Presence of kdr and s-kdr resistance in Musca domestica populations collected in Piacenza province (Northern Italy).

Pyrethroid insecticides combine a high efficacy against insects with a low toxicity towards warm-blooded vertebrates. For this reason they are largely used to control housefly infestations. The efficacy of these products is affected by insecticide resistance mechanisms. In particular, some mutations in the sodium channel coding sequence are responsible for target-site resistance to pyrethroid insecticides. This work presents a new molecular approach based on allele-specific PCRs to point out and to characterise this resistance mechanism in two M. domestica populations (PNT and TRV) collected near Piacenza (Northern Italy). The presence of different kdr and s-kdr genotypes was assessed for population PNT whilst kdr only was detected in population TRV. Dose-response bioassays evidenced quite high resistance factors, expecially for population PNT. This is in line with the kdr and s-kdr frequencies observed in the assayed populations.

[1]  Y. Nomura,et al.  Molecular biology of insect sodium channels and pyrethroid resistance. , 2014, Insect biochemistry and molecular biology.

[2]  M. Panini,et al.  Detecting the presence of target-site resistance to neonicotinoids and pyrethroids in Italian populations of Myzus persicae. , 2014, Pest management science.

[3]  Yuzhe Du,et al.  Diversity and Convergence of Sodium Channel Mutations Involved in Resistance to Pyrethroids. , 2013, Pesticide biochemistry and physiology.

[4]  Seán G. Brady,et al.  Multiple Origins of kdr-type Resistance in the House Fly, Musca domestica , 2012, PloS one.

[5]  Jing Pan,et al.  PCR-RFLP methods for detection of insecticide resistance-associated mutations in the house fly (Musca domestica) , 2012 .

[6]  M. Leis,et al.  Preliminary evaluation of insecticide resistance in a strain of Musca domestica (Diptera: Muscidae) from an intensive chicken farm of Northern Italy , 2011, Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes.

[7]  D. M. Soderlund,et al.  Molecular mechanisms of pyrethroid insecticide neurotoxicity: recent advances , 2011, Archives of Toxicology.

[8]  R. Hamm,et al.  Dynamics of insecticide resistance alleles in house fly populations from New York and Florida. , 2007, Insect biochemistry and molecular biology.

[9]  K. Pfeffer,et al.  Pilot study on synanthropic flies (e.g. Musca, Sarcophaga, Calliphora, Fannia, Lucilia, Stomoxys) as vectors of pathogenic microorganisms , 2007, Parasitology Research.

[10]  P. Usherwood,et al.  DDT, pyrethrins, pyrethroids and insect sodium channels , 2007, IUBMB life.

[11]  B. Lazzaro,et al.  Frequencies of the pyrethroid resistance alleles of Vssc1 and CYP6D1 in house flies from the eastern United States , 2006, Insect molecular biology.

[12]  T. Zhao,et al.  Survey of Deltamethrin Resistance in House Flies (Musca domestica) from Urban Garbage Dumps in Northern China , 2006 .

[13]  M. Moeschberger,et al.  Spread of Musca domestica (Diptera: Muscidae), from Two Caged Layer Facilities to Neighboring Residences in Rural Ohio , 2005, Journal of medical entomology.

[14]  A. Devonshire,et al.  Identification of mutations in the houseflypara-type sodium channel gene associated with knockdown resistance (kdr) to pyrethroid insecticides , 1996, Molecular and General Genetics MGG.

[15]  M. Kristensen,et al.  Frequency of kdr Gene in House Fly Field Populations: Correlation of Pyrethroid Resistance and kdr Frequency , 2004, Journal of economic entomology.

[16]  D. M. Soderlund,et al.  The molecular biology of knockdown resistance to pyrethroid insecticides. , 2003, Insect biochemistry and molecular biology.

[17]  R. Wilkins,et al.  Studies on some enzymes involved in insecticide resistance in fenitrothion‐resistant and ‐susceptible strains of Musca domestica L. (Dipt, Muscidae) , 2002 .

[18]  Nannan Liu,et al.  Metabolic detoxication and the kdr mutation in pyrethroid resistant house flies, Musca domestica (L.) , 2002 .

[19]  S. Kasai,et al.  Genetics and mechanisms of permethrin resistance in the YPER strain of house fly , 2002 .

[20]  D. Church,et al.  Spidey: a tool for mRNA-to-genomic alignments. , 2001, Genome research.

[21]  J. Howard.  Nuisance flies around a landfill: Patterns of abundance and distribution , 2001, Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA.

[22]  R. Gilman,et al.  The role of non-biting flies in the epidemiology of human infectious diseases. , 2001, Microbes and infection.

[23]  D. M. Soderlund,et al.  Mutations in the house fly Vssc1 sodium channel gene associated with super-kdr resistance abolish the pyrethroid sensitivity of Vssc1/tipE sodium channels expressed in Xenopus oocytes. , 1999, Insect biochemistry and molecular biology.

[24]  M. Trentini,et al.  Monitoraggio delle mosche in tre caseifici della provincia di Bologna , 1999 .

[25]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[26]  A. Belcari,et al.  Alcune osservazioni sulla composizione quali-quantitativa della ditterofauna presente in stalle di bovini per la produzione del latte , 1989 .

[27]  W. S. Abbott,et al.  A method of computing the effectiveness of an insecticide. 1925. , 1925, Journal of the American Mosquito Control Association.