Studies on the Interaction between Three Small Flavonoid Molecules and Bovine Lactoferrin

The interaction between three flavonoids, i.e., Luteolin (LTL), Quercetin (QCT), and Naringenin (NGN) and bovine lactoferrin (BLF) at pH 7.4 was investigated by fluorescence quenching spectra, synchronous fluorescence spectra, and UV-visible absorption spectra. The results indicate the fluorescence of BLF quenched by Luteolin (LTL), Quercetin (QCT), and Naringenin (NGN) via static quenching. The main force between QCT and LTL with BLF was van der Waals interactions and hydrogen bonds. Electrostatic interactions played a major role in the binding process of interaction between NGN and BLF. Synchronous fluorescence was used to study the conformational changes of BLF. The values of binding constant (Ka) and number of binding sites (n) at different temperatures (300K, 305K, 310K) were also calculated, respectively. The results of corresponding thermodynamic parameters as well as binding distance between BLF and LTL, QCT, or GNG were obtained. These results implied that Luteolin (LTL), Quercetin (QCT), and Naringenin (NGN) could provide important guides for compound quantity (e.g., medicine dosage) and the design of new compounds (or drugs).

[1]  M. Faldyna,et al.  Lactoferrin: a review , 2018 .

[2]  Xin Wang,et al.  Studies on the competitive binding of cleviprex and flavonoids to plasma protein by multi-spectroscopic methods: A prediction of food-drug interaction. , 2017, Journal of photochemistry and photobiology. B, Biology.

[3]  J. Chamani,et al.  Study on effect of lomefloxacin on human holo-transferrin in the presence of essential and nonessential amino acids: Spectroscopic and molecular modeling approaches. , 2017, International journal of biological macromolecules.

[4]  P. Brodelius,et al.  Comparison of the interaction between lactoferrin and isomeric drugs. , 2017, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[5]  J. Chamani,et al.  Study of the interaction between DNP and DIDS with human hemoglobin as binary and ternary systems: spectroscopic and molecular modeling investigation , 2016, Journal of biomolecular structure & dynamics.

[6]  Chenqi Xu,et al.  Molecular interaction between (-)-epigallocatechin-3-gallate and bovine lactoferrin using multi-spectroscopic method and isothermal titration calorimetry. , 2014, Food research international.

[7]  Huiyuan Guo,et al.  Bovine lactoferrin binds oleic acid to form an anti-tumor complex similar to HAMLET. , 2014, Biochimica et biophysica acta.

[8]  T. Efferth,et al.  Differential interactions of the broad spectrum drugs artemisinin, dihydroartemisinin and artesunate with serum albumin. , 2013, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[9]  J. Chamani,et al.  Studies on the Antagonistic Behavior Between Cyclophosphamide Hydrochloride and Aspirin with Human Serum Albumin: Time-Resolved Fluorescence Spectroscopy and Isothermal Titration Calorimetry , 2013, Journal of Solution Chemistry.

[10]  X. Dai,et al.  Resveratrol Binding to Fibrinogen and its Biological Implication , 2012, Food Biophysics.

[11]  A. Lamb,et al.  Recent advances in understanding the antibacterial properties of flavonoids. , 2011, International journal of antimicrobial agents.

[12]  Lili Yan,et al.  Spectroscopic and voltammetric characterizations of the interaction of two local anesthetics with bovine serum albumin , 2011 .

[13]  M. Guo,et al.  Analysis of the spectroscopic characteristics on the binding interaction between tosufloxacin and bovine lactoferrin , 2011 .

[14]  J. Chamani,et al.  Comparing the interaction of cyclophosphamide monohydrate to human serum albumin as opposed to holo-transferrin by spectroscopic and molecular modeling methods: evidence for allocating the binding site. , 2010, Protein and peptide letters.

[15]  J. Chamani,et al.  Characterization of the interaction between human lactoferrin and lomefloxacin at physiological condition: Multi-spectroscopic and modeling description , 2010 .

[16]  Julie Schappler,et al.  Drug–protein binding: a critical review of analytical tools , 2010, Analytical and bioanalytical chemistry.

[17]  M. Hillebrand,et al.  Interaction of kaempferol with human serum albumin: a fluorescence and circular dichroism study. , 2010, Journal of pharmaceutical and biomedical analysis.

[18]  C. Zhao,et al.  Study on the interaction between 3 flavonoid compounds and alpha-amylase by fluorescence spectroscopy and enzymatic kinetics. , 2009, Journal of food science.

[19]  S. Nagini,et al.  In vitro evaluation of the anticancer effect of lactoferrin and tea polyphenol combination on oral carcinoma cells , 2007, Cell biology international.

[20]  Andrew J Lamb,et al.  Antimicrobial activity of flavonoids , 2005, International Journal of Antimicrobial Agents.

[21]  M. Xie,et al.  Interaction between hesperetin and human serum albumin revealed by spectroscopic methods. , 2005, Biochimica et biophysica acta.

[22]  Liu Yuan,et al.  Spectroscopic studies on the interaction of cinnamic acid and its hydroxyl derivatives with human serum albumin , 2004 .

[23]  R. Eckenhoff,et al.  Molecular interactions between inhaled anesthetics and proteins. , 1997, Pharmacological reviews.

[24]  W. Hager,et al.  and s , 2019, Shallow Water Hydraulics.

[25]  Theresa Ibibia.  A Review on Antimicrobial and Other Beneficial Effects of Flavonoids , 2013 .

[26]  W. Marsden.  I and J , 2012 .

[27]  H. Tajmir-Riahi,et al.  Interaction of β-Lactoglobulin with Resveratrol and its Biological Implications , 2008 .

[28]  C. Sorenson,et al.  Electrochemical Evidence for the Molten Globule States of Cytochrome c Induced by N-Alkyl Sulfates at Low Concentrations , 2003, Journal of protein chemistry.

[29]  Maurice R. Eftink,et al.  Fluorescence Quenching: Theory and Applications , 2002 .