Femtosecond pulse-induced microprocessing of live Drosophila embryos

We characterized the effect of femtosecond pulse trains on gastrulating Drosophila embryos using two-photonexcited fluorescence (2PEF) and third-harmonic generation (THG) microscopy. Femtosecond pulses can be used to perform controlled intravital microdissections that alter the embryo structural integrity but do not significantly perturb cytoskeleton dynamics in adjacent cells. Such targeted ablations can be used to remotely perturb cell movements in developing embryos. The transparent combination of femtosecond pulse-induced ablation with nonlinear microscopy can be used to analyze in vivo the effect of cell and tissue deformations. r 2005 Elsevier GmbH. All rights reserved.

[1]  John White,et al.  Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability , 1999, Nature Biotechnology.

[2]  Bruno Moulia,et al.  Velocimetric third-harmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryos. , 2004, Optics letters.

[3]  R. Karess,et al.  Reassessing the role and dynamics of nonmuscle myosin II during furrow formation in early Drosophila embryos. , 2003, Molecular biology of the cell.

[4]  P. O’Farrell,et al.  A nuclear GFP that marks nuclei in living Drosophila embryos; maternal supply overcomes a delay in the appearance of zygotic fluorescence. , 1995, Developmental biology.

[5]  K R Wilson,et al.  Third harmonic generation microscopy. , 1998, Optics express.

[6]  Kazuyoshi Itoh,et al.  Femtosecond laser disruption of subcellular organelles in a living cell. , 2004, Optics express.

[7]  M Deutsch,et al.  Second-harmonic microscopy of biological tissue. , 1986, Optics letters.

[8]  Ward Small,et al.  Plasma mediated ablation of biological tissues with nanosecond-to-femtosecond laser pulses: relative role of linear and nonlinear absorption , 1996 .

[9]  E. Wieschaus,et al.  src64 and tec29 are required for microfilament contraction during Drosophila cellularization , 2004, Development.

[10]  X. Xie,et al.  Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications , 2004 .

[11]  M. Leptin,et al.  Gastrulation in Drosophila: the logic and the cellular mechanisms , 1999, The EMBO journal.

[12]  Karsten König,et al.  Cell biology: Targeted transfection by femtosecond laser , 2002, Nature.

[13]  U. Parlitz,et al.  Energy balance of optical breakdown in water at nanosecond to femtosecond time scales , 1999 .

[14]  Rustem F. Ismagilov,et al.  Dynamics of Drosophila embryonic patterning network perturbed in space and time using microfluidics , 2005, Nature.

[15]  Emmanuel Farge,et al.  Is mechano‐sensitive expression of twist involved In mesoderm formation? , 2004, Biology of the cell.

[16]  K König,et al.  Nanodissection of human chromosomes with near-infrared femtosecond laser pulses. , 2001, Optics letters.

[17]  Mehmet Fatih Yanik,et al.  Neurosurgery: Functional regeneration after laser axotomy , 2004, Nature.

[18]  D. Kleinfeld,et al.  All-Optical Histology Using Ultrashort Laser Pulses , 2003, Neuron.

[19]  D W Tank,et al.  Direct Measurement of Coupling Between Dendritic Spines and Shafts , 1996, Science.

[20]  R. Reuter,et al.  A role for the mesoderm in endodermal migration and morphogenesis in Drosophila. , 1993, Development.

[21]  Iris Riemann,et al.  Intratissue surgery with 80 MHz nanojoule femtosecond laser pulses in the near infrared. , 2002, Optics express.

[22]  Wayne L. Rickoll,et al.  Multiple Forces Contribute to Cell Sheet Morphogenesis for Dorsal Closure in Drosophila , 2000, The Journal of cell biology.

[23]  Alan Sugar,et al.  Ultrafast (femtosecond) laser refractive surgery. , 2002, Current opinion in ophthalmology.

[24]  Bruno Moulia,et al.  In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[25]  M D Duncan,et al.  Scanning coherent anti-Stokes Raman microscope. , 1982, Optics letters.

[26]  Tsung-Han Tsai,et al.  Higher harmonic generation microscopy for developmental biology. , 2004, Journal of structural biology.

[27]  E. Mazur,et al.  Bulk heating of transparent materials using a high-repetition-rate femtosecond laser , 2003 .

[28]  E. Neher,et al.  Highly nonlinear photodamage in two-photon fluorescence microscopy. , 2001, Biophysical journal.

[29]  E. Farge.  Mechanical Induction of Twist in the Drosophila Foregut/Stomodeal Primordium , 2003, Current Biology.

[30]  G. Edwards,et al.  Forces for Morphogenesis Investigated with Laser Microsurgery and Quantitative Modeling , 2003, Science.

[31]  Jeffrey A. Squier,et al.  Third-harmonic generation imaging of laser-induced breakdown in glass , 1999 .

[32]  Shyamsunder Erramilli,et al.  Confocal and Two-Photon Microscopy—Foundations, Applications and Advances , 2004 .

[33]  Yaron Silberberg,et al.  Nonlinear scanning laser microscopy by third harmonic generation , 1997 .

[34]  E. Brouzes,et al.  Interplay of mechanical deformation and patterned gene expression in developing embryos. , 2004, Current opinion in genetics & development.

[35]  W. Webb,et al.  Nonlinear magic: multiphoton microscopy in the biosciences , 2003, Nature Biotechnology.

[36]  Jerome Mertz,et al.  Nonlinear microscopy: new techniques and applications , 2004, Current Opinion in Neurobiology.

[37]  Yaron Silberberg,et al.  Depth-resolved structural imaging by third-harmonic generation microscopy. , 2004, Journal of structural biology.

[38]  Karsten König,et al.  Femtosecond near-infrared laser pulses as a versatile non-invasive tool for intra-tissue nanoprocessing in plants without compromising viability. , 2002, The Plant journal : for cell and molecular biology.

[39]  Renzo Antolini,et al.  Combined intracellular three-dimensional imaging and selective nanosurgery by a nonlinear microscope. , 2005, Journal of biomedical optics.

[40]  D E Ingber,et al.  Pulse energy dependence of subcellular dissection by femtosecond laser pulses. , 2005, Optics express.

[41]  J Mertz,et al.  Odor-evoked calcium signals in dendrites of rat mitral cells. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[42]  James A Galbraith,et al.  Controlled damage in thick specimens by multiphoton excitation. , 2003, Molecular biology of the cell.

[43]  Michael D. Cahalan,et al.  Two-photon tissue imaging: seeing the immune system in a fresh light , 2002, Nature Reviews Immunology.

[44]  S W Hell,et al.  Heating by absorption in the focus of an objective lens. , 1998, Optics letters.

[45]  K J Halbhuber,et al.  Ultrastructure and reproduction behaviour of single CHO-K1 cells exposed to near infrared femtosecond laser pulses. , 2006, Scanning.

[46]  K. Fujita.  [Two-photon laser scanning fluorescence microscopy]. , 2007, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.

[47]  Vladimir P Torchilin,et al.  Quantum dots spectrally distinguish multiple species within the tumor milieu in vivo , 2005, Nature Medicine.