Total internal reflection holographic microscopy for quantitative phase characterization of cellular adhesion

A new form of near-field microscopy is presented using digital holography for quantitative phase imagery and characterization of cell-substrate interfaces. This imaging technique, termed total internal reflection holographic microscopy (TIRHM), utilizes an evanescent wave phase shift from the presence of cellular organisms, membranes, adhesions, and tissue structures on a prism face in order to modulate an object beam wavefront in a digital holographic microscope. Quantitative phase images of live cellular specimens are presented.

[1]  J. Coates,et al.  Cell-cell adhesion and signal transduction during Dictyostelium development. , 2001, Journal of cell science.

[2]  Michael Spencer,et al.  Fundamentals of light microscopy , 1982 .

[3]  M. K. Kim,et al.  Tomographic three-dimensional imaging of a biological specimen using wavelength-scanning digital interference holography. , 2000, Optics express.

[4]  Daniel Carl,et al.  Parameter-optimized digital holographic microscope for high-resolution living-cell analysis. , 2004, Applied optics.

[5]  H. J. Tanke,et al.  Biomedical Light Microscopy , 1991, Springer Netherlands.

[6]  E. Cuche,et al.  Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. , 2005, Optics letters.

[7]  Myung K. Kim,et al.  Cellular imagery with total internal reflection holographic microscopy , 2009, BiOS.

[8]  J. Gass,et al.  Phase imaging without 2pi ambiguity by multiwavelength digital holography. , 2003, Optics letters.

[9]  Lingfeng Yu,et al.  Movies of cellular and sub-cellular motion by digital holographic microscopy , 2006, Biomedical engineering online.

[10]  D. A. Hanson,et al.  Focal adhesion kinase: in command and control of cell motility , 2005, Nature Reviews Molecular Cell Biology.

[11]  F. Zernike.  How I discovered phase contrast. , 1955, Science.

[12]  W Xu,et al.  Digital in-line holography for biological applications , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[13]  Kwan Jeong,et al.  Fourier-domain digital holographic optical coherence imaging of living tissue. , 2007, Applied optics.

[14]  F. Wyrowski,et al.  Fast calculation method for optical diffraction on tilted planes by use of the angular spectrum of plane waves. , 2003, Journal of the Optical Society of America. A, Optics, image science, and vision.

[15]  C. Gottardi,et al.  Cadherins and cancer: how does cadherin dysfunction promote tumor progression? , 2008, Oncogene.

[16]  Dennis Bray,et al.  Cell Movements: From Molecules to Motility , 1992 .

[17]  A Finizio,et al.  Angular spectrum method with correction of anamorphism for numerical reconstruction of digital holograms on tilted planes. , 2005, Optics express.

[18]  Myung K. Kim,et al.  Quantitative imaging of cellular adhesion by total internal reflection holographic microscopy. , 2009, Applied optics.

[19]  Adam Wax,et al.  Quantitative phase microscopy with asynchronous digital holography. , 2007, Optics express.

[20]  Thomas S. Huang,et al.  Digital Holography , 2003 .

[21]  Deborah Leckband,et al.  Mechanism and dynamics of cadherin adhesion. , 2006, Annual review of biomedical engineering.

[22]  Quantitative characterization of cellular adhesions with Total Internal Reflection Holographic Microscopy , 2010 .

[23]  Joanna Schmit,et al.  Spectrally resolved white-light phase-shifting interference microscopy for thickness-profile measurements of transparent thin film layers on patterned substrates. , 2006, Optics express.

[24]  Myung K. Kim,et al.  Digital holography of total internal reflection. , 2008, Optics express.

[25]  H. Verschueren,et al.  Interference reflection microscopy in cell biology: methodology and applications. , 1985, Journal of cell science.

[26]  D. Axelrod.  Cell-substrate contacts illuminated by total internal reflection fluorescence , 1981, The Journal of cell biology.

[27]  Myung K. Kim,et al.  Wavelength scanning digital interference holography for variable tomographic scanning. , 2005, Optics express.

[28]  Daniel Axelrod,et al.  Total internal reflection fluorescent microscopy , 1983 .

[29]  A. S. G. Curtis,et al.  THE MECHANISM OF ADHESION OF CELLS TO GLASS , 1964, The Journal of cell biology.

[30]  Chung Ki Hong,et al.  Pixel-size-maintained image reconstruction of digital holograms on arbitrarily tilted planes by the angular spectrum method. , 2008, Applied optics.

[31]  Chun-Min Lo,et al.  High-resolution quantitative phase-contrast microscopy by digital holography. , 2005, Optics express.

[32]  D. Dormann,et al.  Imaging of cell migration , 2006, The EMBO journal.

[33]  B. Gumbiner,et al.  Regulation of cadherin-mediated adhesion in morphogenesis , 2005, Nature Reviews Molecular Cell Biology.