Comparison of 864 MHz and 935 MHz microwave radiation effects on cell culture.

The objective of this study was to compare the effects of 864 MHz and 935 MHz radiofrequency/microwave radiation on the ability of V79 cells to proliferate, form colonies and on their viability. For one, two and three hours, the cells were exposed to the 864 MHz field in a transversal electromagnetic mode cell (TEM) connected with amplifier and to the 935 MHz field in a gigahertz transversal electromagnetic mode cell (GTEM) equipped with a signal generator. The average specific absorption rate (SAR) was 0.08 W kg(-1) for the 864 MHz field and 0.12 W kg(-1) for the 935 MHz field. In comparison to the control cell samples, the growth curve of the 864 MHz irradiated cells showed a significant decrease after two-hour and three-hour exposure on the Day 3 after exposure. Likewise, cells exposed to 935 MHz microwaves for three hours showed a significant growth on Day 3 after exposure. The colony-forming ability and viability of cells exposed to 864 MHz and 935 MHz microwaves did not significantly differ from the matched controls. The applied RF/MW fields showed a similar effect on cell culture growth, colony-forming ability and viability of V79 cells.

[1]  I. Pavičić,et al.  Blood-forming system in rats after whole-body microwave exposure; reference to the lymphocytes. , 2004, Toxicology letters.

[2]  B. Modlic,et al.  Investigation of the genotoxic effect of microwave irradiation in rat bone marrow cells: in vivo exposure. , 2004, Mutagenesis.

[3]  Samuel Milham,et al.  Epidemiological studies of radio frequency exposures and human cancer. , 2004, Bioelectromagnetics.

[4]  A. Sannino,et al.  Lack of Genotoxic Effects (Micronucleus Induction) in Human Lymphocytes Exposed In Vitro to 900 MHz Electromagnetic Fields , 2003, Radiation research.

[5]  V. Kašuba,et al.  Micronucleus induction after whole-body microwave irradiation of rats. , 2002, Mutation research.

[6]  E. Moros,et al.  Radiofrequency Electromagnetic Fields do not Alter the Cell Cycle Progression of C3H 10T and U87MG Cells , 2001, Radiation research.

[7]  J. Mathieu,et al.  Non-thermal effects of continuous 2.45 GHz microwaves on Fas-induced apoptosis in human Jurkat T-cell line. , 2000, Bioelectrochemistry.

[8]  W F Pickard,et al.  Proto-oncogene mRNA levels and activities of multiple transcription factors in C3H 10T 1/2 murine embryonic fibroblasts exposed to 835.62 and 847.74 MHz cellular phone communication frequency radiation. , 1999, Radiation research.

[9]  P Raskmark,et al.  The effects of radiofrequency fields on cell proliferation are non-thermal. , 1999, Bioelectrochemistry and bioenergetics.

[10]  D. R. McKenzie,et al.  Electromagnetic radiation at 835 MHz changes the morphology and inhibits proliferation of a human astrocytoma cell line , 1997 .

[11]  Stephen F. Cleary,et al.  EFFECTS OF ISOTHERMAL 2.45 GHZ MICROWAVE RADIATION ON THE MAMMALIAN CELL CYCLE : COMPARISON WITH EFFECTS OF ISOTHERMAL 27 MHZ RADIOFREQUENCY RADIATION EXPOSURE , 1996 .

[12]  F. Keilmann,et al.  Nonthermal Effects of Millimeter Microwaves on Yeast Growth , 1978, Zeitschrift fur Naturforschung. Section C, Biosciences.

[13]  I. Trošić,et al.  Frequency of Micronucleated Erythrocytes in Rat Bone Marrow Exposed to 2.45?GHz Radiation , 2005 .

[14]  R. Ian Fresh,et al.  Culture of animal cells : a manual of basic technique , 2005 .

[15]  I. Trošić,et al.  Erythropoietic changes in rats after 2.45 GJz nonthermal irradiation. , 2004, International journal of hygiene and environmental health.

[16]  Juraj Bartolic,et al.  Design of a TEM-Cell with Increased Usable Test Area , 2003 .

[17]  M. Gulisano,et al.  Exposure to global system for mobile communication (GSM) cellular phone radiofrequency alters gene expression, proliferation, and morphology of human skin fibroblasts. , 2002, Oncology research.

[18]  P. Raskmark,et al.  Changes in cell proliferation due to environmental non-ionizing radiation: 2. Microwave radiation , 1998 .

[19]  W. R. Adey,et al.  DNA synthesis and cell proliferation in C6 glioma and primary glial cells exposed to a 836.55 MHz modulated radiofrequency field. , 1997, Bioelectromagnetics.

[20]  D Krause,et al.  Role of modulation on the effect of microwaves on ornithine decarboxylase activity in L929 cells. , 1997, Bioelectromagnetics.

[21]  G. Pedersen,et al.  FDTD Calulations of the EM-field Distribution in a Microtiter Suspension Well , 1995 .

[22]  C. Blackman,et al.  Dose dependence of acetylcholinesterase activity in neuroblastoma cells exposed to modulated radio-frequency electromagnetic radiation. , 1992, Bioelectromagnetics.