DNA optical sensor: a rapid method for the detection of DNA hybridization.

A DNA optical sensor system is proposed based on the combination of sandwich solution hybridization, magnetic bead capture, flow injection and chemiluminescence for rapid detection of DNA hybridization. Bacterial alkaline phosphatase (phoA) gene and Hepatitis B virus (HBV) DNA were used as target DNA. A biotinylated DNA probe was used to capture the target gene onto the streptavidin-coated magnetic beads and a calf intestine alkaline phosphatase (CAP)-labelled DNA probe was used for subsequent enzymatic chemiluminescence detection. The detection cycle was less than 30 min, excluding the DNA hybridization time, which was about 100 min. Both the phoA gene and HBV DNA could be detected at picogramme or femtomole level. No response signal was obtained when target DNA did not exist in the sample. Successive sample detection could be made by removing the magnetic field and a washing step.

[1]  Mary Lynn Grayeski,et al.  Flow-injection chemiluminescent method for an enzyme-labelled DNA probe , 1991 .

[2]  Yoshio Ishimori,et al.  Novel DNA sensor for electrochemical gene detection , 1994 .

[3]  P. Piunno,et al.  Fiber-optic DNA sensor for fluorometric nucleic acid determination. , 1995, Analytical chemistry.

[4]  S Beck,et al.  Chemiluminescent detection of DNA: application for DNA sequencing and hybridization. , 1989, Nucleic acids research.

[5]  E. Paleček,et al.  Cyclic voltammetry of submicrogram quantities of supercoiled, linear and denatured DNAs with DNA-modified mercury electrode. , 1993, Journal of biomolecular structure & dynamics.

[6]  P E Stanley,et al.  Chemiluminescent and bioluminescent reporter gene assays. , 1994, Analytical biochemistry.

[7]  M. Urdea,et al.  A novel method for the rapid detection of specific nucleotide sequences in crude biological samples without blotting or radioactivity; application to the analysis of hepatitis B virus in human serum. , 1987, Gene.

[8]  M. Wilchek,et al.  Nonradioactive hybridization probes prepared by the reaction of biotin hydrazide with DNA. , 1987, Biochemical and biophysical research communications.

[9]  Y. Vlasov,et al.  Surface plasmon resonance monitoring by means of polarization state measurement in reflected light as the basis of a DNA-probe biosensor , 1996 .

[10]  R. Weiss,et al.  Line scanning system for direct digital chemiluminescence imaging of DNA sequencing blots. , 1993, Analytical chemistry.

[11]  H. Söderlund,et al.  Fast quantification of nucleic acid hybrids by affinity-based hybrid collection. , 1986, Nucleic acids research.

[12]  Liang-Chy Chien,et al.  Nucleic Acid Hybridization Detected by Piezoelectric Resonance , 1988 .

[13]  W. Quint,et al.  Rapid detection of hepatitis C virus RNA by direct capture from blood , 1994, Journal of medical virology.

[14]  C. Kurz,et al.  A colorimetric method for DNA hybridization. , 1984, Nucleic acids research.

[15]  S C Huang,et al.  Binding of biotinylated DNA to streptavidin-coated polystyrene latex. , 1994, Analytical biochemistry.

[16]  P. Wilding,et al.  Imaging of chemiluminescent reactions in mesoscale silicon-glass microstructures. , 1994, Journal of bioluminescence and chemiluminescence.

[17]  J. Greve,et al.  Nucleic acid detection with surface plasmon resonance using cationic latex , 1994 .

[18]  A. Palva,et al.  Sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples. , 1983, Gene.

[19]  I. Bronstein,et al.  Imaging of chemiluminescent signals with cooled CCD camera systems. , 1994, Journal of bioluminescence and chemiluminescence.

[20]  Michael B. Thompson,et al.  Interfacial Nucleic Acid Hybridization Studied by Random Primer 32P Labeling and Liquid-Phase Acoustic Network Analysis , 1994 .

[21]  K. M. Millan,et al.  Voltammetric DNA biosensor for cystic fibrosis based on a modified carbon paste electrode. , 1994, Analytical chemistry.