Ameliorative effect of parsley oil on cisplatin-induced hepato-cardiotoxicity: A biochemical, histopathological, and immunohistochemical study.

Cisplatin (cis-diamminedichloroplatinum, CDDP) is an effective DNA alkylating agent used in the treatment of different types of tumors; however, its clinical use is associated with hepato-cardiotoxicity. The current study was designed to assess the potential protective effect of parsley oil (PO) against CDDP-induced hepato-cardiotoxicity. For this purpose, 25 adult male rats were assigned into five groups, each containing five animals. Group I (control) was administered saline solution. Group II was administered PO at a dosage of 0.42ml/kg BW. Group III were administered CDDP at a dosage of 5mg/kg BW. Group IV was administered PO in addition to CDDP. Group V was administered saline solution in addition to CDDP, after which they were administered PO for five days. Oral administration of either saline solution or PO was performed each day for 10days, while administration of CDDP was via a single intraperitoneal injection five days following the commencement of the experiment. The recorded results revealed that CDDP induced obvious hepatic and cardiac injuries that were indicated by biochemical, histopathological, and immunohistochemical alterations, including elevation of serum hepatic and cardiac injury markers as well as proinflammatory cytokines. Moreover, CDDP induced an increase in the level of hepatic and cardiac injury biomarkers, decreases in the activities of antioxidant enzymes, a decrease in GSH concentration, and an increase in MDA concentration. CDDP also induced histopathological hepatocellular and myocardial changes, and overexpression of p53 and COX-2 in hepatic and cardiac tissues. Administration of PO either as a preventative medicine or as treatment significantly improved all the observed deleterious effects induced by CDDP in rat liver and heart. Thus, it may be concluded that PO, with its antioxidant, anti-inflammatory, and antiapoptotic activities, can potentially be used in the treatment of CDDP-induced hepatic and cardiac injuries.

[1]  W B Jakoby,et al.  Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. , 1974, The Journal of biological chemistry.

[2]  John A. Kiernan,et al.  Histological and Histochemical Methods: Theory and Practice, 4th edition , 2008 .

[3]  M. Uchiyama,et al.  Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. , 1978, Analytical biochemistry.

[4]  M. Farzaei,et al.  Parsley: a review of ethnopharmacology, phytochemistry and biological activities. , 2013, Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan.

[5]  A. Telser HISTOLOGICAL AND HISTOCHEMICAL METHODS: THEORY AND PRACTICE, 3rd EDITION , 1999 .

[6]  S REITMAN,et al.  A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. , 1957, American journal of clinical pathology.

[7]  Z. Yıldırım,et al.  The effects of erdosteine on the activities of some metabolic enzymes during cisplatin-induced nephrotoxicity in rats. , 2004, Pharmacological research.

[8]  M. Abdel-Daim,et al.  Alpha-lipoic acid improves acute deltamethrin-induced toxicity in rats. , 2014, Canadian journal of physiology and pharmacology.

[9]  J. Błasiak,et al.  Cisplatin-evoked DNA fragmentation in normal and cancer cells and its modulation by free radical scavengers and the tyrosine kinase inhibitor STI571. , 2004, Chemico-biological interactions.

[10]  N. Mimica-Dukić,et al.  Effect of parsley (Petroselinum crispum (Mill.) Nym. ex A.W. Hill, Apiaceae) extracts on some biochemical parameters of oxidative stress in mice treated with CCl4 , 2007, Phytotherapy research : PTR.

[11]  M. Abdel-Daim,et al.  Trigonella foenum-graecum protection against deltamethrin-induced toxic effects on haematological, biochemical, and oxidative stress parameters in rats. , 2014, Canadian journal of physiology and pharmacology.

[12]  T. Ozben Oxidative stress and apoptosis: impact on cancer therapy. , 2007, Journal of pharmaceutical sciences.

[13]  R. Sistla,et al.  Ameliorative Effect of Fisetin on Cisplatin-Induced Nephrotoxicity in Rats via Modulation of NF-κB Activation and Antioxidant Defence , 2014, PloS one.

[14]  S. Rashid,et al.  Alleviation of hepatic injury by chrysin in cisplatin administered rats: Probable role of oxidative and inflammatory markers , 2014, Pharmacological reports : PR.

[15]  A. Al-Majed Carnitine deficiency provokes cisplatin-induced hepatotoxicity in rats. , 2007, Basic & clinical pharmacology & toxicology.

[16]  J. Waldenström,et al.  Creatine kinase in serum: 6. Inhibition by endogenous polyvalent cations, and effect of chelators on the activity and stability of some assay components. , 1979, Clinical chemistry.

[17]  John A. Kiernan,et al.  Histological and Histochemical Methods: Theory and Practice , 1981 .

[18]  R. Safirstein,et al.  Cisplatin nephrotoxicity. , 2003, Seminars in nephrology.

[19]  E. Mimnaugh,et al.  Enhancement of reactive oxygen-dependent mitochondrial membrane lipid peroxidation by the anticancer drug adriamycin. , 1985, Biochemical pharmacology.

[20]  B. Stoner CLINICAL INFECTIOUS DISEASES: A PRACTICAL APPROACH , 1999 .

[21]  Abdelazim Ibrahim,et al.  Nephroprotective effect of bee honey and royal jelly against subchronic cisplatin toxicity in rats , 2016, Cytotechnology.

[22]  D. Abo-Elmatty,et al.  Cisplatin-induced cardiotoxicity: Mechanisms and cardioprotective strategies. , 2011, European journal of pharmacology.

[23]  J. Bancroft,et al.  Bancroft's Theory and Practice of Histological Techniques , 2018 .

[24]  M. Cilli,et al.  Morphological and histochemical evidence of the protective effect of procainamide hydrochloride on tissue damage induced by repeated administration of low doses of cisplatin. , 2005, Anticancer research.

[25]  C. Gartner,et al.  Protein and nonprotein cysteinyl thiol modification by N-acetyl-p-benzoquinone imine via a novel ipso adduct. , 1999, Biochemistry.

[26]  B. Halliwell Reactive Species and Antioxidants. Redox Biology Is a Fundamental Theme of Aerobic Life , 2006, Plant Physiology.

[27]  E. Beutler,et al.  Improved method for the determination of blood glutathione. , 1963, The Journal of laboratory and clinical medicine.

[28]  H. Aebi,et al.  Catalase in vitro. , 1984, Methods in enzymology.

[29]  A. Nasr,et al.  Aged garlic extract protects against oxidative stress and renal changes in cisplatin-treated adult male rats , 2014, Cancer Cell International.

[30]  S. Trivić,et al.  Antioxidant Activities of Celery and Parsley Juices in Rats Treated with Doxorubicin , 2010, Molecules.

[31]  A. Hassan,et al.  Trigonella foenum-graecum ameliorates acrylamide-induced toxicity in rats: Roles of oxidative stress, proinflammatory cytokines, and DNA damage. , 2015, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[32]  A. C. Santos,et al.  Cisplatin induces mitochondrial oxidative stress with resultant energetic metabolism impairment, membrane rigidification and apoptosis in rat liver , 2008, Journal of applied toxicology : JAT.

[33]  Roger Y. Tsang,et al.  Cisplatin Overdose , 2009, Drug safety.

[34]  S. Buhl,et al.  Optimal conditions and comparison of lactate dehydrogenase catalysis of the lactate-to-pyruvate and pyruvate-to-lactate reactions in human serum at 25, 30, and 37 degrees C. , 1978, Clinical chemistry.

[35]  Y. El-Sayed,et al.  Synergistic ameliorative effects of sesame oil and alpha-lipoic acid against subacute diazinon toxicity in rats: hematological, biochemical, and antioxidant studies. , 2016, Canadian journal of physiology and pharmacology.

[36]  野口 照久 「Evaluation of Drug Activities: Pharmacometrics」, Vol. 1, 15×23cm判, 456頁, 5,700円, Vol.2 438頁, 5,400円, Academic Press, D. R. Laurence & A. L. Bacharach編 , 1965 .

[37]  Feng Chen,et al.  Evaluation of antioxidant activity of parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents , 2006 .

[38]  Jeffrey W. Prichard,et al.  Handbook of Practical Immunohistochemistry , 2011 .

[39]  W. Valentine,et al.  Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. , 1967, The Journal of laboratory and clinical medicine.

[40]  K. Yagi,et al.  The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. , 1972, Biochemical and biophysical research communications.

[41]  G. Şener,et al.  Effects of parsley (Petroselinum crispum) on the aorta and heart of Stz induced diabetic rats , 2003 .

[42]  M. Abdel-Daim,et al.  Iranian Journal of Basic Medical Sciences Effects of Nigella sativa oil and ascorbic acid against oxytetracycline-induced hepato-renal toxicity in rabbits , 2022 .

[43]  C. Petitclerc,et al.  A reference method for measurement of alkaline phosphatase activity in human serum. , 1983, Clinical chemistry.

[44]  S. Lippard,et al.  Specific binding of chromosomal protein HMG1 to DNA damaged by the anticancer drug cisplatin. , 1992, Science.

[45]  W. Prellwitz,et al.  Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity. Activity kinetics and diagnostic significance in myocardial infarction. , 1976, Clinica chimica acta; international journal of clinical chemistry.

[46]  Salim S. Al-Rejaie,et al.  Propionyl‐L‐carnitine prevents The Progression of Cisplatin‐Induced Cardiomyopathy in a Carnitine‐Depleted Rat Model , 2006, Pharmacological research.

[47]  Aziza M. Hassan,et al.  Antioxidant effect of parsley and panax ginseng extract standardized with ginsenosides Rg3 against alteration induced in reproductive functions in male mice , 2006 .