Enoate Reductases of Clostridia

The enr genes specifying enoate reductases of Clostridium tyrobutyricum andClostridium thermoaceticum were cloned and sequenced. Sequence comparison shows that enoate reductases are similar to a family of flavoproteins comprising 2,4-dienoyl-coenzyme A reductase from Escherichia coli and old yellow enzyme from yeast. TheC. thermoaceticum enr gene product was expressed in recombinant Escherichia coli cells growing under anaerobic conditions. The recombinant enzyme was purified and characterized.

[1]  V. Massey,et al.  Conversion of a Flavoprotein Reductase to a Desaturase by Manipulation of the Flavin Redox Potential , 1999 .

[2]  R. Fleischmann,et al.  The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus , 1997, Nature.

[3]  Song-Yu Yang,et al.  Cloning and expression of the fadH gene and characterization of the gene product 2,4-dienoyl coenzyme A reductase from Escherichia coli. , 1997, European journal of biochemistry.

[4]  H. Simon,et al.  Chiral synthons by selective redox reactions catalysed by hitherto unknown enzymes present in resting microbial cells , 1997 .

[5]  M. Teixeira,et al.  EPR and Mössbauer Spectroscopic Studies on Enoate Reductase* , 1996, The Journal of Biological Chemistry.

[6]  N. Scrutton,et al.  The primary structure of Hyphomicrobium X dimethylamine dehydrogenase. Relationship to trimethylamine dehydrogenase and implications for substrate recognition. , 1995, European journal of biochemistry.

[7]  S. Chakraborty,et al.  Old Yellow enzyme: aromatization of cyclic enones and the mechanism of a novel dismutation reaction. , 1995, Biochemistry.

[8]  J. Hiltunen,et al.  Isolation and characterization of cDNA for human 120 kDa mitochondrial 2,4-dienoyl-coenzyme A reductase. , 1994, The Biochemical journal.

[9]  M. Klingbeil,et al.  Characterization of cDNA clones for the 2-methyl branched-chain enoyl-CoA reductase. An enzyme involved in branched-chain fatty acid synthesis in anaerobic mitochondria of the parasitic nematode Ascaris suum. , 1993, The Journal of biological chemistry.

[10]  R. Scopes,et al.  Cloning, sequencing and expression of the gene encoding NADH oxidase from the extreme anaerobic thermophile Thermoanaerobium brockii. , 1993, Biochimica et biophysica acta.

[11]  S. F. Baron,et al.  Characterization of the baiH gene encoding a bile acid-inducible NADH:flavin oxidoreductase from Eubacterium sp. strain VPI 12708 , 1993, Journal of bacteriology.

[12]  S. White,et al.  Correlation of x-ray deduced and experimental amino acid sequences of trimethylamine dehydrogenase. , 1994, The Journal of biological chemistry.

[13]  N. Scrutton,et al.  Trimethylamine dehydrogenase of bacterium W3A1 Molecular cloning, sequence determination and over‐expression of the gene , 1992, FEBS letters.

[14]  D. Thiele,et al.  The cloning and expression of a gene encoding Old Yellow Enzyme from Saccharomyces carlsbergensis. , 1991, The Journal of biological chemistry.

[15]  David A. Mead,et al.  A Universal Method for the Direct Cloning of PCR Amplified Nucleic Acid , 1991, Bio/Technology.

[16]  F. Mȕller.  Chemistry and Biochemistry of Flavoenzymes: Volume I , 1991 .

[17]  P. Hylemon,et al.  Cloning and sequencing of a bile acid-inducible operon from Eubacterium sp. strain VPI 12708 , 1990, Journal of bacteriology.

[18]  E. L. Harris,et al.  Protein purification applications : a practical approach , 1990 .

[19]  M. Young,et al.  Recent advances in the genetics of the clostridia. , 1989, FEMS microbiology reviews.

[20]  H. Simon,et al.  Carboxylic acid reductase: a new tungsten enzyme catalyses the reduction of non-activated carboxylic acids to aldehydes. , 1989, European journal of biochemistry.

[21]  W. Boland,et al.  Synthesis of Chiral 12‐Phenyl(2H)dodecanoic Acids: Useful metabolic probes for the biosynthesis of 1‐alkenes from fatty acids , 1989 .

[22]  G. Krause,et al.  Design and Applications of Sensitive Enzyme Immunoassays Specific for Clostridial Enoate Reductases , 1989, Zeitschrift fur Naturforschung. C, Journal of biosciences.

[23]  P. Model,et al.  Sequence of thioredoxin reductase from Escherichia coli. Relationship to other flavoprotein disulfide oxidoreductases. , 1988, The Journal of biological chemistry.

[24]  H. Simon,et al.  Reductions of 2-enals, dehydrogenation of saturated aldehydes and their racemisation. , 1988, Biological Chemistry Hoppe-Seyler.

[25]  H. Simon,et al.  Electro-enzymic viologen-mediated stereospecific reduction of 2-enoates with free and immobilized enoate reductase on cellulose filters or modified carbon electrodes , 1987 .

[26]  H. Simon,et al.  Stereospecific Reductions with Hydrogen Gas, Modified Metal Catalysts, Methyl Viologen and Enzymes or Microorganisms , 1986 .

[27]  J. Bader,et al.  Chiral Compounds Synthesized by Biocatalytic Reductions [New Synthetic Methods (51)] , 1985 .

[28]  A. Bacher,et al.  Structure of enoate reductase from a Clostridium tyrobutyricum (C. spec. La1). , 1985, Biological chemistry Hoppe-Seyler.

[29]  W. Kunau,et al.  2,4-Dienoyl coenzyme A reductases from bovine liver and Escherichia coli. Comparison of properties. , 1984, The Journal of biological chemistry.

[30]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[31]  H. Simon,et al.  On the kinetics and mechanism of enoate reductase. , 1982, Hoppe-Seyler's Zeitschrift fur physiologische Chemie.

[32]  P. Rauschenbach,et al.  On a hitherto unknown fermentation path of several amino acids by proteolytic clostridia , 1982, FEBS letters.

[33]  Susan E. Brown,et al.  Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis , 1982, Journal of bacteriology.

[34]  R. Schirmer,et al.  Glutathione reductase from human erythrocytes. The sequences of the NADPH domain and of the interface domain. , 1982, European journal of biochemistry.

[35]  R. Schirmer,et al.  Glutathione reductase from human erythrocytes: amino-acid sequence of the structurally known FAD-binding domain. , 1981, European journal of biochemistry.

[36]  D. Northcote,et al.  Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. , 1981, Analytical biochemistry.

[37]  J. Bader,et al.  The reduction of allyl alcohols by Clostridium species is catalyzed by the combined action of alcohol dehydrogenase and enoate reductase. , 1981, Hoppe-Seyler's Zeitschrift fur physiologische Chemie.

[38]  W. Tischer,et al.  Occurrence and the possible physiological role of 2‐enoate reductases , 1980, FEBS letters.

[39]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[40]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[41]  M. Villarejo,et al.  Construction and Properties of Escherichia coli Strains Exhibiting α-Complementation of β-Galactosidase Fragments In Vivo , 1972, Journal of bacteriology.

[42]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.