Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae

Eleven primer pairs were developed for the identification of Neisseria gonorrhoeae. The sensitivity and specificity of these primers were evaluated by Real Time (RT)-PCR melt curve analyses with DNA from 145 N. gonorrhoeae isolates and 40 other Neisseria or non-Neisseria species. Three primer pairs were further evaluated in a hydrogel-based RT-PCR detection platform, using DNA extracted from 50 N. gonorrhoeae cultures. We observed 100% sensitivity and specificity in the hydrogel assay, confirming its potential as a point-of-care test (POCT) for N. gonorrhoeae diagnosis.

[1]  R. Peeling,et al.  Performance and operational characteristics of point-of-care tests for the diagnosis of urogenital gonococcal infections , 2017, Sexually Transmitted Infections.

[2]  J. Klausner,et al.  Prevalence and treatment outcomes of routine Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis testing during antenatal care, Gaborone, Botswana , 2017, Sexually Transmitted Infections.

[3]  D. Speers,et al.  Detection of Neisseria gonorrhoeae and Chlamydia trachomatis from pooled rectal, pharyngeal and urine specimens in men who have sex with men , 2017, Sexually Transmitted Infections.

[4]  D. Taylor-Robinson,et al.  Diagnosis of some genital-tract infections: part 1. An historical perspective , 2017, International journal of STD & AIDS.

[5]  A. Nardone,et al.  Impact of deploying multiple point-of-care tests with a ‘sample first’ approach on a sexual health clinical care pathway. A service evaluation , 2017, Sexually Transmitted Infections.

[6]  C. Gaydos,et al.  Stability Studies on Dry Swabs and Wet Mailed Swabs for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Aptima Assays , 2017, Journal of Clinical Microbiology.

[7]  F. Vazquez,et al.  Rapid diagnosis of sexually transmitted infections. , 2017, Enfermedades infecciosas y microbiologia clinica.

[8]  J. Kaldor,et al.  A novel point-of-care testing strategy for sexually transmitted infections among pregnant women in high-burden settings: results of a feasibility study in Papua New Guinea , 2016, BMC Infectious Diseases.

[9]  R M A Graham,et al.  Epidemiological typing of Neisseria gonorrhoeae and detection of markers associated with antimicrobial resistance directly from urine samples using next generation sequencing , 2016, Sexually Transmitted Infections.

[10]  Marleen Temmerman,et al.  Global Estimates of the Prevalence and Incidence of Four Curable Sexually Transmitted Infections in 2012 Based on Systematic Review and Global Reporting , 2015, PloS one.

[11]  C. Bébéar,et al.  Comparison of three real-time PCR assays for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in young pregnant women. , 2015, Diagnostic microbiology and infectious disease.

[12]  Nitika Pant Pai,et al.  Point-of-Care Diagnostic Testing in Global Health: What Is the Point?: The main goal of such testing is to inform caregivers in ways that lead rapidly to their starting correct treatments for patients , 2015 .

[13]  J. Dillon,et al.  Antibiotic Resistance in Neisseria gonorrhoeae: Will Infections be Untreatable in the Future? , 2015 .

[14]  T. Read,et al.  Using Crude Whole-Genome Assemblies of Neisseria gonorrhoeae as a Platform for Strain Analysis: Clonal Spread of Gonorrhea Infection in Saskatchewan, Canada , 2014, Journal of Clinical Microbiology.

[15]  A. Mbonye,et al.  A lab-on-chip for malaria diagnosis and surveillance , 2014, Malaria Journal.

[16]  Xiangsheng Chen,et al.  Evaluation of Abbott RealTime CT/NG Assay for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Cervical Swabs from Female Sex Workers in China , 2014, PloS one.

[17]  M. Unemo,et al.  Analytical Evaluation of GeneXpert CT/NG, the First Genetic Point-of-Care Assay for Simultaneous Detection of Neisseria gonorrhoeae and Chlamydia trachomatis , 2013, Journal of Clinical Microbiology.

[18]  C. Gaydos,et al.  Performance of the Cepheid CT/NG Xpert Rapid PCR Test for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae , 2013, Journal of Clinical Microbiology.

[19]  M. Unemo,et al.  Emergence of multidrug-resistant, extensively drug-resistant and untreatable gonorrhea. , 2012, Future microbiology.

[20]  J. Kaldor,et al.  Point-of-care tests for the diagnosis of Neisseria gonorrhoeae infection: a systematic review of operational and performance characteristics , 2012, Sexually Transmitted Infections.

[21]  Jian Ye,et al.  Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction , 2012, BMC Bioinformatics.

[22]  H. John Crabtree,et al.  A miniaturized and integrated gel post platform for multiparameter PCR detection of herpes simplex viruses from raw genital swabs. , 2012, Lab on a chip.

[23]  G. Horsman,et al.  Influence of Conserved and Hypervariable Genetic Markers on Genotyping Circulating Strains of Neisseria gonorrhoeae , 2011, PloS one.

[24]  C Bignell,et al.  UK national guideline for the management of gonorrhoea in adults, 2011 , 2011, International journal of STD & AIDS.

[25]  M. Unemo,et al.  Evaluation of Six Commercial Nucleic Acid Amplification Tests for Detection of Neisseria gonorrhoeae and Other Neisseria Species , 2011, Journal of Clinical Microbiology.

[26]  R. Sweet,et al.  Gonorrhea infection in women: prevalence, effects, screening, and management , 2011, International journal of women's health.

[27]  Linda M. Pilarski,et al.  On-chip PCR amplification of genomic and viral templates in unprocessed whole blood , 2011 .

[28]  T. Planche,et al.  Positive predictive value of the Becton Dickinson VIPER system and the ProbeTec GC Qx assay, in extracted mode, for detection of Neisseria gonorrhoeae , 2010, Sexually Transmitted Infections.

[29]  M. Unemo,et al.  Phenotypic and genetic characterization of the 2008 WHO Neisseria gonorrhoeae reference strain panel intended for global quality assurance and quality control of gonococcal antimicrobial resistance surveillance for public health purposes. , 2009, The Journal of antimicrobial chemotherapy.

[30]  A. Jolly,et al.  Comparison of Neisseria gonorrhoeae Multiantigen Sequence Typing and porB Sequence Analysis for Identification of Clusters of N. gonorrhoeae Isolates , 2008, Journal of Clinical Microbiology.

[31]  R. Peeling,et al.  Rapid tests for sexually transmitted infections (STIs): the way forward , 2006, Sexually Transmitted Infections.

[32]  M. Makoka,et al.  Evaluation of syndromic management of sexually transmitted infections in Blantyre, Malawi. , 2004, Malawi medical journal : the journal of Medical Association of Malawi.

[33]  E. Hook,et al.  The rapid test paradox: when fewer cases detected lead to more cases treated: a decision analysis of tests for Chlamydia trachomatis. , 1999, Sexually transmitted diseases.