Polymorphism analysis of four canine MHC class I genes.

We have studied the variability of four structurally complete dog leukocyte antigen (DLA) class I genes, termed DLA-12, -88, -79 and -64, in a population of mixed breed, unrelated dogs. The human HLA and canine DLA loci share a high degree of similarity in terms of gene structure. This analysis focused on the first three exons of each of four complete canine genes. Exons two and three are the major source of polymorphism in the corresponding human genes. In this analysis, DLA-88 was found to be significantly more polymorphic than the other three genes, with 44 distinct alleles observed among 63 mixed breed, unrelated dogs. The remaining genes had between one and four alleles when examined in 25 dogs. This work was carried out as part of an effort to develop an MHC typing system for the dog, which is critical to the further development of preclinical studies of hematopoietic stem cell and solid organ transplantation in the canine model.

[1]  R. Storb,et al.  Molecular analysis of six dog leukocyte antigen class I sequences including three complete genes, two truncated genes and one full-length processed gene. , 1997, Tissue antigens.

[2]  D. Geraghty,et al.  Structure and expression of a divergent canine class I gene. , 1995, Journal of immunology.

[3]  Patrick Dowd,et al.  Confirmation of BRCA1 by analysis of germline mutations linked to breast and ovarian cancer in ten families , 1994, Nature Genetics.

[4]  J. Rodgers,et al.  Antigen presentation by major histocompatibility complex class I-B molecules. , 1994, Annual review of immunology.

[5]  D. Geraghty.  Structure of the HLA class I region and expression of its resident genes. , 1993, Current opinion in immunology.

[6]  M A Nowak,et al.  The optimal number of major histocompatibility complex molecules in an individual. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[7]  R. Storb,et al.  Canine models of bone marrow transplantation. , 1990, Laboratory animal science.

[8]  A. Townsend,et al.  Antigen recognition by class I-restricted T lymphocytes. , 1989, Annual review of immunology.

[9]  T. Kunkel,et al.  Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. , 1988, Biochemistry.

[10]  M. A. Saper,et al.  The foreign antigen binding site and T cell recognition regions of class I histocompatibility antigens , 1987, Nature.

[11]  A Sette,et al.  Immunological self, nonself discrimination. , 1987, Science.

[12]  H. Deeg,et al.  Joint report of the Third International Workshop on Canine Immunogenetics. I. Analysis of homozygous typing cells. , 1986, Transplantation.

[13]  W. Rutter,et al.  Polymorphic DNA region adjacent to the 5' end of the human insulin gene. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[14]  M. Dorf,et al.  The Major Histocompatibility System of the Dog , 1977 .

[15]  R. Storb,et al.  MARROW GRAFTS BY COMBINED MARROW AND LEUKOCYTE INFUSIONS IN UNRELATED DOGS SELECTED BY HISTOCOMPATIBILITY TYPING , 1968, Transplantation.

[16]  R. Storb,et al.  CYTOTOXIC TYPING ANTISERA FOR MARROW GRAFTING IN LITTERMATE DOGS , 1968, Transplantation.