Phytoremediation of arsenic-contaminated water: the role of antioxidant metabolism of Azolla caroliniana Willd. (Salviniales)

ABSTRACT Phytoremediation has proven to be an effi cient technology for removing arsenic (As) from water, but the plants used in this process need to be tolerant to the damage caused by As. Th e toxic eff ect of As on growth and functioning of the antioxidant system was studied in individual plants of Azolla caroliniana exposed to fi ve concentrations of As (0.0, 0.25, 0.5, 1.0 and 1.5 mg L -1 ) for the course of fi ve days. Growth, As absorption, enzymatic activity, total and non-protein thiols and anthocyanin content were assessed. Azolla caroliniana was able to take up large amounts of the pollutant, reaching As concentrations of 386.1 µg g -1 dry weight without saturating the absorption mechanism. Th e tolerance index and the growth of A. caroliniana decreased with the increased As uptake. Superoxide dismutase, peroxidases, catalases and glutathione reductase activities increased at lower doses of As and subsequently declined with higher concentrations, whereas ascorbate peroxidase activity was reduced in all treatments. Unlike the enzymatic defence system, anthocyanin and thiol content increased consistently in all treatments and showed a positive correlation with As concentration. Th erefore, the increased synthesis of non-enzymatic antioxidants is most likely the main factor responsible for the high As tolerance of

[1]  U. Saha,et al.  Arsenic hyperaccumulating fern: Implications for remediation of arsenic contaminated soils , 2016 .

[2]  K. Siddique,et al.  Arsenic toxicity in plants: Cellular and molecular mechanisms of its transport and metabolism , 2016 .

[3]  S. Hill,et al.  A study of arsenic speciation in soil, irrigation water and plant tissue: A case study of the broad bean plant, Vicia faba. , 2016, Food chemistry.

[4]  F. Espinoza-Quiñones,et al.  Assessment of Anthropogenic Impacts on the Water Quality of Marreco River, Brazil, Based on Principal Component Analysis and Toxicological Assays , 2016, Water, Air, & Soil Pollution.

[5]  Xianjun Meng,et al.  Effects of high hydrostatic pressure on physicochemical properties, enzymes activity, and antioxidant capacities of anthocyanins extracts of wild Lonicera caerulea berry , 2016 .

[6]  C. Majumder,et al.  Sequestering of As(III) and As(V) from wastewater using a novel neem leaves/MnFe2O4 composite biosorbent , 2016, International journal of phytoremediation.

[7]  Zhao Jiang,et al.  Oxidative stress response induced in an atrazine phytoremediating plant: Physiological responses of Pennisetum glaucum to high atrazine concentrations , 2016, International journal of phytoremediation.

[8]  P. Suprasanna,et al.  Arsenic toxicity in rice (Oryza sativa L.) is influenced by sulfur supply: Impact on the expression of transporters and thiol metabolism , 2016 .

[9]  A. Nadgórska-Socha,et al.  Accumulation of heavy metals and antioxidant responses in Pinus sylvestris L. needles in polluted and non-polluted sites , 2016, Ecotoxicology.

[10]  Hui-lian Xu,et al.  Interspecific differences in growth response and tolerance to the antibiotic sulfadiazine in ten clonal wetland plants in South China. , 2016, The Science of the total environment.

[11]  O. Dhankher,et al.  Nitric Oxide Alleviated Arsenic Toxicity by Modulation of Antioxidants and Thiol Metabolism in Rice (Oryza sativa L.) , 2016, Front. Plant Sci..

[12]  S. K. Karn.  Arsenic (As) contamination: A major risk factor in Xinjiang Uyghur autonomous region of China. , 2015, Environmental pollution.

[13]  O. Dhankher,et al.  Sulfur mediated reduction of arsenic toxicity involves efficient thiol metabolism and the antioxidant defense system in rice. , 2015, Journal of hazardous materials.

[14]  N. M. Silveira,et al.  Nitric Oxide Attenuates Oxidative Stress Induced by Arsenic in Lettuce (Lactuca sativa) Leaves , 2015, Water, Air, & Soil Pollution.

[15]  M. T. Khan,et al.  Biochemical mechanisms of signaling: perspectives in plants under arsenic stress. , 2015, Ecotoxicology and environmental safety.

[16]  F. S. Farnese,et al.  Evaluation of the potential of Pistia stratiotes L. (water lettuce) for bioindication and phytoremediation of aquatic environments contaminated with arsenic. , 2014, Brazilian journal of biology = Revista brasleira de biologia.

[17]  F. S. Farnese,et al.  Sulfur metabolism: different tolerances of two aquatic macrophytes exposed to arsenic. , 2014, Ecotoxicology and environmental safety.

[18]  F. S. Farnese,et al.  Anthocyanins, thiols, and antioxidant scavenging enzymes are involved in Lemna gibba tolerance to arsenic , 2014 .

[19]  J. Oliveira,et al.  Mineral nutrition and enzymatic adaptation induced by arsenate and arsenite exposure in lettuce plants. , 2013, Plant physiology and biochemistry : PPB.

[20]  L. Zagorchev,et al.  A Central Role for Thiols in Plant Tolerance to Abiotic Stress , 2013, International journal of molecular sciences.

[21]  T. A. Adjadeh,et al.  Effect of phosphorus on the concentrations of arsenic, iron and some other elements in barley grown hydroponically , 2013 .

[22]  M. Margis-Pinheiro,et al.  Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection , 2012, Genetics and molecular biology.

[23]  Weihua Chen,et al.  Arsenic Toxicity: The Effects on Plant Metabolism , 2012, Front. Physio..

[24]  Mohammad Pessarakli,et al.  Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions , 2012 .

[25]  A. Alarcón,et al.  Short-Term Effects of Arsenate-Induced Toxicity on Growth, Chlorophyll and Carotenoid Contents, and Total Content of Phenolic Compounds of Azolla filiculoides , 2011 .

[26]  G. Agati,et al.  Multiple functional roles of flavonoids in photoprotection. , 2010, The New phytologist.

[27]  P. Trivedi,et al.  Effect of arsenic on growth, oxidative stress, and antioxidant system in rice seedlings. , 2009, Ecotoxicology and environmental safety.

[28]  J. Férard,et al.  Induction of oxidative stress biomarkers associated with heavy metal stress in Fontinalis antipyretica Hedw. , 2009, Chemosphere.

[29]  M. Iqbal,et al.  Modulation of antioxidant defence system for arsenic detoxification in Indian mustard. , 2009, Ecotoxicology and environmental safety.

[30]  Yong-guan Zhu,et al.  Arsenic accumulation by the aquatic fern Azolla: comparison of arsenate uptake, speciation and efflux by A. caroliniana and A. filiculoides. , 2008, Environmental pollution.

[31]  N. Inoue,et al.  Assessment of Potential Indigenous Plant Species for the Phytoremediation of Arsenic-Contaminated Areas of Bangladesh , 2008, International journal of phytoremediation.

[32]  J. Franco-Zorrilla,et al.  A Mutant of the Arabidopsis Phosphate Transporter PHT1;1 Displays Enhanced Arsenic Accumulation , 2007, The Plant Cell Online.

[33]  M. Vasilakakis,et al.  Effect of propagation material on some quality attributes of strawberry fruit (Fragaria x ananassa, var. Selva) , 2006 .

[34]  Z. Stępniewska,et al.  The ability of Azolla caroliniana to remove heavy metals (Hg(II), Cr(III), Cr(VI)) from municipal waste water. , 2004, Chemosphere.

[35]  K.,et al.  Changes in Isozyme Profiles of Catalase, Peroxidase, and Glutathione Reductase during Acclimation to Chilling in Mesocotyls of Maize Seedlings , 1995, Plant physiology.

[36]  S. Pezeshki,et al.  Effect of dimethylarsenic acid (DMAA) on growth, tissue arsenic, and photosynthesis of rice plants , 1993 .

[37]  P. Meuwly,et al.  Alteration of thiol pools in roots and shoots of maize seedlings exposed to cadmium : adaptation and developmental cost. , 1992, Plant physiology.

[38]  E. Havir,et al.  Biochemical and developmental characterization of multiple forms of catalase in tobacco leaves. , 1987, Plant physiology.

[39]  K. Asada,et al.  Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts , 1981 .

[40]  R. Hunt.  Plant growth analysis , 1980 .

[41]  D. Wilkins.  THE MEASUREMENT OF TOLERANCE TO EDAPHIC FACTORS BY MEANS OF ROOT GROWTH , 1978 .

[42]  C. N. Giannopolitis,et al.  Superoxide dismutases: I. Occurrence in higher plants. , 1977, Plant physiology.

[43]  R. Clárk.  Characterization of phosphatase of intact maize roots. , 1975, Journal of agricultural and food chemistry.

[44]  I. Fridovich,et al.  Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. , 1971, Analytical biochemistry.

[45]  Nannan Li,et al.  Arsenic Uptake and Translocation in Plants. , 2016, Plant & cell physiology.

[46]  C. Ribeiro,et al.  Arsenic toxicity: cell signalling and the attenuating effect of nitric oxide in Eichhornia crassipes , 2015, Biologia Plantarum.

[47]  N. Cedergreen,et al.  Biomarkers in aquatic plants: selection and utility. , 2009, Reviews of environmental contamination and toxicology.

[48]  John Shi,et al.  Antioxidant properties of anthocyanins extracted from litchi (Litchi chinenesis Sonn.) fruit pericarp tissues in relation to their role in the pericarp browning , 2007 .

[49]  P. R. Mosquim,et al.  Aluminum effects on lipid peroxidation and on the activities of enzymes of oxidative metabolism in sorghum. , 1999 .

[50]  B. Chance,et al.  ASSAY OF CATALASES AND PEROXIDASES, IN METHODS IN ENZYMOLOGY , 1995 .

[51]  I. Carlberg,et al.  Glutathione reductase. , 1985, Methods in enzymology.

[52]  J. Sedlák,et al.  Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. , 1968, Analytical biochemistry.