Extraction of starch and proteins from the tubers of large-grain Cyperus esculentus L

WENBING MENG, LIDAO BAO, CHANGQING LI, XIANHUA REN, QIN SI, JIANJUN REN*, AGULA B.* 1. Department of Obstetrics and Gynecology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, P. R. China 2. Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, P. R. China 3. Department of Geriatrics, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, P. R. China 4. Department of General Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, P. R. China 5. College of Traditional Mongolia Medicine, Inner Mongolia Medical University, Hohhot, 010110, P. R. China † Wenbing Meng and Lidao Bao contributed equally to this study. *Corresponding author: Jianjun Ren: Email: renjianjun237@163.com Agula B.: Email: bagulaimmu@163.com Abstract The contents of starch in the tubers of Cyperus esculentus L. were determined by the iodine method, and those of proteins were determined by the Coomassie brilliant blue G-250 method. Starch and proteins were simultaneously extracted by the lime water immersion method, and the effects of material-liquid ratio, immersion solution pH value, immersion time and supernatant pH value on the extraction efficiencies were studied. The optimal parameters were obtained by orthogonal experiments. The results show that the contents of starch and protein in large-grain C. esculentus are 35.4% and 6.3%, respectively. The optimal extraction conditions for starch include: immersion solution pH = 11.0, material-liquid ratio = 1:4 (w:v), immersion time = 70 min. The optimal extraction conditions for proteins include: immersion solution pH = 10.5, material-liquid ratio = 1:5 (w:v), immersion time = 50 min. Proteins were precipitated from the supernatant at pH 5.5.

[1]  Zhiyan Ren,et al.  The in vitro and in vivo antioxidant properties of Cyperus esculentus oil from Xinjiang, China. , 2013, Journal of the science of food and agriculture.

[2]  M. Emeje,et al.  Physicochemical and Binder Properties of Starch Obtained from Cyperus esculentus , 2012, AAPS PharmSciTech.

[3]  F. Cui,et al.  [Expression of matrix metalloproteinases and inhibitor on the cornea tissue in rabbit after implantation of modified titanium skirt for keratoprosthesis]. , 2012, [Zhonghua yan ke za zhi] Chinese journal of ophthalmology.

[4]  K. Seetharaman,et al.  Quantification of total iodine in intact granular starches of different botanical origin exposed to iodine vapor at various water activities. , 2011, Carbohydrate research.

[5]  S. Stymne,et al.  Characterization of oil and starch accumulation in tubers of Cyperus esculentus var. sativus (Cyperaceae): A novel model system to study oil reserves in nonseed tissues. , 2010, American journal of botany.

[6]  E. Sendra,et al.  Preparation of dietary fiber powder from tiger nut (Cyperus esculentus) milk ("Horchata") byproducts and its physicochemical properties. , 2009, Journal of agricultural and food chemistry.

[7]  N. Prakash,et al.  Phytochemical observation and antibacterial activity of Cyperus esculentus L. , 2009, Ancient science of life.

[8]  K. Seebold,et al.  Effect of application timing and method on efficacy and phytotoxicity of 1,3-D, chloropicrin and metam-sodium combinations in squash plasticulture. , 2008, Pest management science.

[9]  M. Millson,et al.  Amino Acid, Mineral and Fatty Acid Content of Pumpkin Seeds (Cucurbita spp) and Cyperus esculentus Nuts in the Republic of Niger , 2006, Plant foods for human nutrition.

[10]  Y. Cha,et al.  A Quantitative Nitroblue Tetrazolium Assay for Determining Intracellular Superoxide Anion Production in Phagocytic Cells , 2006, Journal of immunoassay & immunochemistry.

[11]  L. O. Adebajo.  Survey of aflatoxins and ochratoxin A in stored tubers ofCyperus esculentus L. , 1993, Mycopathologia.

[12]  Wei Sun,et al.  Linear sweep voltammetric determination of protein based on its interaction with Alizarin Red S. , 2002, Talanta.

[13]  J. T. Barminas,et al.  A preliminary investigation into the biofuel characteristics of tigernut (Cyperus esculentus) oil. , 2001, Bioresource technology.

[14]  M. Parker,et al.  Esterified phenolics of the cell walls of chufa (Cyperus esculentusL. ) tubers and their role in texture. , 2000, Journal of agricultural and food chemistry.

[15]  E. Stoller,et al.  Differential cold tolerance, starch, sugar, protein, and lipid of yellow and purple nutsedge tubers. , 1975, Plant physiology.