A solid medium for visual demonstration of coagulase production by Staphylococcus aureus.

The tube test for free coagulase is the most reliable methed for the distinction between Staph. aureus and Staph. albus in the clinical laboratory, since the whole plasma-containing solid media (Penfold, 1944; Lack, 1957; Esber and Faulconer, 1959) show false-positive reactions with coagulase-negative staphylococci (Williams and Harper, 1946; Klemperer and Haughton, 1957; Lotter and Horstman, 1967). These non-specific reactions appear practically eliminated in the medium of Klemperer and Haughton (1957) because of its low plasma content (3 %). In practice this medium has the inconvenience that any new batch of plates must be prepared with fresh active plasma. As the clotting activity of coagulase is based on its reaction with a coagulasereacting factor in plasma, which is identical with prothrombin (Tager, 1956; Soulier and Prou, Wartelle, 1967), a prothrombin preparation could also be used. The object of the present paper is to describe a solid medium for the detection of coagulase production using a stable human prothrombin preparation.

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