Sperm DNA fragmentation testing reveals the overall quality of a semen sample

We read with interest the well expressed commentary by Dr. Hallak (1) and he provided us with greater insight into different sperm DNA fragmentation (SDF) tests. Dr. Hallak highlighted an interesting point that “ having a DNA fragmentation index (DFI) of ” (1,2). We think that it is one of the central concepts in understanding the different aspects of current SDF tests.

[1]  S. Esteves,et al.  Use of testicular sperm for intracytoplasmic sperm injection in men with high sperm DNA fragmentation: a SWOT analysis , 2017, Asian journal of andrology.

[2]  Sajal Gupta,et al.  Inter‐and Intra‐Laboratory Standardization of TUNEL Assay for Assessment of Sperm DNA Fragmentation , 2017, Current protocols in toxicology.

[3]  J. Hallak Utility of sperm DNA fragmentation testing in different clinical scenarios of male reproductive abnormalities and its influence in natural and assisted reproduction , 2017, Translational andrology and urology.

[4]  U. Schmidt,et al.  Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis , 2016, Andrology.

[5]  A. Agarwal,et al.  Should we evaluate and treat sperm DNA fragmentation? , 2016, Current opinion in obstetrics & gynecology.

[6]  S. Esteves,et al.  Comparison of reproductive outcome in oligozoospermic men with high sperm DNA fragmentation undergoing intracytoplasmic sperm injection with ejaculated and testicular sperm. , 2015, Fertility and sterility.

[7]  S. Esteves,et al.  Unpacking the mysteries of sperm DNA fragmentation Ten frequently asked questions , 2015 .

[8]  K. Jarvi,et al.  A comparison of ejaculated and testicular spermatozoa aneuploidy rates in patients with high sperm DNA damage , 2012, Systems biology in reproductive medicine.

[9]  M. Meseguer,et al.  Effect of sperm DNA fragmentation on pregnancy outcome depends on oocyte quality. , 2011, Fertility and sterility.

[10]  P. Bray-Ward,et al.  Sperm selection for ICSI: shape properties do not predict the absence or presence of numerical chromosomal aberrations. , 2004, Human reproduction.

[11]  R. Aitken,et al.  The human spermatozoon--not waving but drowning. , 2003, Advances in experimental medicine and biology.

[12]  Jens Peter Bonde,et al.  Sperm chromatin damage impairs human fertility , 2000 .

[13]  J. Bonde,et al.  Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team. , 2000, Fertility and sterility.

[14]  T. Kirchhoff,et al.  DNA polymerase lambda (Pol lambda), a novel eukaryotic DNA polymerase with a potential role in meiosis. , 2000, Journal of molecular biology.

[15]  S. Ng,et al.  Fertilizing ability of DNA-damaged spermatozoa. , 1999, The Journal of experimental zoology.

[16]  M. Melamed,et al.  Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometry. , 1975, Experimental cell research.