Nano-Photothermal ablation effect of Hydrophilic and Hydrophobic Functionalized Gold Nanorods on Staphylococcus aureus and Propionibacterium acnes

The potential photothermal bactericidal activity of hydrophilic functionalized poly ethylene glycol (PEG)-gold nanorods (GNR) and hydrophobic functionalized polystyrene (PS)-GNR was evaluated towards strains of Staphylococcus aureus (S. aureus) and Propionibacterium acnes (P. acnes) by measuring the percentage reduction of bacterial viable count upon GNR excitation with a near infra-red (NIR) laser beam. Our results suggest that functionalized GNR had a minimal bactericidal activity against S. aureus and P. acnes (≤85%, i.e. ≤1 log10 cycle reduction of bacterial viable count). However, the local heat generated upon exciting the functionalized GNR with NIR laser beam has a significant photothermal ablation effect (≥99.99%, i.e. ≥4 log10 cycles reduction of bacterial viable count). Such photothermolysis effect could potentiate the antibacterial activity of GNR, which may call for minimum concentration and side effects of these nanotherapeutics.

[1]  Mostafa A. El-Sayed,et al.  The Most Effective Gold Nanorod Size for Plasmonic Photothermal Therapy: Theory and In Vitro Experiments , 2014, The journal of physical chemistry. B.

[2]  Dhiraj Kumar,et al.  Polyethylene glycol functionalized gold nanoparticles: the influence of capping density on stability in various media , 2011 .

[3]  Lei Liu,et al.  Photothermal lysis of pathogenic bacteria by platinum nanodots decorated gold nanorods under near infrared irradiation. , 2018, Journal of hazardous materials.

[4]  Mauris N DeSilva,et al.  Photothermal killing of Staphylococcus aureus using antibody-targeted gold nanoparticles , 2015, International journal of nanomedicine.

[5]  F. Martínez-Gutiérrez,et al.  Antibacterial and antibiofilm activities of the photothermal therapy using gold nanorods against seven different bacterial strains , 2015 .

[6]  P. Kantoff,et al.  Cancer nanomedicine: progress, challenges and opportunities , 2016, Nature Reviews Cancer.

[7]  Manickkam Jayakumar,et al.  In vitro antibacterial activity of some plant essential oils , 2006, BMC complementary and alternative medicine.

[8]  C. Murphy,et al.  Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity. , 2005, Small.

[9]  Stephan Link,et al.  Optical characterization of single plasmonic nanoparticles. , 2015, Chemical Society reviews.

[10]  Vladimir P Zharov,et al.  Photothermal nanotherapeutics and nanodiagnostics for selective killing of bacteria targeted with gold nanoparticles. , 2006, Biophysical journal.

[11]  Vladimir P. Zharov,et al.  Photothermal and accompanied phenomena of selective nanophotothermolysis with gold nanoparticles and laser pulses , 2008 .

[12]  Alaaldin M. Alkilany,et al.  The gold standard: gold nanoparticle libraries to understand the nano-bio interface. , 2013, Accounts of chemical research.

[13]  Alaaldin M. Alkilany,et al.  Preferential accumulation of gold nanorods into human skin hair follicles: Effect of nanoparticle surface chemistry. , 2017, Journal of colloid and interface science.

[14]  David A Jaffray,et al.  Gold nanoparticles for applications in cancer radiotherapy: Mechanisms and recent advancements☆ , 2017, Advanced drug delivery reviews.

[15]  E. Winer,et al.  Randomized Phase III Trial of Paclitaxel Once Per Week Compared With Nanoparticle Albumin-Bound Nab-Paclitaxel Once Per Week or Ixabepilone With Bevacizumab As First-Line Chemotherapy for Locally Recurrent or Metastatic Breast Cancer: CALGB 40502/NCCTG N063H (Alliance). , 2015, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[16]  Alaaldin M. Alkilany,et al.  Antibacterial activity of gold nanorods against Staphylococcus aureus and Propionibacterium acnes: misinterpretations and artifacts , 2017, International journal of nanomedicine.

[17]  M. J. Kim,et al.  Rapid photothermal lysis of the pathogenic bacteria, Escherichia coli using synthesis of gold nanorods. , 2009, Journal of nanoscience and nanotechnology.

[18]  Xiaohua Huang,et al.  Gold nanoparticles: Optical properties and implementations in cancer diagnosis and photothermal therapy , 2010 .

[19]  M. Komiyama,et al.  Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics , 2017 .

[20]  Prashant K. Jain,et al.  Plasmonic photothermal therapy (PPTT) using gold nanoparticles , 2008, Lasers in Medical Science.

[21]  Galina Maslyakova,et al.  Towards Effective Photothermal/Photodynamic Treatment Using Plasmonic Gold Nanoparticles , 2016, International journal of molecular sciences.

[22]  Teodora Mocan,et al.  Selective in vitro photothermal nano-therapy of MRSA infections mediated by IgG conjugated gold nanoparticles , 2016, Scientific Reports.

[23]  Miriam Rafailovich,et al.  Antimicrobial effects of TiO(2) and Ag(2)O nanoparticles against drug-resistant bacteria and leishmania parasites. , 2011, Future microbiology.

[24]  Vincent M. Rotello,et al.  Functional Gold Nanoparticles as Potent Antimicrobial Agents against Multi-Drug-Resistant Bacteria , 2014, ACS nano.

[25]  I. In,et al.  Light controllable surface coating for effective photothermal killing of bacteria. , 2015, ACS applied materials & interfaces.

[26]  S. Rannard,et al.  The application of nanotechnology in medicine: treatment and diagnostics. , 2014, Nanomedicine.

[27]  Y. Barenholz Doxil®--the first FDA-approved nano-drug: lessons learned. , 2012, Journal of controlled release : official journal of the Controlled Release Society.

[28]  Vincent M. Rotello,et al.  Tuning Payload Delivery in Tumour Cylindroids using Gold Nanoparticles , 2010, Nature nanotechnology.

[29]  Valery V Tuchin,et al.  Enhanced photoinactivation of Staphylococcus aureus with nanocomposites containing plasmonic particles and hematoporphyrin , 2013, Journal of biophotonics.

[30]  Michael J Sailor,et al.  Computationally guided photothermal tumor therapy using long-circulating gold nanorod antennas. , 2009, Cancer research.

[31]  S. Barry,et al.  Aurora kinase inhibitor nanoparticles target tumors with favorable therapeutic index in vivo , 2016, Science Translational Medicine.

[32]  Prashant K. Jain,et al.  Noble Metals on the Nanoscale: Optical and Photothermal Properties and Some Applications in Imaging, Sensing, Biology, and Medicine , 2009 .

[33]  B. Sirithunyalug,et al.  Antibacterial Activity and Inhibition of Adherence of Streptococcus mutans by Propolis Electrospun Fibers , 2014, AAPS PharmSciTech.

[34]  M. Salouti,et al.  Anti protein A antibody-gold nanorods conjugate: a targeting agent for selective killing of methicillin resistant Staphylococcus aureus using photothermal therapy method , 2015, Journal of Microbiology.

[35]  D. K. Yi,et al.  Antibacterial activity of ordered gold nanorod arrays. , 2014, ACS applied materials & interfaces.

[36]  K. Kimura,et al.  Widely Tunable Plasmon Resonances from Visible to Near-Infrared of Hollow Silver Nanoshells , 2017 .

[37]  Young Ha Kim,et al.  Photothermal Cancer Therapy and Imaging Based on Gold Nanorods , 2011, Annals of Biomedical Engineering.

[38]  Michael Hawkins,et al.  Phase III trial of nanoparticle albumin-bound paclitaxel compared with polyethylated castor oil-based paclitaxel in women with breast cancer. , 2005, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[39]  Younan Xia,et al.  Gold nanostructures: engineering their plasmonic properties for biomedical applications. , 2006, Chemical Society reviews.