gladwin,et al.  Erythrocytes are the major intravascular storage sites of nitrite in human blood. , 2005, Blood.

[24]  P. Wiklund,et al.  Intravesical Nitric Oxide Delivery for Prevention of Catheter-Associated Urinary Tract Infections , 2005, Antimicrobial Agents and Chemotherapy.

[25]  M. Eberlin,et al.  Solid films of blended poly(vinyl alcohol)/poly(vinyl pyrrolidone) for topical S-nitrosoglutathione and nitric oxide release. , 2005, Journal of pharmaceutical sciences.

[26]  J. Imlay,et al.  Repair of Oxidized Iron-Sulfur Clusters in Escherichia coli* , 2004, Journal of Biological Chemistry.

[27]  M. Schoenfisch,et al.  Antibacterial properties of nitric oxide-releasing sol-gel microarrays. , 2004, Biomacromolecules.

[28]  A. Seabra,et al.  Poly(vinyl alcohol) and poly(vinyl pyrrolidone) blended films for local nitric oxide release. , 2004, Biomaterials.

[29]  Rafael Radi,et al.  Nitric oxide, oxidants, and protein tyrosine nitration , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[30]  N. Morgon,et al.  Thermal Stability of Primary S-Nitrosothiols: Roles of Autocatalysis and Structural Effects on the Rate of Nitric Oxide Release , 2002 .

[31]  H. Maeda,et al.  Role of Nitric Oxide in Host Defense in Murine Salmonellosis as a Function of Its Antibacterial and Antiapoptotic Activities , 2002, Infection and Immunity.

[32]  G. Stefano,et al.  Tonal nitric oxide and health: anti-bacterial and -viral actions and implications for HIV. , 2002, Medical science monitor : international medical journal of experimental and clinical research.

[33]  R. Wenzel,et al.  The impact of hospital-acquired bloodstream infections. , 2001, Emerging infectious diseases.

[34]  P. Nair.  Currently Practised Sterilization Methods-Some Inadvertent Consequences , 1995, Journal of biomaterials applications.

[35]  J. Davies,et al.  Optimizing the sterilization of PLGA scaffolds for use in tissue engineering. , 2001, Biomaterials.

[36]  B. Zerner,et al.  [8] Reassessment of Ellman's reagent , 1983 .