Transcriptome/Proteome Analysis of Corynebacterium glutamicum

Our understanding of Corynebacterium glutamicum has been revolutionized since its genome sequence, whole-genome DNA microarrays for transcriptome analysis, and proteomics techniques have become available. This chapter describes how DNA microarray-based transcriptome analyses as well as cytosolic and membrane proteomics have expedited the knowledge about the physiology and metabolic regulation of C. glutamicum with respect to fundamental and applied research. Recent examples of employing transcriptome analysis to study stimulons, transcriptional regulators, in particular of carbon metabolism and the stress response, to characterize in vivo-evolved strains, for pathway identification, genome instabilities, and strain development for amino acid production are given along with an outlook into future development of transcriptomics due to technical developments. The status quo of C. glutamicum proteomics by classical 2D gel electrophoresis and more recent techniques also allowing the study of membrane proteins are described. Emphasis is given to the posttranslational modification of C. glutamicum proteins by phosphorylation, glycosylation, and N-terminal processing. Selected examples of studying protein–DNA and protein–protein interactions are also discussed.

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