Transforming growth factor-beta 1 stimulates chondrogenesis and inhibits osteogenesis in high density culture of periosteum-derived cells.

Chick periosteum-derived mesenchymal cells have been reported to exhibit both osteogenic and chondrogenic potentials in high cell density culture conditions. Using this culture system, the effects of transforming growth factor-beta (TGF beta) on proliferation and differentiation of periosteal mesenchymal cells were studied. Supplementation with TGF beta 1 at doses of 0.3-1.0 ng/ml shortened the time course of chondrogenesis and increased the amount of cartilage formed in the lower part of the culture. On the other hand, the amount of bone formed in the upper part of the culture decreased with TGF beta treatment, whereas the time course of osteogenesis remained unaffected.

[1]  K. Ono,et al.  Collagen gene expression during chondrogenesis from chick periosteum‐derived cells , 1992, FEBS letters.

[2]  S. Bruder,et al.  In vitro differentiation of bone and hypertrophic cartilage from periosteal-derived cells. , 1991, Experimental cell research.

[3]  M E Bolander,et al.  Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur , 1990, The Journal of cell biology.

[4]  W. Kulyk,et al.  Promotion of embryonic chick limb cartilage differentiation by transforming growth factor-beta. , 1989, Developmental biology.

[5]  E. Canalis,et al.  Skeletal tissue and transforming growth factor β , 1988, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[6]  D. Rosen,et al.  Transforming growth factor‐beta modulates the expression of osteoblast and chondroblast phenotypes in vitro , 1988, Journal of cellular physiology.

[7]  G. Rodan,et al.  Type β transforming growth factor (TGFβ) regulation of alkaline phosphatase expression and other phenotype‐related mRNAs in osteoblastic rat osteosarcoma cells , 1987 .

[8]  J. Massagué,et al.  Human platelet-derived transforming growth factor-beta stimulates parameters of bone growth in fetal rat calvariae. , 1986, Endocrinology.

[9]  J. McPherson,et al.  Cartilage-inducing factor-A. Apparent identity to transforming growth factor-beta. , 1986, The Journal of biological chemistry.

[10]  D. Rosen,et al.  Purification and characterization of two cartilage-inducing factors from bovine demineralized bone. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[11]  N. Maeda,et al.  Appearance of distinct types of proteoglycan in a well-defined temporal and spatial pattern during early cartilage formation in the chick limb. , 1984, Developmental biology.

[12]  A. Caplan.  Effects of the nicotinamide-sensitive teratogen3-acetylpyridine on chick limb cells in culture. , 1970, Experimental cell research.

[13]  R Umansky,et al.  The effect of cell population density on the developmental fate of reaggregating mouse limb bud mesenchyme. , 1966, Developmental biology.

[14]  A. Ham.  A HISTOLOGICAL STUDY OF THE EARLY PHASES OF BONE REPAIR , 1930 .

[15]  A. Caplan,et al.  Morphology of bone development and bone remodeling in embryonic chick limbs. , 1986, Bone.