Long-term cryopreservation of sperm of Japanese eel

After cryopreservation in a diluent (D#5-D), containing 76.2 mM NaHCO 3 , 137-0 mM NaCI, 1.4% soya lecithin and 10% dimethyl sulphoxide, for 30-180 days, Japanese eel Anguilla japonica sperm was 61.4-70.6% motile compared with fresh sperm. Cryopreserved sperm was used for fertilizing three batches of eggs and hatchability ranged from 20.7 to 69.1%. These results indicate that D#5-D is an useful diluent for sperm cryopreservation in Japanese eel.

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