Characterization of oil-producing microalgae using Raman spectroscopy

Raman spectroscopy offers a powerful alterna- tive analytical method for the detection and identification of lipids/oil in biological samples, such as algae and fish. Recent research in the authors' groups, and experimental data only very recently published by us and a few other groups suggest that Raman spectroscopy can be exploited in instances where fast and accurate determination of the iodine value (associated with the degree of lipid unsaturation) is required. Here the cur- rent status of Raman spectroscopy applications on algae is re- viewed, and particular attention is given to the efforts of identi- fying and selecting oil-rich algal strains for the potential mass production of commercial biofuels and for utilization in the food industry. Normalized intensity, a.u.