Isolation and characterization of human osteoblasts from needle biopsies without in vitro culture

[1]  J. Seidman,et al.  An RNA‐seq protocol to identify mRNA expression changes in mouse diaphyseal bone: Applications in mice with bone property altering Lrp5 mutations , 2013, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[2]  Wei Li,et al.  RSeQC: quality control of RNA-seq experiments , 2012, Bioinform..

[3]  L. Bonewald,et al.  Isolation and culture of primary osteocytes from the long bones of skeletally mature and aged mice. , 2012, BioTechniques.

[4]  S. Khosla,et al.  Characterization of mesenchymal progenitor cells isolated from human bone marrow by negative selection. , 2012, Bone.

[5]  K. Hansen,et al.  Removing technical variability in RNA-seq data using conditional quantile normalization , 2012, Biostatistics.

[6]  Anita H. Undale,et al.  Effects of intermittent parathyroid hormone treatment on osteoprogenitor cells in postmenopausal women. , 2011, Bone.

[7]  S. Khosla,et al.  Effects of estrogen on osteoprogenitor cells and cytokines/bone-regulatory factors in postmenopausal women. , 2011, Bone.

[8]  T. Kohler,et al.  Trabecular bone microstructure and local gene expression in iliac crest biopsies of men with idiopathic osteoporosis , 2011, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[9]  A. Angelini,et al.  Widespread Increase in Myeloid Calcifying Cells Contributes to Ectopic Vascular Calcification in Type 2 Diabetes , 2011, Circulation research.

[10]  L. Lerman,et al.  Coronary endothelial dysfunction in humans is associated with coronary retention of osteogenic endothelial progenitor cells. , 2010, European heart journal.

[11]  G. Karsenty,et al.  The central regulation of bone mass, the first link between bone remodeling and energy metabolism. , 2010, The Journal of clinical endocrinology and metabolism.

[12]  Chao Wan,et al.  Insulin Receptor Signaling in Osteoblasts Regulates Postnatal Bone Acquisition and Body Composition , 2010, Cell.

[13]  R. DePinho,et al.  Insulin Signaling in Osteoblasts Integrates Bone Remodeling and Energy Metabolism , 2010, Cell.

[14]  Eric T. Wang,et al.  An Abundance of Ubiquitously Expressed Genes Revealed by Tissue Transcriptome Sequence Data , 2009, PLoS Comput. Biol..

[15]  Davis J. McCarthy,et al.  edgeR: a Bioconductor package for differential expression analysis of digital gene expression data , 2009, Bioinform..

[16]  Robin K Suda,et al.  Circulating Osteogenic Precursor Cells in Heterotopic Bone Formation , 2009, Stem cells.

[17]  L. Pachter,et al.  TopHat: discovering splice junctions with RNA-Seq , 2009, Bioinform..

[18]  Cole Trapnell,et al.  Ultrafast and memory-efficient alignment of short DNA sequences to the human genome , 2009, Genome Biology.

[19]  D. Hume,et al.  Osteal macrophages: a new twist on coupling during bone dynamics. , 2008, Bone.

[20]  S. Khosla,et al.  Osteocalcin expression by circulating endothelial progenitor cells in patients with coronary atherosclerosis. , 2008, Journal of the American College of Cardiology.

[21]  David A. Hume,et al.  Osteal Tissue Macrophages Are Intercalated throughout Human and Mouse Bone Lining Tissues and Regulate Osteoblast Function In Vitro and In Vivo1 , 2008, The Journal of Immunology.

[22]  H. K. Genant,et al.  Advanced CT bone imaging in osteoporosis , 2008, Rheumatology.

[23]  Joel S Greenberger,et al.  Age‐related intrinsic changes in human bone‐marrow‐derived mesenchymal stem cells and their differentiation to osteoblasts , 2008, Aging cell.

[24]  S. Majumdar.  Magnetic resonance imaging for osteoporosis , 2008, Skeletal Radiology.

[25]  M. Karsdal,et al.  An update on biomarkers of bone turnover and their utility in biomedical research and clinical practice , 2006, European Journal of Clinical Pharmacology.

[26]  J. Gustafsson,et al.  Cholesterol‐Sensing Receptors, Liver × Receptor α and β, Have Novel and Distinct Roles in Osteoclast Differentiation and Activation , 2006 .

[27]  Eivind Hovig,et al.  Options available for profiling small samples: a review of sample amplification technology when combined with microarray profiling , 2006, Nucleic acids research.

[28]  Sheng Zhao,et al.  Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction , 2005, J. Comput. Biol..

[29]  M. Herrler,et al.  Linear mRNA amplification from as little as 5 ng total RNA for global gene expression analysis. , 2004, BioTechniques.

[30]  W. Siegert,et al.  Guideline to reference gene selection for quantitative real-time PCR. , 2004, Biochemical and biophysical research communications.

[31]  E. Ritman,et al.  Cryostatic micro-computed tomography imaging of arterial wall perfusion. , 2002, Scanning.

[32]  F. Speleman,et al.  Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes , 2002, Genome Biology.

[33]  J. Katzmann,et al.  Isolation and preliminary characterization of a monoclonal antibody that interacts preferentially with the liver isoenzyme of human alkaline phosphatase. , 1985, Clinical chemistry.

[34]  R. Luben,et al.  Biochemical characterization with parathormone and calcitonin of isolated bone cells: provisional identification of osteoclasts and osteoblasts. , 1976, Endocrinology.

[35]  S. Khosla,et al.  Effects of bisphosphonate treatment on circulating osteogenic endothelial progenitor cells in postmenopausal women. , 2013, Mayo Clinic proceedings.

[36]  J. Gustafsson,et al.  Cholesterol-sensing receptors, liver X receptor alpha and beta, have novel and distinct roles in osteoclast differentiation and activation. , 2006, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[37]  J. Aubin,et al.  Advances in the osteoblast lineage. , 1998, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[38]  K. A. Johnson,et al.  Outpatient percutaneous biopsy of the iliac crest: methods, morbidity, and patient acceptance. , 1986, Mayo Clinic proceedings.