Stimulation of in vitro translation of messenger RNA by actinomycin D and cordycepin.

Actinomycin D and cordycepin were tested for their effect on translation in the wheat germ embryo extract and reticulocyte lysate assays for in vitro protein synthesis. Both drugs were found to stimulate the incorporation of 35S-labeled methionine into protein up to threefold as compared to control assays. This was true for synthesis directed by murine myeloma polyadenylate-containing RNA, tobacco mosaic virus RNA, and endogenous reticulocyte messenger RNA.

[1]  R. Raff,et al.  Protein synthesis and messenger RNA stability in activated, enucleate sea urchin eggs are not affected by actinomycin D. , 1976, Developmental biology.

[2]  G. Tomkins,et al.  “Superinduction” of tyrosine aminotransferase by actinomycin D: a reevaluation , 1975, Cell.

[3]  S. Penman,et al.  Regulation of protein synthesis in mammalian cells. V. Further studies on the effect of actinomycin D on translation control in HeLa cells. , 1973, Journal of molecular biology.

[4]  B. Paterson,et al.  Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[5]  R. Palmiter,et al.  Regulation of protein synthesis in chick oviduct. 3. Mechanism of ovalbumin "superinduction" by actinomycin D. , 1973, The Journal of biological chemistry.

[6]  P. Leder,et al.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[7]  J. Darnell,et al.  Polyadenylic Acid Sequences: Role in Conversion of Nuclear RNA into Messenger RNA , 1971, Science.

[8]  D. Granner,et al.  Superinduction of tyrosine aminotransferase by actinomycin D in rat hepatoma (HTC) cells. , 1970, Journal of molecular biology.

[9]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[10]  H. Lodish,et al.  Regulation of in vitro translation of bacteriophage f2 RNA. , 1969, Cold Spring Harbor symposia on quantitative biology.

[11]  H. Hiatt,et al.  THE STABILITY OF LIVER MESSENGER RNA. , 1964, Proceedings of the National Academy of Sciences of the United States of America.