Temperature dependence of near-infrared spectra of whole blood.

The temperature dependence (30 to 40 degrees C) of near-infrared spectra (500 to 1100 nm) of whole human blood, including red blood cells with intact physiological function, is investigated. Previous studies have focused on hemoglobin solutions, but the operation of red blood cells is critically dependent on intact cell membranes to perform normal oxygen transport and other physiological functions. Thus measurements of whole blood are more directly related to changes that occur in vivo. In addition to the response of hemoglobin to temperature in the spectra, a temperature response from water in the plasma is also detected. The temperature response of the water absorption at 960 nm is approximately ten times smaller than the temperature response of the oxyhemoglobin component in the blood at 610 nm. However, it is the most significant temperature effect between 800 and 1000 nm. This work will aid the precision and understanding of full spectrum near-infrared measurements on blood.

[1]  Daniel X Hammer,et al.  Toward noninvasive measurement of blood hematocrit using spectral domain low coherence interferometry and retinal tracking. , 2006, Optics express.

[2]  H. Refsum,et al.  Spectrophotometric determination of hemoglobin oxygen saturation in hemolyzed whole blood. , 1956, Scandinavian journal of clinical and laboratory investigation.

[3]  M. Ferrari,et al.  Near infrared absorption spectra of human deoxy- and oxyhaemoglobin in the temperature range 20–40°C , 1997 .

[4]  A. Roggan,et al.  Optical Properties of Circulating Human Blood in the Wavelength Range 400-2500 nm. , 1999, Journal of biomedical optics.

[5]  Roland N. Pittman,et al.  In vivo photometric analysis of hemoglobin , 2006, Annals of Biomedical Engineering.

[6]  Q. Gibson,et al.  The origin of the Adams-Schuster difference spectrum of oxyhemoglobin. , 1975, Biochemical and biophysical research communications.

[7]  Erwin F Hirsch,et al.  Near-infrared spectrometric determination of blood pH. , 2002, The Journal of surgical research.

[8]  G. Millikan.  The Oximeter, an Instrument for Measuring Continuously the Oxygen Saturation of Arterial Blood in Man , 1942 .

[9]  M. Leone,et al.  Optical absorption spectra of deoxy- and oxyhemoglobin in the temperature range 300-20 K. Relation with protein dynamics. , 1986, Biophysical chemistry.

[10]  C. Cooper,et al.  Absolute quantification of deoxyhaemoglobin concentration in tissue near infrared spectroscopy. , 1994, Physics in medicine and biology.

[11]  A. M. Weindling,et al.  Measurement of venous oxyhaemoglobin saturation in the adult human forearm by near infrared spectroscopy with venous occlusion , 1997, Medical and Biological Engineering and Computing.

[12]  T. Kenner,et al.  The measurement of blood density and its meaning , 1989, Basic Research in Cardiology.

[13]  J. Rifkind,et al.  Temperature dependent spectral changes of iron and nickel hemoglobins and their derivatives. , 1985, Biochemical and biophysical research communications.

[14]  M. Friebel,et al.  Determination of the complex refractive index of highly concentrated hemoglobin solutions using transmittance and reflectance measurements. , 2005, Journal of biomedical optics.

[15]  T. M. Schuster,et al.  Phosphate-dependent spectroscopic changes in liganded hemoglobin. , 1974, Biochemical and biophysical research communications.

[16]  J M Steinke,et al.  Effects of temperature on optical absorbance spectra of oxy-, carboxy-, and deoxyhemoglobin. , 1992, Clinical chemistry.

[17]  B. Horecker.  THE ABSORPTION SPECTRA OF HEMOGLOBIN AND ITS DERIVATIVES IN THE VISIBLE AND NEAR INFRA-RED REGIONS , 1943 .

[18]  R. C. Weast.  CRC Handbook of Chemistry and Physics , 1973 .

[19]  P. L. San Biagio,et al.  Temperature induced difference spectra of oxy and deoxy hemoglobin in the near IR, visible and Soret regions. , 1977, Biochemical and biophysical research communications.