A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count

Background and objectives: Rat experimentation is the first line of research by which a medical hypothesis is usually tested. Platelet Rich Fibrin (PRF) is a relatively new bio-material that has shown promise to enhance healing in the field of bone research and tissue engineering. In order to perform PRF based experiments on rats, a proper protocol of obtaining PRF from rats needs to be established. Materials and Methods: 35 Wistar rats were used to obtain PRF by using cardiac puncture blood draw and quick subsequent centrifugation. The PRF samples wereanalyzed and compared to standard literature PRF composition. Results: PRF samples analysis showed persistent results pertaining to known PRF composition. Conclusions: Our experiment has shown that our protocol of obtaining PRF is capable of providing high quality PRF from rats.

[1]  Anne Whittaker.  Antigens , 2020, Encyclopedia of Behavioral Medicine.

[2]  Zuoliang Qi,et al.  Letter to the editor: The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats. , 2019, Bone.

[3]  S. Raafat.  Response to: Letter to the editor: The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats. , 2019, Bone.

[4]  A. El‐Khatib,et al.  The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats. , 2018, Bone.

[5]  L. Mogoantă,et al.  Osseointegration Evaluation of Two Socket Preservation Materials in Small Diameter Bone Cavities An in vivo lab rats study , 2018, Revista de Chimie.

[6]  A. Prodromidou,et al.  Comment on ‘Role of platelet-rich fibrin on intestinal anastomosis wound healing in a rat’ , 2018, Biomedical materials.

[7]  N. Özçay.  Reply to Comment on ‘Role of platelet-rich fibrin on intestinal anastomosis wound healing in a rat’ , 2018, Biomedical materials.

[8]  M. Onger,et al.  Comparison of the Regenerative Effects of Platelet-Rich Fibrin and Plasma Rich in Growth Factors on Injured Peripheral Nerve: An Experimental Study. , 2018, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[9]  N. Özçay,et al.  Role of platelet-rich fibrin on intestinal anastomosis wound healing in a rat , 2018, Biomedical materials.

[10]  I. Sarici,et al.  Comparison of platelet rich plasma versus fibrin glue on colonic anastomoses in rats. , 2018, Acta cirurgica brasileira.

[11]  Nan Li,et al.  How novice, skilled and advanced clinical researchers include variables in a case report form for clinical research: a qualitative study , 2017, BMJ Open.

[12]  T. Dülgeroğlu,et al.  Evaluation of the Effect of Platelet-Rich Fibrin on Long Bone Healing: An Experimental Rat Model. , 2017, Orthopedics.

[13]  D. Süren,et al.  The effect of platelet-rich fibrin membrane on the repair of perforated tympanic membrane: an experimental study , 2017, Acta oto-laryngologica.

[14]  Taiji Suzuki,et al.  An Evaluation of the Accuracy of the Subtraction Method Used for Determining Platelet Counts in Advanced Platelet-Rich Fibrin and Concentrated Growth Factor Preparations , 2017, Dentistry journal.

[15]  L. Mogoantă,et al.  An evaluation of a collagen-based material osseointegration. , 2017, Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie.

[16]  S. Hostiuc,et al.  Three-dimensional organ scanning reveals brain edema reduction in a rat model of stroke treated with an aquaporin 4 inhibitor. , 2017, Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie.

[17]  S. Kuroda,et al.  Stability of Platelet-rich Fibrin in Vivo: Histological Study in Rats , 2016 .

[18]  L. Mogoantă,et al.  Antifibrotic action of telmisartan in experimental carbon tetrachloride-induced liver fibrosis in Wistar rats. , 2016, Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie.

[19]  J. Braga–Silva,et al.  Platelet-Rich Fibrin Promotes an Accelerated Healing of Achilles Tendon When Compared to Platelet-Rich Plasma in Rat , 2015, World journal of plastic surgery.

[20]  K. Goosens,et al.  Sampling Blood from the Lateral Tail Vein of the Rat , 2015, Journal of visualized experiments : JoVE.

[21]  T. Inomata,et al.  Vital signs monitoring during injectable and inhalant anesthesia in mice , 2014, Experimental animals.

[22]  S. Burchielli,et al.  An Autologously Generated Platelet-Rich Plasma Suturable Membrane May Enhance Peripheral Nerve Regeneration after Neurorraphy in an Acute Injury Model of Sciatic Nerve Neurotmesis , 2014, Journal of Reconstructive Microsurgery.

[23]  D. M. Dohan Ehrenfest,et al.  Classification of platelet concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectives. , 2014, Muscles, ligaments and tendons journal.

[24]  D. Gheonea,et al.  Optical coherence tomography investigation of ischemic stroke inside a rodent model. , 2014, Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie.

[25]  J. Dixit,et al.  Periodontal regeneration in deep intrabony periodontal defect using hydroxyapatite particles with platelet rich fibrin membrane-a case report. , 2011, Journal of oral biology and craniofacial research.

[26]  M. Zumstein,et al.  The Future of Platelet Concentrates in Sports Medicine: Platelet-Rich Plasma, Platelet-Rich Fibrin, and the Impact of Scaffolds and Cells on the Long-term Delivery of Growth Factors , 2011 .

[27]  D. M. Ehrenfest,et al.  How to optimize the preparation of leukocyte- and platelet-rich fibrin (L-PRF, Choukroun's technique) clots and membranes: introducing the PRF Box. , 2010 .

[28]  S. Parasuraman,et al.  Blood sample collection in small laboratory animals , 2010, Journal of pharmacology & pharmacotherapeutics.

[29]  D. M. Dohan Ehrenfest,et al.  Three-dimensional architecture and cell composition of a Choukroun's platelet-rich fibrin clot and membrane. , 2010, Journal of periodontology.

[30]  D. M. Dohan Ehrenfest.  How to optimize the preparation of leukocyte- and platelet-rich fibrin (L-PRF, Choukroun's technique) clots and membranes: introducing the PRF Box. , 2010, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[31]  L. Jørgensen,et al.  Effect of Topical Autologous Platelet-Rich Fibrin versus No Intervention on Epithelialization of Donor Sites and Meshed Split-Thickness Skin Autografts: A Randomized Clinical Trial , 2008, Plastic and reconstructive surgery.

[32]  P. O'loughlin,et al.  Selection and development of preclinical models in fracture-healing research. , 2008, The Journal of bone and joint surgery. American volume.

[33]  C. Kessler,et al.  Annexin A3 expression after stroke in the aged rat brain. , 2008, Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie.

[34]  C. Kessler,et al.  Environmental enrichment improves functional and neuropathological indices following stroke in young and aged rats. , 2007, Restorative neurology and neuroscience.

[35]  A. Dohan,et al.  Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[36]  V. Jekl,et al.  Blood sampling from the cranial vena cava in the Norway rat (Rattus norvegicus) , 2005, Laboratory animals.

[37]  Robin Hull,et al.  A good practice guide to the administration of substances and removal of blood, including routes and volumes , 2001, Journal of applied toxicology : JAT.

[38]  M D Blaufox,et al.  Blood volume in the rat. , 1985, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[39]  N. Kamada,et al.  Fully allogeneic liver grafting in rats induces a state of systemic nonreactivity to donor transplantation antigens. , 1980, Transplantation.