Comparison of Peptidic and Nonpeptidic (cid:1) -Opioid Agonists on Guanosine 5 (cid:1) - O -(3-[ 35 S]thio)triphosphate ([ 35 S]GTP (cid:2) S) Binding in Brain Slices from Sprague-Dawley Rats

Previous studies have demonstrated that peptidic and nonpep- tidic (cid:1) -opioid receptor agonists have different effects depending on the measure. For example, nonpeptidic (cid:1) -opioid agonists, but not peptidic agonists, produce convulsions in rats, and in vitro studies suggested that peptidic and nonpeptidic (cid:1) -opioid agonists might have differential mechanisms of receptor down- regulation. The present study evaluated potential differences between peptidic and nonpeptidic (cid:1) -opioid agonists in their ability to activate G proteins using guanosine 5 (cid:1) - O -(3-[ 35 S]thio)triphos-phate ([ 35 S]GTP (cid:2) S) autoradiography experiments in rat brain slices. The peptidic agonist [ D -Pen 2 , D -Pen 5 ]-enkephalin and the nonpeptidic agonist ( (cid:2) )BW373U86 [( (cid:2)

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