Detection of Brucella canis in a dog in Italy.

Brucella spp. is a worldwide zoonotic pathogen. Infection by Brucella canis in dogs is endemic in the Southern USA and in Central and South America, but it appears sporadically in other parts of the world, including Europe. Tissue samples from a dog with chronic prostatitis, discospondylitis and locomotor problems were subjected to clinical and laboratory examinations. B. canis was detected by PCR in biological fluids and tissues of the animal, while antibodies to B. canis were found in the serum, providing additional strong evidence for the circulation of B. canis in Italy.

[1]  S. Vasconcellos,et al.  Comparison of agar gel immunodiffusion test, rapid slide agglutination test, microbiological culture and PCR for the diagnosis of canine brucellosis. , 2009, Research in veterinary science.

[2]  F. Ciuchini,et al.  Brucella melitensis B115‐based complement fixation test to detect antibodies induced by Brucella rough strains , 2008, Journal of applied microbiology.

[3]  S. Vasconcellos,et al.  A polymerase chain reaction for detection of Brucella canis in vaginal swabs of naturally infected bitches. , 2007, Theriogenology.

[4]  A. Yamada,et al.  Simultaneous detection of the genus Brucella by combinatorial PCR. , 2007, Japanese journal of infectious diseases.

[5]  S. Vasconcellos,et al.  A polymerase chain reaction for the detection of Brucella canis in semen of naturally infected dogs. , 2007, Theriogenology.

[6]  G. Escobar,et al.  Unusual clinical presentation of brucellosis caused by Brucella canis. , 2005, Journal of medical microbiology.

[7]  W. Probert,et al.  Real-Time Multiplex PCR Assay for Detection of Brucella spp., B. abortus, and B. melitensis , 2004, Journal of Clinical Microbiology.

[8]  M. Wanke.  Canine brucellosis. , 2004, Animal reproduction science.

[9]  L. Nimri.  Diagnosis of recent and relapsed cases of human brucellosis by PCR assay , 2003, BMC infectious diseases.

[10]  F. Fratini,et al.  Serological diagnosis of brucellosis caused by Brucella canis. , 2003, The new microbiologica.

[11]  P. Kutzer,et al.  [Canine brucellosis--a case report]. , 2003, Berliner und Munchener tierarztliche Wochenschrift.

[12]  D. O’Callaghan,et al.  The Brucella genome at the beginning of the post-genomic era. , 2002, Veterinary microbiology.

[13]  B. Bricker,et al.  PCR as a diagnostic tool for brucellosis. , 2002, Veterinary microbiology.

[14]  J. Dunne,et al.  Canine brucellosis in a dog imported into the UK. , 2002, The Veterinary record.

[15]  M. Queipo-Ortuño,et al.  Diagnostic Yield of a PCR Assay in Focal Complications of Brucellosis , 1999, Journal of Clinical Microbiology.

[16]  P. Ocón,et al.  Rapid diagnosis of human brucellosis by peripheral-blood PCR assay , 1997, Journal of clinical microbiology.

[17]  S. Shin,et al.  Canine brucellosis: a diagnostician's dilemma. , 1996, Seminars in veterinary medicine and surgery.

[18]  V. Dutra,et al.  Brucelose canina: relato de caso , 1996 .

[19]  C. Gamazo,et al.  Specific detection of Brucella DNA by PCR , 1995, Journal of clinical microbiology.

[20]  B. Drasar,et al.  Detection of Brucella melitensis and Brucella abortus by DNA amplification. , 1992, The Journal of tropical medicine and hygiene.

[21]  L. Carmichael,et al.  A rapid slide agglutination test for the serodiagnosis of Brucella canis infection that employs a variant (M-) organism as antigen. , 1987, The Cornell veterinarian.

[22]  L. Carmichael,et al.  Improved rapid slide agglutination test for presumptive diagnosis of canine brucellosis , 1982, Journal of clinical microbiology.

[23]  R. Flores-Castro,et al.  A serological and bacteriological survey of canine brucellosis in Mexico. , 1976, The Cornell veterinarian.

[24]  L. Carmichael,et al.  Canine brucellosis: the clinical disease, pathogenesis, and immune response. , 1970, Journal of the American Veterinary Medical Association.