Influence of dietary phosphorus deficiency with or without addition of fumaric acid to a diet in pigs on bone parameters.

The purpose of this study was to examine if substantial bone loss occurs in weaned pigs by feeding a phosphorus-deficient diet with or without fumaric acid. Eighteen weaned pigs were used. The animals were assigned to three groups: group C (control; 0.65% P on DM basis), group LP (low phosphorus; 0.37% P on DM basis) and group LPF (low phosphorus plus fumaric acid; 0.35% P on DM basis plus 2% fumaric acid). These three diets were fed to the groups for a period of four weeks after a two-week adaptation period. Blood samples were collected once a week. Carboxyterminal telopeptide of type I collagen (ICTP) in serum was used as a bone resorption marker. Osteocalcin (OC) and bone-specific alkaline phosphatase (bAP) were used as bone formation markers. Bone mineral density (BMD) and content (BMC) were determined by peripheral quantitative computer tomography. BAP activities significantly increased (24%) in group LPF, and at the last sampling day group LPF had significantly increased activities in comparison to group C. In contrast, ICTP concentrations significantly increased with time in group LP and LPF, and at the last sampling day group LPF had significantly increased activities in comparison to group C. BMD and BMC in femur and tibia significantly decreased in group LP and LPF. The results show that P-deficient diets induce a bone loss. Fumaric acid did not influence the degree of bone loss. With a better understanding of its effect on bone, dietary phosphorus requirements in pigs could be more precisely defined.

[1]  H. Coste,et al.  Pathophysiological effects of low dietary phosphorus in pigs. , 2001, Veterinary journal.

[2]  H. Poulsen.  Phosphorus Utilization and Excretion in Pig Production , 2000 .

[3]  F. Eckstein,et al.  Precision and intersite correlation of bone densitometry at the radius, tibia and femur with peripheral quantitative CT , 1999, Skeletal Radiology.

[4]  J. Risteli,et al.  Diurnal variation in concentrations of various markers of bone metabolism in dogs. , 1999, American journal of veterinary research.

[5]  J. Risteli,et al.  INFLUENCE OF LOW CALCIUM, HIGH CALCIUM AND ENERGY-RICH DIETS ON BONE MARKERS IN OVARIECTOMIZED BEAGLE DOGS , 1999 .

[6]  D. Hartmann,et al.  Biochemical markers of bone metabolism in draught andwarmblood horses , 1998 .

[7]  J. Risteli,et al.  Comparison of bone resorption markers during hypocalcemia in dairy cows. , 1998, Journal of dairy science.

[8]  W. Buchan,et al.  Effects of variations in dietary calcium and phosphorus supply on plasma and bone osteocalcin concentrations and bone mineralization in growing pigs , 1998, Experimental physiology.

[9]  G. Breur,et al.  Serum markers of bone metabolism in dogs. , 1998, American journal of veterinary research.

[10]  E. Kornegay,et al.  Phytase supplementation of low-phosphorus growing-finishing pig diets improves performance, phosphorus digestibility, and bone mineralization and reduces phosphorus excretion. , 1997, Journal of animal science.

[11]  J. Milne,et al.  Effect of diets varying in nitrogen or phosphorus content on indicators of bone growth in lambs , 1997, Experimental physiology.

[12]  G. Cromwell,et al.  The determination of serum concentrations of osteocalcin in growing pigs and its relationship to end-measures of bone mineralization. , 1996, Journal of animal science.

[13]  M. Rodehutscord,et al.  Mechanisms of intestinal phosphorus absorption and availability of dietary phosphorus in pigs. , 1996, DTW. Deutsche tierarztliche Wochenschrift.

[14]  U. Schulte,et al.  Method for determination of bone alkaline phosphatase activity: analytical performance and clinical usefulness in patients with metabolic and malignant bone diseases. , 1996, Clinical chemistry.

[15]  H. Bekaert,et al.  An estimation of the minimal P requirements for growing-finishing pigs, as influenced by the Ca level of the diet , 1995 .

[16]  M. Rodehutscord,et al.  Effects of supplemental microbial phytase on availability of phosphorus contained in maize, wheat and triticale to pigs , 1994 .

[17]  S. Robins,et al.  Effects of low phosphate intake on bone and mineral metabolism and microbial protein synthesis in lambs , 1994, Experimental physiology.

[18]  L. Deftos,et al.  Associations and dissociations between serum bone gla protein and alkaline phosphatase in skeletal metabolism , 1993, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[19]  A. Hennig,et al.  Untersuchungen zum einfluss der phosphor‐versorgung auf den energieumsatz wachsender schweine , 1993 .

[20]  R. van der Meer,et al.  Interaction of calcium and phosphate decreases ileal magnesium solubility and apparent magnesium absorption in rats. , 1992, The Journal of nutrition.

[21]  P. Delmas,et al.  Biochemical markers of bone turnover: methodology and clinical use in osteoporosis. , 1991, The American journal of medicine.

[22]  A. Pointillart.  Enhancement of phosphorus utilization in growing pigs fed phytate-rich diets by using rye bran. , 1991, Journal of animal science.

[23]  E. Kornegay,et al.  Calcium and phosphorus requirement of swine from weaning to market weight: I. Development of response curves for performance. , 1991, Journal of animal science.

[24]  J. H. Wilson,et al.  Calcium and phosphorus requirement of swine from weaning to market weight: II. Development of response curves for bone criteria and comparison of bending and shear bone testing. , 1991, Journal of animal science.

[25]  G. Breves,et al.  Comparative Aspects of Gastrointestinal Phosphorus Metabolism , 1991, Nutrition Research Reviews.

[26]  N. Brautbar,et al.  Magnesium absorption: mechanisms and the influence of vitamin D, calcium and phosphate. , 1991, The Journal of nutrition.

[27]  H. DeLuca,et al.  The vitamin D system: 1990. , 1990, Kidney international. Supplement.

[28]  A. Norman.  The avian as an animal model for the study of the vitamin D endocrine system. , 1990, The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry.

[29]  H. Lüdke,et al.  [The phosphorus supply of pigs in the body weight category of 11-38 kg. 1. The effect of the phosphorus supply on the performance, nutrient digestibility, protein and fat content in soft tissue and skeleton and protein and fat deposits]. , 1989, Archiv fur Tierernahrung.