Relationship of hepatitis B virus infection of placental barrier and hepatitis B virus intra-uterine transmission mechanism.

AIM To explore the mechanism of intra-uterine transmission, the HBV infection status of placental tissue and in vitro cultured placental trophoblastic cells was tested through in vivo and in vitro experiments. METHODS A variety of methods, such as ELISA, RT-PCR, IHC staining and immunofluorescent staining were employed to test the HBV marker positive pregnant women's placenta and in vitro cultured placental trophoblastic cells. RESULTS The HBV DNA levels in pregnant women's serum and fetal cord blood were correlated. For those cord blood samples positive for HBV DNA, their maternal blood levels of HBV DNA were at a high level. The HBsAg IHC staining positive cells could be seen in the placental tissues and the presence of HBV DNA detected. After co-incubating the trophoblastic cells and HBV DNA positive serum in vitro, the expressions of both HBsAg and HBV DNA could be detected. CONCLUSION The mechanism of HBV intra-uterine infection may be due to that HBV breaches the placental barrier and infects the fetus.

[1]  David A. Anderson,et al.  Hepatitis B Virus Translocates across a Trophoblastic Barrier , 2007, Journal of Virology.

[2]  Xiao-Min Xiao,et al.  Prevention of vertical hepatitis B transmission by hepatitis B immunoglobulin in the third trimester of pregnancy , 2007, International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics.

[3]  Zhi-ying Zhang,et al.  Maternal hepatitis B virus (HBV) DNA positivity and sexual intercourse are associated with HBV intrauterine transmission in China: A prospective case–control study , 2007, Journal of gastroenterology and hepatology.

[4]  C. Kripke.  Hepatitis B vaccine for infants of HBsAg-positive mothers. , 2007, American family physician.

[5]  Yu-zhu Yin,et al.  [Intrauterine HBV infection: risk factors and impact of HBV DNA]. , 2006, Nan fang yi ke da xue xue bao = Journal of Southern Medical University.

[6]  H. Whittle,et al.  Long-term protection against carriage of hepatitis B virus after infant vaccination. , 2006, The Journal of infectious diseases.

[7]  O. Genbačev,et al.  Maternal antibodies enhance or prevent cytomegalovirus infection in the placenta by neonatal Fc receptor-mediated transcytosis. , 2006, The American journal of pathology.

[8]  E. Maidji,et al.  Patterns of human cytomegalovirus infection in term placentas: a preliminary analysis. , 2006, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology.

[9]  Jian-She Wang,et al.  Transformation of hepatitis B serologic markers in babies born to hepatitis B surface antigen positive mothers. , 2005, World journal of gastroenterology.

[10]  De-zhong Xu,et al.  [Study on the presence of hepatitis B virus in first-trimester villi in pregnant women with hepatitis B surface antigen positive]. , 2005, Zhonghua fu chan ke za zhi.

[11]  De-zhong Xu,et al.  Uptake of hepatitis B virus into choriocarcinoma cells in the presence of proinflammatory cytokine tumor necrosis factor-alpha. , 2004, American journal of obstetrics and gynecology.

[12]  Hong Yang,et al.  Quantitative analysis of HBV DNA level and HBeAg titer in hepatitis B surface antigen positive mothers and their babies: HBeAg passage through the placenta and the rate of decay in babies , 2003, Journal of medical virology.

[13]  M. Tremblay,et al.  The Low Viral Production in Trophoblastic Cells Is Due to a High Endocytic Internalization of the Human Immunodeficiency Virus Type 1 and Can Be Overcome by the Pro-inflammatory Cytokines Tumor Necrosis Factor-α and Interleukin-1* , 2003, The Journal of Biological Chemistry.

[14]  M. Muñoz-Fernandez,et al.  Transmission of HIV-1 infection between trophoblast placental cells and T-cells take place via an LFA-1-mediated cell to cell contact. , 2003, Virology.

[15]  J. Jordan,et al.  Apoptotic activity in villous trophoblast cells during B19 infection correlates with clinical outcome: assessment by the caspase-related M30 Cytodeath antibody. , 2002, Placenta.

[16]  Ying-lin Liu,et al.  [Analysis about clinical data of intrauterine infection of hepatitis B virus]. , 2002, Zhonghua fu chan ke za zhi.

[17]  R. Doms,et al.  Placental expression of DC‐SIGN may mediate intrauterine vertical transmission of HIV , 2001, The Journal of pathology.

[18]  Pengbo Liu,et al.  Role of placental tissues in the intrauterine transmission of hepatitis B virus. , 2001, American journal of obstetrics and gynecology.

[19]  J. G. Estrada,et al.  Placental cytokine and chemokine production in HIV‐1‐infected women: trophoblast cells show a different pattern compared to cells from HIV‐negative women , 2001, Clinical and experimental immunology.

[20]  F. Barré-Sinoussi,et al.  Cell-to-Cell Contact Results in a Selective Translocation of Maternal Human Immunodeficiency Virus Type 1 Quasispecies across a Trophoblastic Barrier by both Transcytosis and Infection , 2001, Journal of Virology.

[21]  C. Casper,et al.  Mother-to-child transmission of transmission of HIV-1: The role of HIV-1 variability and the placental barrier , 2001 .

[22]  E. Fenyö,et al.  Mother-to-child transmission of HIV-1: the role of HIV-1 variability and the placental barrier. , 2001, Acta microbiologica et immunologica Hungarica.

[23]  Y. Nagata,et al.  Apoptosis in placentas from human T-lymphotropic virus type I-seropositive pregnant women: a possible defense mechanism against transmission from mother to fetus. , 1999, Obstetrics and gynecology.

[24]  X. Yang,et al.  Prevention of intrauterine infection by hepatitis B virus with hepatitis B immune globulin: efficacy and mechanism. , 1999, Chinese medical journal.

[25]  G. Burton,et al.  The Structure of the Human Placenta: Implications for Initiating and Defending Against Virus Infections , 1997, Reviews in medical virology.

[26]  J. Best.  Laboratory diagnosis of intrauterine and perinatal virus infections. , 1996, Clinical and diagnostic virology.

[27]  Keith Dudley.  Short protocols in molecular biology , 1990 .

[28]  F. di Nola,et al.  [Viral hepatitis and pregnancy]. , 1967, Minerva medica.