Soluble CD14 is a nonspecific marker of monocyte activation

Soluble CD14 is associated with morbidity and mortality in HIV disease. It is a co-receptor for lipopolysaccharide (LPS) that is released from monocytes upon activation. We demonstrate here, that inflammatory cytokines can induce the release of sCD14 in peripheral blood mononuclear cell cultures from healthy donors, and that TLR ligands other than LPS can cause a decrease in the monocyte cell surface expression of CD14. Thus, sCD14 is a marker of monocyte activation, not restricted to activation by LPS.

[1]  P. Thompson,et al.  Modulatory effects of sCD14 and LBP on LPS-host cell interactions , 2005, Journal of endotoxin research.

[2]  E. Ruiz-Mateos,et al.  Long-term suppressive combined antiretroviral treatment does not normalize the serum level of soluble CD14. , 2013, The Journal of infectious diseases.

[3]  A. Tomasz,et al.  CD14 is a pattern recognition receptor. , 1994, Immunity.

[4]  Handan Wand,et al.  Plasma levels of soluble CD14 independently predict mortality in HIV infection. , 2011, The Journal of infectious diseases.

[5]  Ping-yuan Wang,et al.  Phosphatidylinositides Bind to Plasma Membrane CD14 and Can Prevent Monocyte Activation by Bacterial Lipopolysaccharide* , 1998, The Journal of Biological Chemistry.

[6]  M. Lederman,et al.  Soluble markers of inflammation and coagulation but not T-cell activation predict non-AIDS-defining morbid events during suppressive antiretroviral treatment. , 2014, The Journal of infectious diseases.

[7]  P. Poole‐Wilson,et al.  Elevated soluble CD14 receptors and altered cytokines in chronic heart failure. , 1997, The American journal of cardiology.

[8]  J. Strominger,et al.  Shedding as a mechanism of down-modulation of CD14 on stimulated human monocytes. , 1991, Journal of immunology.

[9]  Martin Lacher,et al.  Expression, regulation and clinical significance of soluble and membrane CD14 receptors in pediatric inflammatory lung diseases , 2010, Respiratory research.

[10]  M. Lederman,et al.  Immunologic failure despite suppressive antiretroviral therapy is related to activation and turnover of memory CD4 cells. , 2011, The Journal of infectious diseases.

[11]  C. Eckerskorn,et al.  Soluble lipopolysaccharide receptor (CD14) is released via two different mechanisms from human monocytes and CD14 transfectants , 1995, European journal of immunology.

[12]  H. Engelmann,et al.  The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms. , 1996, European journal of biochemistry.

[13]  S. Frøland,et al.  Elevated levels of serum-soluble CD14 in human immunodeficiency virus type 1 (HIV-1) infection: correlation to disease progression and clinical events. , 1998, Blood.

[14]  M. Lederman,et al.  Gut epithelial barrier dysfunction and innate immune activation predict mortality in treated HIV infection. , 2014, The Journal of infectious diseases.

[15]  O. Yang,et al.  Biomarkers of microbial translocation and macrophage activation: association with progression of subclinical atherosclerosis in HIV-1 infection. , 2012, The Journal of infectious diseases.

[16]  P. Libby,et al.  Cutting Edge: Heat Shock Protein (HSP) 60 Activates the Innate Immune Response: CD14 Is an Essential Receptor for HSP60 Activation of Mononuclear Cells1 , 2000, The Journal of Immunology.

[17]  J. Brenchley,et al.  Microbial translocation is a cause of systemic immune activation in chronic HIV infection , 2006, Retrovirology.

[18]  R. Ulevitch,et al.  CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. , 1990, Science.

[19]  S. Wright,et al.  Lipopolysaccharide binding protein and soluble CD14 catalyze exchange of phospholipids. , 1997, The Journal of clinical investigation.

[20]  C. Gabay,et al.  CD14 Is an Acute-Phase Protein1 , 2004, The Journal of Immunology.

[21]  D. Douek,et al.  Host response to translocated microbial products predicts outcomes of patients with HBV or HCV infection. , 2011, Gastroenterology.

[22]  P. Ferrara,et al.  The two soluble forms of the lipopolysaccharide receptor, CD14: Characterization and release by normal human monocytes , 1994, European journal of immunology.

[23]  M. Lederman,et al.  Plasma Proteome Analysis Reveals Overlapping, yet Distinct Mechanisms of Immune Activation in Chronic HCV and HIV Infections , 2013, Journal of acquired immune deficiency syndromes.