Changes in receptor-mediated endocytosis in liver sinusoidal cells after partial hepatectomy in the rat.

Liver sinusoidal cells play an important role in host defense by clearing particulate matter and macromolecules from the circulation. In this study, receptor-mediated endocytosis in sinusoidal cells was examined in two-thirds hepatectomized rats using 125I-labeled formaldehyde-treated bovine serum albumin (fBSA) as an endocytable macromolecule. The liver-weight to body-weight ratio in hepatectomized rats returned to the control value 10 days after hepatectomy. The endocytotic index for fBSA in sinusoidal cells decreased significantly to 0.0210 +/- 0.0017 (controls, 0.0598 +/- 0.0019) on the first day, then returned to the control level at 5 days (0.0554 +/- 0.0030). The changes in hepatic uptake for fBSA showed a similar time course of the endocytotic index. A transient increase in the uptake of fBSA per unit weight of liver of 22-39% above control occurred 2 to 3 days after hepatectomy. In contrast to fBSA, the endocytotic index in hepatocytes evaluated with 125I-labeled asialofetuin reached the minimum level on the second day, and then recovered to the control level 10 days after hepatectomy. These results suggest that endocytosis of fBSA by sinusoidal cells decreases after hepatectomy and rapidly recovers to normal before the completion of liver regeneration, whereas endocytosis of asialofetuin by hepatocytes decreases following hepatic resection and returns to normal when regeneration is substantially complete.

[1]  M. W. Flye,et al.  Prostaglandin E2 production during hepatic regeneration downregulates Kupffer cell IL-6 production. , 1992, Annals of surgery.

[2]  M. W. Flye,et al.  Kupffer cell autoregulation of IL-1 production by PGE2 during hepatic regeneration. , 1992, The Journal of surgical research.

[3]  G. Molema,et al.  Formaldehyde treated albumin contains monomeric and polymeric forms that are differently cleared by endothelial and Kupffer cells of the liver: evidence for scavenger receptor heterogeneity. , 1991, Biochemical and biophysical research communications.

[4]  T. Gjøen,et al.  Lysosomal and endosomal heterogeneity in the liver: A comparison of the intracellular pathways of endocytosis in rat liver cells , 1991, Hepatology.

[5]  K. Fujiwara,et al.  Provocation of massive hepatic necrosis by endotoxin after partial hepatectomy in rats. , 1990, Gastroenterology.

[6]  K. Wake,et al.  LARGE GRANULAR LYMPHOCYTES AND KUPFFER CELLS IN REGENERATING RAT LIVER , 1990 .

[7]  B. Smedsrød,et al.  Scavenger functions of the liver endothelial cell. , 1990, The Biochemical journal.

[8]  G. Michalopoulos.  Liver regeneration: molecular mechanisms of growth control , 1990, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[9]  F. Katsumoto,et al.  Stimulation of DNA synthesis in hepatocytes by kupffer cells after partial hepatectomy , 1989, Hepatology.

[10]  B. Smedsrød,et al.  Intracellular transport of formaldehyde-treated serum albumin in liver endothelial cells after uptake via scavenger receptors. , 1989, The Biochemical journal.

[11]  G. Andersson,et al.  Asialoglycoprotein receptors in rat liver nodules. , 1988, Carcinogenesis.

[12]  S. Arii,et al.  The three different phases of reticuloendothelial system phagocytic function in rats with liver injury. , 1988, The Journal of surgical research.

[13]  T. V. van Berkel,et al.  The interaction in vivo of transferrin and asialotransferrin with liver cells. , 1987, The Biochemical journal.

[14]  D. Cikrit,et al.  Bacterial clearance in the intact and regenerating liver. , 1985, Journal of pediatric surgery.

[15]  S. Tanase,et al.  Plasma clearance of intravenously injected aspartate aminotransferase isozymes: Evidence for preferential uptake by sinusoidal liver cells , 1985, Hepatology.

[16]  H. Maeda,et al.  Scavenger function of sinusoidal liver cells. Acetylated low-density lipoprotein is endocytosed via a route distinct from formaldehyde-treated serum albumin. , 1985, The Journal of biological chemistry.

[17]  E. Wisse,et al.  Importance of Local Proliferation in the Expanding Kupffer Cell Population of Rat Liver After Zymosan Stimulation and Partial Hepatectomy , 1984, Hepatology.

[18]  R. Blomhoff,et al.  Endocytosis of formaldehyde-treated serum albumin via scavenger pathway in liver endothelial cells. , 1984, The Biochemical journal.

[19]  H. Dalton,et al.  Purification and characterization of component A of the methane monooxygenase from Methylococcus capsulatus (Bath). , 1984, The Journal of biological chemistry.

[20]  H. Kolb,et al.  GalNAc/Gal‐specific rat liver lectins: their role in cellular recognition , 1984, Biology of the cell.

[21]  H. Kolb,et al.  Electron microscopic evidence for an asialoglycoprotein receptor on kupffer cells: Localization of lectin-mediated endocytosis , 1982, Cell.

[22]  D. J. Howard,et al.  Asialoglycoprotein receptors in hepatic regeneration. , 1982, The Journal of biological chemistry.

[23]  J S Fleming,et al.  In vivo measurement of liver perfusion in the normal and partially hepatectomized rat using Tc-99m sulfur colloid. , 1979, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[24]  D. Knook,et al.  Isolation and characterization of Kupffer and endothelial cells from the rat liver. , 1977, Experimental cell research.

[25]  K. Norum,et al.  Uptake and degradation of 125I-labelled asialo-fetuin by isolated rat hepatocytes. , 1977, Biochimica et biophysica acta.

[26]  R. Cotran,et al.  MONONUCLEAR PHAGOCYTES (KUPFFER CELLS) AND ENDOTHELIAL CELLS , 1972, The Journal of cell biology.

[27]  G. Gregoriadis,et al.  The role of sialic acid in determining the survival of glycoproteins in the circulation. , 1971, The Journal of biological chemistry.

[28]  J. Marchalonis.  An enzymic method for the trace iodination of immunoglobulins and other proteins. , 1969, The Biochemical journal.

[29]  J. Fabrikant.  THE KINETICS OF CELLULAR PROLIFERATION IN REGENERATING LIVER , 1968, The Journal of cell biology.

[30]  J. L. Mego,et al.  THE USE OF FORMALDEHYDE-TREATED 131I-ALBUMIN IN THE STUDY OF DIGESTIVE VACUOLES AND SOME PROPERTIES OF THESE PARTICLES FROM MOUSE LIVER , 1967, The Journal of cell biology.

[31]  J. L. Edwards,et al.  PARENCHYMAL AND LITTORAL CELL PROLIFERATION DURING LIVER REGENERATION. , 1964, Laboratory investigation; a journal of technical methods and pathology.

[32]  J. Grisham,et al.  A morphologic study of deoxyribonucleic acid synthesis and cell proliferation in regenerating rat liver; autoradiography with thymidine-H3. , 1962, Cancer research.

[33]  R. Brauer,et al.  Liver function in regenerating rat liver. CrPO4 colloid uptake and bile flow. , 1959, The American journal of physiology.

[34]  G. Higgins,et al.  Experimental pathology of the liver , 1931 .