In vivo structural analysis of articular cartilage using diffusion tensor magnetic resonance imaging.

PURPOSE The articular cartilage is a small tissue with a matrix structure of three layers between which the orientation of collagen fiber differs. A diffusion-weighted twice-refocused spin-echo echo-planar imaging (SE-EPI) sequence was optimized for the articular cartilage, and the structure of the three layers of human articular cartilage was imaged in vivo from diffusion tensor images. MATERIALS AND METHODS The subjects imaged were five specimens of swine femur head after removal of the flesh around the knee joint, five specimens of swine articular cartilage with flesh present and the knee cartilage of five adult male volunteers. Based on diffusion-weighted images in six directions, the mean diffusivity (MD) and the fractional anisotropy (FA) values were calculated. RESULTS Diffusion tensor images of the articular cartilage were obtained by sequence optimization. The MD and FA value of the specimens (each of five examples) under different conditions were estimated. Although the articular cartilage is a small tissue, the matrix structure of each layer in the articular cartilage was obtained by SE-EPI sequence with GRAPPA. The MD and FA values of swine articular cartilage are different between the synovial fluid and saline. In human articular cartilage, the load of the body weight on the knee had an effect on the FA value of the surface layer of the articular cartilage. CONCLUSION This method can be used to create images of the articular cartilage structure, not only in vitro but also in vivo. Therefore, it is suggested that this method should support the elucidation of the in vivo structure and function of the knee joint and might be applied to clinical practice.

[1]  I. Clarke,et al.  Articular cartilage: a review and scanning electron microscope study. 1. The interterritorial fibrillar architecture. , 1971, The Journal of bone and joint surgery. British volume.

[2]  P. Grenier,et al.  MR imaging of intravoxel incoherent motions: application to diffusion and perfusion in neurologic disorders. , 1986, Radiology.

[3]  R. Putz,et al.  Functional anatomy of articular cartilage under compressive loading Quantitative aspects of global, local and zonal reactions of the collagenous network with respect to the surface integrity. , 2002, Osteoarthritis and cartilage.

[4]  L W Jelinski,et al.  Self-diffusion monitors degraded cartilage. , 1995, Archives of biochemistry and biophysics.

[5]  H J Mankin,et al.  Articular cartilage: tissue design and chondrocyte-matrix interactions. , 1998, Instructional course lectures.

[6]  J. Tsuruda,et al.  Diffusion-weighted MR imaging of anisotropic water diffusion in cat central nervous system. , 1990, Radiology.

[7]  J Silvennoinen,et al.  T2 relaxation reveals spatial collagen architecture in articular cartilage: A comparative quantitative MRI and polarized light microscopic study , 2001, Magnetic resonance in medicine.

[8]  Xiang Deng,et al.  Diffusion tensor imaging of native and degenerated human articular cartilage. , 2007, Magnetic resonance imaging.

[9]  P. Basser,et al.  A simplified method to measure the diffusion tensor from seven MR images , 1998, Magnetic resonance in medicine.

[10]  V. Mlynárik,et al.  Investigation of apparent diffusion constant as an indicator of early degenerative disease in articular cartilage , 2003, Journal of magnetic resonance imaging : JMRI.

[11]  T. Chenevert,et al.  Anisotropic diffusion in human white matter: demonstration with MR techniques in vivo. , 1990, Radiology.

[12]  P. Basser,et al.  MR diffusion tensor spectroscopy and imaging. , 1994, Biophysical journal.

[13]  J. Pekar,et al.  Echo-planar imaging of intravoxel incoherent motion. , 1990, Radiology.

[14]  C F Hazlewood,et al.  Nuclear magnetic resonance measurement of skeletal muscle: anisotrophy of the diffusion coefficient of the intracellular water. , 1976, Biophysical journal.

[15]  J M Pope,et al.  Structural adaptations in compressed articular cartilage measured by diffusion tensor imaging. , 2008, Osteoarthritis and cartilage.

[16]  Robin M Heidemann,et al.  Generalized autocalibrating partially parallel acquisitions (GRAPPA) , 2002, Magnetic resonance in medicine.

[17]  V. Wedeen,et al.  Reduction of eddy‐current‐induced distortion in diffusion MRI using a twice‐refocused spin echo , 2003, Magnetic resonance in medicine.

[18]  J. E. Tanner,et al.  Spin diffusion measurements : spin echoes in the presence of a time-dependent field gradient , 1965 .

[19]  F Calamante,et al.  Effects of diffusion anisotropy on lesion delineation in a rat model of cerebral ischemia , 1997, Magnetic resonance in medicine.

[20]  P. Basser,et al.  Diffusion tensor MR imaging of the human brain. , 1996, Radiology.

[21]  J. Kärger,et al.  Self‐diffusion of water in cartilage and cartilage components as studied by pulsed field gradient NMR , 1999, Magnetic resonance in medicine.

[22]  M F Reiser,et al.  High‐resolution diffusion tensor imaging of human patellar cartilage: Feasibility and preliminary findings , 2005, Magnetic resonance in medicine.

[23]  J. Pekar,et al.  MR color mapping of myelin fiber orientation. , 1991, Journal of computer assisted tomography.

[24]  P. Basser,et al.  Estimation of the effective self-diffusion tensor from the NMR spin echo. , 1994, Journal of magnetic resonance. Series B.

[25]  H. Imhof,et al.  The role of relaxation times in monitoring proteoglycan depletion in articular cartilage , 1999, Journal of magnetic resonance imaging : JMRI.

[26]  M Hoehn-Berlage,et al.  Regional and directional anisotropy of apparent diffusion coefficient in rat brain , 1999, NMR in biomedicine.

[27]  D. Burstein,et al.  Diffusion of small solutes in cartilage as measured by nuclear magnetic resonance (NMR) spectroscopy and imaging , 1993, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[28]  M. Horsfield,et al.  Optimal strategies for measuring diffusion in anisotropic systems by magnetic resonance imaging , 1999, Magnetic resonance in medicine.

[29]  J. Mansour,et al.  Repair of large full-thickness articular cartilage defects with allograft articular chondrocytes embedded in a collagen gel. , 1998, Tissue engineering.

[30]  T J Mosher,et al.  Human articular cartilage: influence of aging and early symptomatic degeneration on the spatial variation of T2--preliminary findings at 3 T. , 2000, Radiology.

[31]  D. Le Bihan,et al.  Intravoxel incoherent motion imaging using steady-state free precession , 1988 .

[32]  H J Mankin,et al.  Articular cartilage: degeneration and osteoarthritis, repair, regeneration, and transplantation. , 1998, Instructional course lectures.

[33]  J M Pope,et al.  Diffusion tensor imaging of articular cartilage as a measure of tissue microstructure. , 2006, Osteoarthritis and cartilage.

[34]  R. Turner,et al.  Echo-planar imaging: magnetic resonance imaging in a fraction of a second. , 1991, Science.

[35]  D. Le Bihan,et al.  Diffusion tensor imaging: Concepts and applications , 2001, Journal of magnetic resonance imaging : JMRI.