PRODUCTION OF TANNASE UNDER SOLID-STATE FERMENTATION AND ITS APPLICATION IN DETANNIFICATION OF GUAVA JUICE

Guava juice is known to be rich in antioxidant activity due a high level of vitamins A and C. However, tannins present in the guava juice form tannin–protein complexes that affect the utilization of vitamins and minerals and inhibit digestive enzymes. Beside this, bitterness and cloudiness are the other major problems of juice industries. The present study aimed to utilize a low-cost substrate (tea residue) for the production of tannase and its application in detannification of guava juice. Solid-state fermentation (SSF) was evaluated to produce tannase from Aspergillus niger. Maximum tannase (1.86 U/g dry substrate) production was observed at 30°C after 96 hr of the incubation period. The optimum pH of the moistening agent was found to be 5.0. Partially purified enzyme using ammonium sulfate precipitation was subjected to guava juice treatment at a level of 0.5, 1.0, and 2.0% for 30 and 60 min. Decrease in tannic acid content of guava juice was found to be 17.60, 29.04, and 44.38% after 30 min and 40.59, 53.69, and 59.23% after 60 min, respectively.

[1]  Ashok Kumar,et al.  Production of tannase through solid state fermentation using Indian Rosewood (Dalbergia Sissoo)sawdust—a timber industry waste , 2012, Annals of Microbiology.

[2]  Cristóbal N. Aguilar,et al.  Optimization of tannase production by Aspergillus niger in solid-state packed-bed bioreactor. , 2011, Journal of microbiology and biotechnology.

[3]  S. Gautam,et al.  Tannase Production by Penicillium Atramentosum KM under SSF and its Applications in Wine Clarification and Tea Cream Solubilization , 2011, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].

[4]  Anil Kumar,et al.  Purification and characterization of extracellular tannin acyl hydrolase from Aspergillus heteromorphus MTCC 8818 , 2010 .

[5]  S. Murugesh,et al.  Manipulation of fermentation conditions on production of Tannase from agricultural by-products with Aspergillus oryzae , 2010 .

[6]  L. Gonçalves,et al.  Effects of inoculum concentration, temperature, and carbon sources on tannase production during solid state fermentation of cashew apple bagasse , 2008 .

[7]  Manjit,et al.  Tannase production by Aspergillus fumigatus MA under solid-state fermentation , 2008 .

[8]  V. Vivekanand,et al.  Solid-state fermentation for gluconic acid production from sugarcane molasses by Aspergillus niger ARNU-4 employing tea waste as the novel solid support. , 2008, Bioresource Technology.

[9]  Rakesh Kumar,et al.  Production of tannase from Aspergillus ruber under solid-state fermentation using jamun (Syzygium cumini) leaves. , 2007, Microbiological research.

[10]  C. Lindner,et al.  The effect of sulphur dioxide in sugar on the corrosion of plain cans with grapefruit segments in syrup , 2007 .

[11]  Cristóbal N. Aguilar,et al.  Gallic acid and tannase accumulation during fungal solid state culture of a tannin-rich desert plant (Larrea tridentata Cov.). , 2007, Bioresource technology.

[12]  R. Banerjee,et al.  Production of tannase under mSSF and its application in fruit juice debittering , 2006 .

[13]  Ashok Pandey,et al.  Purification and Characterization of Tannin Acyl Hydrolase from Aspergillus niger ATCC 16620 , 2005 .

[14]  B. Singh,et al.  Microbial degradation of tannins – A current perspective , 2004, Biodegradation.

[15]  Cristóbal N. Aguilar,et al.  Review: Sources, Properties, Applications and Potential uses of Tannin Acyl Hydrolase , 2001 .

[16]  B. Pati,et al.  Colorimetric assay method for determination of the tannin acyl hydrolase (EC 3.1.1.20) activity. , 2001, Analytical biochemistry.

[17]  M. Raimbault,et al.  General and microbiological aspects of solid substrate fermentation , 1998 .

[18]  Ashok Pandey,et al.  Biosynthesis of glucoamylase from Aspergillus niger by solid-state fermentation using tea waste as the basis of a solid substrate , 1998 .

[19]  B. K. Lonsane,et al.  Production and application of tannin acyl hydrolase: state of the art. , 1997, Advances in applied microbiology.

[20]  B. C. Bhattacharyya,et al.  Optimization of tannase biosynthesis by a newly isolated Rhizopus oryzae , 1994 .

[21]  J. Lebeault,et al.  Production of Tannase and Degradation of Chestnut Tannin by Bacteria , 1983 .

[22]  L. Butler,et al.  Protein precipitation method for the quantitative determination of tannins , 1978 .