Effect of Dodder Medicated Serum on the Osteoblasts Metabolism in vitro

Objective:To explore the effect of total flavones from Cuscuta chinensis(TFCC)on the rat osteoblasts metabolism in vitro.Method: Improved tissue block culture was used.Osteoblast from newly born SD rats was used.MTT,PNPP,RIA and ELISA were used separately to observe the proliferation,the activity of alkaline phosphatase(ALP),secretion of osteocalcin(BGP) and Collagen Type I(COL-I) of osteoblasts cultured in vitro with TFCC of different concentration(0.01,0.1,1,10,100 μg·L-1) in it for different incubation periods.Result: After 48 h and 72 h,5%TFCC had the effect on stimulating cell proliferation and activity of ALP;and TFCC promoted secretion of BGP and COL-I after 48 h of cultured osteoblast.Conclusion: TFCC can significantly promote the proliferation and the differentiation,and may influence the bone metabolism by promoting the secretion of COL-I.