Genetic Mode of Bt Insecticidal Protein Gene in the Insect-Resistant Transgenic Rapeseed

To select and breed stable transgenic insecticidal rapeseed,by detecting the progeny of transgenic rapeseed plants with Bt insecticidal protein gene via kanamycin sifting and PCR,genetic mode of Bt insecticidal protein gene in the insect resistant transgenic rapeseed was studied.The results show that single copy of Bt insecticidal protein gene has been integrated into the genome of T 0 transgenic plants and stably inherited. By analysing F 2 of hybrid combination of homozygous transgenic plants, Bt insecticidal protein gene has been integrated into different locus of homologous chromosome of T 01 and T 05 or T 03 and T 08 transgenic rapeseed plants,but has been integrated into heterologous chromosome.