Widespread Distribution of the 14‐3‐3 Protein in Vertebrate Brains and Bovine Tissues: Correlation with the Distributions of Calcium‐Dependent Protein Kinases

Abstract: A highly specific antiserum was prepared against bovine brain 14‐3‐3 protein, a protein kinase‐dependent activator of tyrosine and tryptophan hydroxylases. The immunoassay using this antiserum proved the presence of 14‐3‐3 protein in various bovine tissues and in brains of various vertebrate species. The quantitative analysis indicated that the tissue distribution of 14‐3‐3 protein is more closely related to the known distributions of the Ca2+‐dependent protein kinases, i.e., Ca2+/calmodulin‐ and Ca2+/phospholipid‐dependent protein kinases, rather than those of tyrosine and tryptophan hydroxylases. This result, together with the available data on this protein, suggests potential roles of the 14‐3‐3 protein in more diverse kinasemediated processes than the predicted role in monoamine synthesis.

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