Sub-millisecond time-resolved SAXS using a continuous-flow mixer and X-ray microbeam

The development of a high-duty-cycle microsecond time-resolution SAXS capability at the Biophysics Collaborative Access Team beamline (BioCAT) 18ID at the Advanced Photon Source, Argonne National Laboratory, USA, is reported.

[1]  K. Hodgson,et al.  The Radius of Gyration of an Apomyoglobin Folding Intermediate , 1995, Science.

[2]  T. Weiss,et al.  Dynamics of the self-assembly of unilamellar vesicles. , 2005, Physical review letters.

[3]  H. Roder,et al.  Rapid mixing methods for exploring the kinetics of protein folding. , 2004, Methods.

[4]  S. Woodson,et al.  Compact intermediates in RNA folding. , 2010, Annual review of biophysics.

[5]  Everett A Lipman,et al.  Single-Molecule Measurement of Protein Folding Kinetics , 2003, Science.

[6]  Mixing liquids in microseconds , 1985 .

[7]  B. Krauskopf,et al.  Proc of SPIE , 2003 .

[8]  D. Svergun,et al.  Small-angle scattering studies of biological macromolecules in solution , 2003 .

[9]  Satoshi Takahashi,et al.  Conformational landscape of cytochrome c folding studied by microsecond-resolved small-angle x-ray scattering , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[10]  T. Irving,et al.  The BioCAT undulator beamline 18ID: a facility for biological non-crystalline diffraction and X-ray absorption spectroscopy at the Advanced Photon Source. , 2004, Journal of synchrotron radiation.

[11]  Tobin R Sosnick,et al.  The folding of single domain proteins--have we reached a consensus? , 2011, Current opinion in structural biology.

[12]  T. Paunesku,et al.  Fast-scanning high-flux microprobe for biological X-ray fluorescence microscopy and microXAS. , 2010, Journal of synchrotron radiation.

[13]  P. Zamore,et al.  Argonaute divides its RNA guide into domains with distinct functions and RNA-binding properties. , 2012, Cell.

[14]  Robert K Prud'homme,et al.  Mechanism for rapid self-assembly of block copolymer nanoparticles. , 2003, Physical review letters.

[15]  C. Teschke,et al.  SecA folding kinetics: a large dimeric protein rapidly forms multiple native states. , 2004, Journal of molecular biology.

[16]  H. Roder,et al.  Methods for exploring early events in protein folding. , 1999, Current opinion in structural biology.

[17]  E. Kondrashkina,et al.  Microsecond acquisition of heterogeneous structure in the folding of a TIM barrel protein , 2008, Proceedings of the National Academy of Sciences.

[18]  Osman Bilsel,et al.  Minireview: structural insights into early folding events using continuous-flow time-resolved small-angle X-ray scattering. , 2011, Biopolymers.

[19]  Peter J. Eng,et al.  Dynamically figured Kirkpatrick Baez x-ray microfocusing optics , 1998, Optics & Photonics.

[20]  C. Matthews,et al.  A microchannel solution mixer for studying microsecond protein folding reactions , 2005 .

[21]  P. Goodwin,et al.  A two-dimensional view of the folding energy landscape of cytochrome c. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[22]  D. Thirumalai,et al.  Early events in RNA folding. , 2001, Annual review of physical chemistry.

[23]  E. Henry,et al.  [8] Singular value decomposition: Application to analysis of experimental data , 1992 .

[24]  V. Guida Thermodynamics and kinetics of vesicles formation processes. , 2010, Advances in colloid and interface science.

[25]  W. Royer,et al.  Time-resolved x-ray crystallography of heme proteins. , 2008, Methods in enzymology.

[26]  W. Eaton,et al.  Protein folding studied by single-molecule FRET. , 2008, Current opinion in structural biology.

[27]  T. Irving,et al.  Multistage collapse of a bacterial ribozyme observed by time-resolved small-angle X-ray scattering. , 2010, Journal of the American Chemical Society.

[28]  E. Elson,et al.  Measurement of microsecond dynamic motion in the intestinal fatty acid binding protein by using fluorescence correlation spectroscopy , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[29]  E. Kondrashkina,et al.  Microsecond Hydrophobic Collapse in the Folding of Escherichia coli Dihydrofolate Reductase, an α/β-Type Protein , 2007 .

[30]  H. Roder,et al.  Evidence for barrier-limited protein folding kinetics on the microsecond time scale , 1998, Nature Structural Biology.

[31]  J. Hofrichter,et al.  Submillisecond protein folding kinetics studied by ultrarapid mixing. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[32]  G. G. Stokes "J." , 1890, The New Yale Book of Quotations.