Electrostatically mediated liposome fusion and lipid exchange with a nanoparticle-supported bilayer for control of surface charge, drug containment, and delivery.

The loading and containment of cargo within nanoparticles and their efficient delivery to cells represent a primary challenge in nanomedicine. We report lipid exchange between free and mesoporous silica nanoparticle-supported lipid bilayers as an effective means of containing cargo, controlling charge, and directing delivery to mammalian cells. The delivery of a membrane-impermeable dye (calcein) and a chemotherapeutic drug (doxorubicin) are demonstrated. Exchanged lipid bilayers minimized premature drug release, and an overall positive charge on the supported lipid bilayer effected enhanced delivery.

[1]  C. Jeffrey Brinker,et al.  Porous nanoparticle supported lipid bilayers (protocells) as delivery vehicles. , 2009, Journal of the American Chemical Society.

[2]  T. Bayerl,et al.  Lipid transfer between small unilamellar vesicles and single bilayers on a solid support: self-assembly of supported bilayers with asymmetric lipid distribution. , 1994, Biochemistry.

[3]  V. S. Lin,et al.  Gatekeeping layer effect: a poly(lactic acid)-coated mesoporous silica nanosphere-based fluorescence probe for detection of amino-containing neurotransmitters. , 2004, Journal of the American Chemical Society.

[4]  T. Bein,et al.  Click chemistry for high-density biofunctionalization of mesoporous silica. , 2008, Journal of the American Chemical Society.

[5]  Plamen Atanassov,et al.  Photoregulation of Mass Transport through a Photoresponsive Azobenzene-Modified Nanoporous Membrane , 2004 .

[6]  Masahiro Fujiwara,et al.  Photocontrolled reversible release of guest molecules from coumarin-modified mesoporous silica , 2003, Nature.

[7]  R. Davis,et al.  Nanoporous microbead supported bilayers: stability, physical characterization, and incorporation of functional transmembrane proteins. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[8]  Brian G. Trewyn,et al.  Mesoporous Silica Nanoparticles for Drug Delivery and Biosensing Applications , 2007 .

[9]  Yuma Yamada,et al.  Multifunctional envelope-type nano device (MEND) as a non-viral gene delivery system. , 2008, Advanced drug delivery reviews.

[10]  Yunfeng Lu,et al.  Aerosol-assisted self-assembly of mesostructured spherical nanoparticles , 1999, Nature.

[11]  W. Ducker,et al.  Flip-flop in adsorbed bilayers. , 2006, The journal of physical chemistry. B.

[12]  J. Groves,et al.  Electrostatically Targeted Intermembrane Lipid Exchange with Micropatterned Supported Membranes , 2003 .

[13]  V. S. Lin,et al.  Mesoporous silica nanoparticles deliver DNA and chemicals into plants. , 2007, Nature nanotechnology.

[14]  Radostin Danev,et al.  Multi-layered nanoparticles for penetrating the endosome and nuclear membrane via a step-wise membrane fusion process. , 2009, Biomaterials.

[15]  C. Brinker,et al.  Optical detection of ion-channel-induced proton transport in supported phospholipid bilayers. , 2007, Nano letters.

[16]  E. Sackmann,et al.  Supported Membranes: Scientific and Practical Applications , 1996, Science.

[17]  R. Langer,et al.  Drug delivery and targeting. , 1998, Nature.

[18]  Chad A Mirkin,et al.  Nanostructures in biodiagnostics. , 2005, Chemical reviews.

[19]  K. Ariga.  Silica-supported biomimetic membranes. , 2004, Chemical record.

[20]  J. Israelachvili,et al.  Correlation of AFM and SFA measurements concerning the stability of supported lipid bilayers. , 2004, Biophysical journal.

[21]  Victor S-Y Lin,et al.  A mesoporous silica nanosphere-based carrier system with chemically removable CdS nanoparticle caps for stimuli-responsive controlled release of neurotransmitters and drug molecules. , 2003, Journal of the American Chemical Society.

[22]  B. Kasemo,et al.  Real-time QCM-D monitoring of electrostatically driven lipid transfer between two lipid bilayer membranes. , 2008, The journal of physical chemistry. B.

[23]  Françoise Brochard-Wyart,et al.  Vesicles surfing on a lipid bilayer: Self-induced haptotactic motion , 2006, Proceedings of the National Academy of Sciences.

[24]  María Vallet-Regí,et al.  Mesoporous materials for drug delivery. , 2007, Angewandte Chemie.

[25]  Kristen N. Duthie,et al.  Wide varieties of cationic nanoparticles induce defects in supported lipid bilayers. , 2008, Nano letters.

[26]  C. Ladavière,et al.  An overview of lipid membrane supported by colloidal particles. , 2007, Advances in colloid and interface science.

[27]  Jeffrey I. Zink,et al.  Photo-Driven Expulsion of Molecules from Mesostructured Silica Nanoparticles , 2007 .

[28]  Victor S-Y Lin,et al.  Stimuli-responsive controlled-release delivery system based on mesoporous silica nanorods capped with magnetic nanoparticles. , 2005, Angewandte Chemie.

[29]  Hsian-Rong Tseng,et al.  A reversible molecular valve. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[30]  Yajun Wang,et al.  Enzyme encapsulation in nanoporous silica spheres. , 2004, Chemical communications.

[31]  Monty Liong,et al.  Mesoporous silica nanoparticles as a delivery system for hydrophobic anticancer drugs. , 2007, Small.

[32]  Victor S-Y Lin,et al.  Mesoporous silica nanoparticles for intracellular delivery of membrane-impermeable proteins. , 2007, Journal of the American Chemical Society.

[33]  É. Duguet,et al.  The formation of supported lipid bilayers on silica nanoparticles revealed by cryoelectron microscopy. , 2005, Nano letters.

[34]  Matthias Epple,et al.  Inorganic nanoparticles as carriers of nucleic acids into cells. , 2008, Angewandte Chemie.