Extensive junctional diversity of rearranged human T cell receptor delta genes.

The human T cell receptor delta (TCR delta) gene encodes one component of the TCR gamma delta-CD3 complex found on subsets of peripheral blood and thymic T cells. Human TCR delta diversity was estimated by characterizing rearrangements in TCR gamma delta cell lines and determining the structures of complementary DNA clones representing functional and nonfunctional transcripts in these cell lines. One V delta segment and one J delta segment were identified in all functional transcripts, although a distinct J delta segment was identified in a truncated transcript. Further, one D delta element was identified, and evidence for the use of an additional D delta element was obtained. Thus human TCR delta genes appear to use a limited number of germline elements. However, the apparent use of two D delta elements in tandem coupled with imprecise joining and extensive incorporation of N nucleotides generates unprecedented variability in the junctional region.

[1]  Mark M. Davis,et al.  T-cell receptor δ gene rearrangements in early thymocytes , 1987, Nature.

[2]  J. Freed,et al.  Peptide sequences of T-cell receptor δ and γ chains are identical to predicted X and γ proteins , 1987, Nature.

[3]  Mark M. Davis,et al.  Identification and sequence of a fourth human T cell antigen receptor chain , 1987, Nature.

[4]  J. Bluestone,et al.  Major histocompatibility complex-linked specificity of γδ receptor-bearing T lymphocytes , 1987, Nature.

[5]  M. Krangel,et al.  Identification of putative human T cell receptor delta complementary DNA clones. , 1987, Science.

[6]  J. Mclean,et al.  Immunochemical proof that a novel rearranging gene encodes the T cell receptor delta subunit. , 1987, Science.

[7]  J. Seidman,et al.  Measuring the human T cell receptor gamma-chain locus. , 1987, Science.

[8]  J. Mclean,et al.  Structurally divergent human T cell receptor gamma proteins encoded by distinct C gamma genes. , 1987, Science.

[9]  C. Richardson,et al.  DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[10]  B. Alarcón,et al.  The T-cell receptor gamma chain-CD3 complex: implication in the cytotoxic activity of a CD3+ CD4- CD8- human natural killer clone. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Mark M. Davis,et al.  A new T-cell receptor gene located within the alpha locus and expressed early in T-cell differentiation , 1987, Nature.

[12]  J. Coligan,et al.  Identification of a T3-associated gamma delta T cell receptor on Thy-1+ dendritic epidermal Cell lines. , 1987, Science.

[13]  J. Seidman,et al.  Functional gamma chain-associated T cell receptors on cerebrospinal fluid-derived natural killer-like T cell clones , 1987, The Journal of experimental medicine.

[14]  J. Seidman,et al.  Human T cell gamma chain joining regions and T cell development. , 1987, Journal of immunology.

[15]  N. Federspiel,et al.  The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8- T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer , 1987, The Journal of experimental medicine.

[16]  J. Seidman,et al.  Characterization of an expressed CDS-associated Ti γ-chain reveals Cγ domain polymorphism , 1987, Nature.

[17]  J. Coligan,et al.  Differential expression of two distinct T-cell receptors during thymocyte development , 1987, Nature.

[18]  F. Faure,et al.  A γ-chain complex forms a functional receptor on cloned human lymphocytes with natural killer-like activity , 1987, Nature.

[19]  J. Seidman,et al.  Two forms of the T-cell receptor γ protein found on peripheral blood cytotoxic T lymphocytes , 1987, Nature.

[20]  J. Seidman,et al.  A T-cell receptor γ/CD3 complex found on cloned functional lymphocytes , 1987, Nature.

[21]  S. Tonegawa,et al.  Tγ protein is expressed on murine fetal thymocytes as a disulphide-linked heterodimer , 1987, Nature.

[22]  T. Mak,et al.  Genes of the T-cell antigen receptor in normal and malignant T cells. , 1987, Annual review of immunology.

[23]  J. Allison,et al.  Delta is the C x-gene product in the γ/δ antigen receptor of dendritic epidermal cells , 1987, Nature.

[24]  J. Coligan,et al.  Characterization of T cell receptor gamma chain expression in a subset of murine thymocytes. , 1986, Science.

[25]  M. Lefranc,et al.  Genetic polymorphism and exon changes of the constant regions of the human T-cell rearranging gene gamma. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[26]  F. Alt,et al.  A functional T3 molecule associated with a novel heterodimer on the surface of immature human thymocytes , 1986, Nature.

[27]  A. Weiss,et al.  Expression of T3 in association with a molecule distinct from the T-cell antigen receptor heterodimer. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[28]  J. Seidman,et al.  Human T-cell γ genes contain N segments and have marked junctional variability , 1986, Nature.

[29]  J. Seidman,et al.  Identification of a putative second T-cell receptor , 1986, Nature.

[30]  M. Lefranc,et al.  Diversity and rearrangement of the human T cell rearranging γ genes: Nine germ-line variable genes belonging to two subgroups , 1986, Cell.

[31]  L. Hood,et al.  The molecular genetics of the T-cell antigen receptor and T-cell antigen recognition. , 1986, Annual review of immunology.

[32]  P. Marrack,et al.  The antigen-specific, major histocompatibility complex-restricted receptor on T cells. , 1986, Advances in immunology.

[33]  L. Hood,et al.  Identification of a diversity segment of human T-cell receptor β-chain, and comparison with the analogous murine element , 1984, Nature.

[34]  Mark M. Davis,et al.  A third type of murine T-cell receptor gene , 1984, Nature.

[35]  S. Tonegawa,et al.  A third rearranged and expressed gene in a clone of cytotoxic T lymphocytes , 1984, Nature.

[36]  Mark M. Davis,et al.  Localization of a T-cell receptor diversity-region element , 1984, Nature.

[37]  Mark M. Davis,et al.  Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins , 1984, Nature.

[38]  Tak W. Mak,et al.  A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains , 1984, Nature.

[39]  J. Yagüe,et al.  Primary structure of human T-cell receptor α-chain , 1984, Nature.

[40]  L. Hood,et al.  The structure, rearrangement and expression of Dβ gene segments of the murine T-cell antigen receptor , 1984, Nature.

[41]  S. Tonegawa,et al.  Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences , 1984, Nature.

[42]  R. Germain,et al.  Monoclonal antibodies against the antigen receptor on a cloned T-cell hybrid. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[43]  A. Feinberg,et al.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. , 1983, Analytical biochemistry.

[44]  P. Marrack,et al.  The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody , 1983, The Journal of experimental medicine.

[45]  E. Reinherz,et al.  Clonotypic structures involved in antigen-specific human T cell function. Relationship to the T3 molecular complex , 1983, The Journal of experimental medicine.

[46]  J. Allison,et al.  Tumor-specific antigen of murine T-lymphoma defined with monoclonal antibody. , 1982, Journal of immunology.

[47]  F. Alt,et al.  Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[48]  L. Hood,et al.  An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH , 1980, Cell.

[49]  Hitoshi Sakano,et al.  Sequences at the somatic recombination sites of immunoglobulin light-chain genes , 1979, Nature.

[50]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.