Alterations in Kidney Structures Caused by Age Vary According to Sex and Dehydration Condition

Aging is a complex biological process, resulting in gradual and progressive decline in structure and function in many organ systems. Our objective is to determine if structural changes produced by aging vary with sex in a stressful situation such as dehydration. The expression of Slc12a3 mRNA in the renal cortex, α-smooth muscle actin (α-SMA), and fibronectin was evaluated in male and female rats, aged 3 and 18 months, submitted and not submitted to water deprivation (WD) for 48 h, respectively. When comparing ages, 18-month-old males showed a lower expression of Slc12a3 mRNA than 3-month-old males, and control and WD 18-month-old male and female rats exhibited a higher expression of α-SMA than the respective 3-month-old rats. Fibronectin was higher in both control and WD 18-month-old males than the respective 3-month-old males. In females, only the control 18-month-old rats showed higher fibronectin than the control 3-month-old rats. When we compared sex, control and WD 3-month-old female rats had a lower expression of Slc12a3 mRNA than the respective males. The WD 18-month-old male rats presented a higher expression of fibronectin and α-SMA than the WD 18-month-old female rats. When we compared hydric conditions, the WD 18-month-old males displayed a lower relative expression of Slc12a3 mRNA and higher α-SMA expression than the control 18-month-old males. Aging, sex, and dehydration lead to alterations in kidney structure.

[1]  J. Exbrayat,et al.  Kidney functional morphology variations between spring and winter in the Saharan male lizard Uromastyx acanthinura (Sauria, Agamidae), with special reference to body water economy. , 2020, Tissue & cell.

[2]  J. Antunes-Rodrigues,et al.  Sex and age dependent differences in the hormone and drinking responses to water deprivation. , 2020, American journal of physiology. Regulatory, integrative and comparative physiology.

[3]  J. Antunes-Rodrigues,et al.  Imbalance of Pro- and Anti-Angiogenic Factors Due to Maternal Vitamin D Deficiency Causes Renal Microvasculature Alterations Affecting the Adult Kidney Function , 2019, Nutrients.

[4]  D. Begg,et al.  Disturbances of thirst and fluid balance associated with aging , 2017, Physiology & Behavior.

[5]  M. Yanagita,et al.  Resident fibroblasts in the kidney: a major driver of fibrosis and inflammation , 2017, Inflammation and regeneration.

[6]  Michael Gekle,et al.  Kidney and aging — A narrative review , 2017, Experimental Gerontology.

[7]  L. Hooper.  Why, Oh Why, Are So Many Older Adults Not Drinking Enough Fluid? , 2016, Journal of the Academy of Nutrition and Dietetics.

[8]  N. Stachenfeld.  Hormonal Changes During Menopause and the Impact on Fluid Regulation , 2014, Reproductive Sciences.

[9]  J. Tuazon,et al.  Anatomic and physiologic changes of the aging kidney. , 2013, Clinics in geriatric medicine.

[10]  H. Yoon,et al.  Effect of estradiol on the expression of renal sodium transporters in rats , 2013, Climacteric : the journal of the International Menopause Society.

[11]  B. Lanske,et al.  FGF23 regulates renal sodium handling and blood pressure , 2012, EMBO molecular medicine.

[12]  I. Pawluczyk,et al.  Effect of angiotensin type 2 receptor over-expression on the rat mesangial cell fibrotic phenotype: effect of gender , 2012, Journal of the renin-angiotensin-aldosterone system : JRAAS.

[13]  S. Anderson,et al.  The aging kidney: physiological changes. , 2010, Advances in chronic kidney disease.

[14]  S. Bachmann,et al.  Short-term stimulation of the thiazide-sensitive Na+-Cl- cotransporter by vasopressin involves phosphorylation and membrane translocation. , 2010, American journal of physiology. Renal physiology.

[15]  J. Sands.  Urinary concentration and dilution in the aging kidney. , 2009, Seminars in nephrology.

[16]  J. Verbalis,et al.  Renal ENaC subunit, Na-K-2Cl and Na-Cl cotransporter abundances in aged, water-restricted F344 x Brown Norway rats. , 2006, Kidney international.

[17]  I. Armando,et al.  Estrogen upregulates renal angiotensin II AT1 and AT2 receptors in the rat , 2005, Regulatory Peptides.

[18]  H. Matsubara,et al.  Overexpression of angiotensin type 2 receptor ameliorates glomerular injury in a mouse remnant kidney model. , 2004, American journal of physiology. Renal physiology.

[19]  M. Kleinfeld,et al.  Hypernatremia in the aging: causes, manifestations, and outcome. , 1995, Journal of the National Medical Association.

[20]  S. Fischer,et al.  Adaptation of the rat kidney to altered water intake and urine concentration , 1988, Pflügers Archiv.

[21]  W. Kriz,et al.  Renal Physiology , 1986, Springer New York.

[22]  B. Kasiske,et al.  The influence of age, sex, race, and body habitus on kidney weight in humans. , 1986, Archives of pathology & laboratory medicine.

[23]  Lindeman Rd.  The aging kidney. , 1986, Comprehensive therapy.

[24]  P. Wasserman,et al.  Changes in sizes and distensibility of the aging kidney. , 1981, The British journal of radiology.

[25]  K. Baskoy,et al.  Effect of testosterone replacement therapy on vitamin D and FGF-23 levels in congenital hypogonadism. , 2017, Endokrynologia Polska.

[26]  A. Rule,et al.  Structural and Functional Changes With the Aging Kidney. , 2016, Advances in chronic kidney disease.

[27]  柏木 稔.  Locally Activated Renin-Angiotensin System Associated with TGF-β1 as a Major Factor for Renal Injury Induced by Chronic Inhibition of Nitric Oxide Synthase in Rats , 2001 .

[28]  D. Rodríguez‐Puyol,et al.  Age-related progressive renal fibrosis in rats and its prevention with ACE inhibitors and taurine. , 2000, American journal of physiology. Renal physiology.

[29]  Diego Rodrfguez-Puyol,et al.  The aging kidney. , 1998, Kidney international.