Enzymatic saccharification of pretreated sunflower stalks

Abstract The sunflower stalks were pretreated by steam explosion (at 1.05 kg / cm 2 for 0.5– 1.5 h ) and sodium hydroxide (0.25– 1.5% w / v NaOH at 1.05 kg / cm 2 for 0.5– 1.5 h ) using solid:liquid ratio of 0.05 g / ml and subsequently saccharified enzymatically. Steam explosion at 1.05 kg / cm 2 pressure for 1.5 h was found to be the optimum pretreatment. Maximum enzymatic saccharification of 57.8% was observed by treating 5% ( w / v ) pretreated sunflower stalks with T. reesei Rut-C 30 cellulase ( 25 FPU / g ) at 50°C, pH 5.0 for 72 h .

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