Modeling the tryptic hydrolysis of pea proteins using an artificial neural network

Abstract Experimentally determined values for the degree of hydrolysis (DH) were used with an artificial neural network (ANN) model to predict the tryptic hydrolysis of a commercially available pea protein isolate at temperatures of 40, 45, and 50 °C. Analyses were conducted using the STATISTICA Neural Networks software on a personal computer. Input data were randomized to two sets: learning and testing. Differences between the experimental and calculated DH% were slight and ranged from 0.06% to 0.24%. The performance of the educated ANN was then tested by inputting temperatures ranging from 35 to 50 °C. Very strong correlations were found between calculated DH% values obtained from the ANN and those experimentally determined at all temperatures; the determination coefficients (R2) varied from 0.9958 to 0.9997. The results so obtained will be useful to reduce the time required in the design of enzymatic reactions involving food proteins.

[1]  A. Buciński,et al.  Application of artificial neural networks for modelling pea protein hydrolysis by trypsin , 2004 .

[2]  R. Amarowicz,et al.  Determination of α-amino nitrogen in pea protein hydrolysates: a comparison of three analytical methods , 1998 .

[3]  Shyam S. Sablani,et al.  Using neural networks to predict thermal conductivity of food as a function of moisture content, temperature and apparent porosity , 2003 .

[4]  J. Adler-Nissen Control of the proteolytic reaction and of the level of bitterness in protein hydrolysis processes , 2008 .

[5]  José S. Torrecilla,et al.  A neural network approach for thermal/pressure food processing , 2004 .

[6]  S Vlassides,et al.  Using historical data for bioprocess optimization: modeling wine characteristics using artificial neural networks and archived process information. , 2001, Biotechnology and bioengineering.

[7]  Wladyslaw Kaminski,et al.  DEGRADATION OF ASCORBIC ACID IN DRYING PROCESS -A COMPARISON OF DESCRIPTION METHODS , 2000 .

[8]  Da-Wen Sun,et al.  Learning techniques used in computer vision for food quality evaluation: a review , 2006 .

[9]  Véronique Bellon-Maurel,et al.  Pattern analysis techniques to process fermentation curves: Application to discrimination of enological alcoholic fermentations , 2002, Biotechnology and bioengineering.

[10]  Hosahalli S. Ramaswamy,et al.  Modeling and optimization of variable retort temperature (VRT) thermal processing using coupled neural networks and genetic algorithms , 2002 .

[11]  A. Halasz,et al.  Methionine Enrichment of Milk Protein by Enzymatic Peptide Modification , 1988 .

[12]  Jan Van Impe,et al.  Evaluation of model parameter accuracy by using joint confidence regions: application to low complexity neural networks to describe enzyme inactivation , 1998 .

[13]  R. Aluko,et al.  Limited enzymatic proteolysis increases the level of incorporation of canola proteins into mayonnaise , 2005 .

[14]  S. Nakai,et al.  Application of artificial neural networks for predicting the thermal inactivation of bacteria: a combined effect of temperature, pH and water activity , 2001 .

[15]  J E Haugen,et al.  Electronic nose and artificial neural network. , 1998, Meat science.

[16]  Jan Van Impe,et al.  Modeling the kinetics of isobaric-isothermal inactivation of Bacillus subtilis α-amylase with artificial neural networks , 1997 .