Ewald sphere correction for single-particle electron microscopy.

Most algorithms for three-dimensional (3D) reconstruction from electron micrographs assume that images correspond to projections of the 3D structure. This approximation limits the attainable resolution of the reconstruction when the dimensions of the structure exceed the depth of field of the microscope. We have developed two methods to calculate a reconstruction that corrects for the depth of field. Either method applied to synthetic data representing a large virus yields a higher resolution reconstruction than a method lacking this correction.

[1]  Ericka Stricklin-Parker,et al.  Ann , 2005 .

[2]  N Grigorieff,et al.  Three-dimensional structure of bovine NADH:ubiquinone oxidoreductase (complex I) at 22 A in ice. , 1998, Journal of molecular biology.

[3]  John E. Johnson,et al.  Virus Particle Explorer (VIPER), a Website for Virus Capsid Structures and Their Computational Analyses , 2001, Journal of Virology.

[4]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[5]  W. O. Saxton.  What is the focus variation method? Is it new? Is it direct? , 1994 .

[6]  R. H. Wade.  A brief look at imaging and contrast transfer , 1992 .

[7]  Xiaodong Yan,et al.  Structure and assembly of large lipid-containing dsDNA viruses , 2000, Nature Structural Biology.

[8]  M F Schmid,et al.  Estimates of validity of projection approximation for three-dimensional reconstructions at high resolution. , 1984, Ultramicroscopy.

[9]  A. Klug,et al.  Measurement and compensation of defocusing and aberrations by Fourier processing of electron micrographs , 1971 .

[10]  S. Harrison,et al.  Molecular model for a complete clathrin lattice from electron cryomicroscopy , 2004, Nature.

[11]  Y. Modis,et al.  Atomic model of the papillomavirus capsid , 2002, The EMBO journal.

[12]  A. Authier,et al.  Diffraction Physics , 1998 .

[13]  M Marko,et al.  Three-dimensional transmission electron microscopy and its application to mitosis research. , 1999, Methods in cell biology.

[14]  Marin van Heel,et al.  Similarity measures between images , 1987 .

[15]  W. Baumeister,et al.  Electron tomography of ice-embedded prokaryotic cells. , 1998, Biophysical journal.

[16]  D. DeRosier.  Correction of high-resolution data for curvature of the Ewald sphere. , 2000, Ultramicroscopy.

[17]  W Chiu,et al.  EMAN: semiautomated software for high-resolution single-particle reconstructions. , 1999, Journal of structural biology.

[18]  P. P. Ewald.  Die Berechnung optischer und elektrostatischer Gitterpotentiale , 1921 .

[19]  M van Heel,et al.  A new generation of the IMAGIC image processing system. , 1996, Journal of structural biology.