Resolution enhancement of protein PMR spectra using the difference between a broadened and a normal spectrum

Abstract Methods are described which improve the resolution of a complex NMR spectrum by subtracting a broadened spectrum from the original one. The broadening is brought about either by multiplying a transient response by an exponentially decaying function or by introducing a paramagnetic ion. The methods are analysed and are illustrated using the 270 MHz spectra of human and hen egg white lysozymes. The improvement in resolution is such that relaxation and shift measurements may be performed in regions of the spectrum previously thought inaccessible.

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